Summary
The localization of oxidoreductases and transport enzymes in flask cells of the amphibian epidermis was studied at the light-microscopic level. In these cells, the deposition of cytochemical reaction products was very similar to that found in fish epidermal ionocytes, thus demonstrating histochemical similarities between these two types of cells. The present histochemical results revealed high levels of activity of alkaline phosphatase (ALPase), potassium-dependent nitrophenylphosphatase (K+-p-NPPase) and carbonic-anhydrase isozymes (CA-I and CA-II) in the apical region of the flask cells, indicating that enzyme zonation may be the main site of the ion pumping.
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References
Barka MD, Anderson PJ (1963) Histochemistry, theory, practice and bibliography. Harper and Row, New York
Bornancin M, de Renzis G, Naon R (1980) Cl−-HCO 3− -ATPase in gills of the rainbow trout: evidence for its microsomal localization. Am J Physiol 7:R251-R259
Brown D, Grosso A, De Sousa RC (1981) The amphibian epidermis: distribution of mitochondria-rich cells and the effect of oxytocin. J Cell Sci 52:197–213
Budtz PE, Larsen PO (1973) Structure of the toad epidermis during the moulting cycle. I. Light microscopic observations in Bufo bufo (L). Z Zellforsch 144:353–368
Budtz PE, Larsen PO (1975) Structure of the toad epidermis during the moulting cycle. II. Electron microscopic observations on Bufo bufo (L). Cell Tissue Res 159:459–483
Crabbé J (1964) Stimulation by aldosterone of active sodium transport across the isolated ventral skin of Amphibia. Endocrinology 75:809–811
De Bortoli M, Giunta C, Stacchini A, Sanchini M (1982) Na, K ATPase from Xenopus laevis kidney and epidermis: ouabain interaction studies. Boll Soc Jt Biol Sper 23:1525–1540
Ernst SA, Hootman SR (1981) Microscopical methods for the localization of Na+-K+-ATPase. Histochem J 13:397–418
Fox H (1983a) The skin of Ichthyophis (Amphibia: Caecilia): an ultrastructural study. J Zool Lond 199:223–248
Fox H (1983b) Amphibian morphogenesis. Humana Press, Clifton, New Jersey, pp 147–175
Greven H (1980) Ultrastructural investigations of the epidermis and the gill epithelium in the intrauterine larvae of Salamandra salamandra (L). Z mikrosk anat Forsch 94:196–208
Guardabassi A, Campantico E, Olivero M (1972) Effect of environmental changes on the skin and pituitary of Xenopus laevis Daudin specimens treated and untreated with prolactin. Monit Zool Ital 6:129–146
Hootman SR, Philpott CW (1979) Ultracytochemical localization of Na+, K+-activated ATPase in chloride cells from the gills of a euryhaline teleost. Anat Rec 193:99–129
Katz U, Larsen EH (1984) Chloride transport in toad skin (Bufo viridis). The effect of salt adaptation. J Exp Biol 109:353–371
Kugler P, Wrobel KH (1978) Studies on the optimalisation and standardisation of the light microscopical succinate dehydrogenase histochemistry. Histochemistry 57:47–60
Kumpulainen T (1984) Immunohistochemical localization of human carbonic anhydrase isozymes. Ann NY Acad Sci 429:359–368
Lavker RM (1972) Fine structure of the newt epidermis. Tissue Cell 3:663–675
Lewinson D, Rosenberg M, Warburg MR (1982) Mitochondriatich cells in salamander larva epidermis: ultrastructural description and carbonic anhydrase activity. Biol Cell 46:75–84
Lewinson D, Rosenberg M, Warburg MR (1984) “Chloride-cell”-like mitochondria-rich cells of salamander larva gill epithelium. Experientia 40:956–958
Lodi G (1971) Histoenzymologic characterization of the flask cells in the skin of the crested newt under normal and experimental conditions. Atti Acc Sci (Torino) 105:561–570
Maetz J, Jard S, Morel F (1958) Action de l'aldosterone sur le transport actif de sodium de la peau de grenouille. CR Acad Sci 247:516–518
Marshall WS, Nishioka RS (1980) Relation of mitochondria-rich cells to active chloride transport in the skin of a marine teleost. J Exp Zool 214:147–156
Masoni A, Garcia-Romeu F (1979) Moulting in Rana esculenta: development of mitochondria-rich cells, morphological changes of the epithelium and sodium transport. Cell Tissue Res 197:23–38
McGadey J (1970) A tetrazolium method for nonspecific alkaline phosphatase. Histochemie 23:180–184
Mayahara H, Fujimoto K, Ando T, Ogawa K (1980) A new onestep method for the cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenyl-phosphatase activity. Histochemistry 67:125–138
Meijer AEFH, de Vries GP (1974) Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. IV. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase (decarboxylating). Histochemistry 40:349–359
Meijer AEFH, de Vries GP (1975) Semipermeable membranes for improving the histochemical demonstration of enzyme activities in tissue sections. V. Isocitrate: NADP+ oxidoreductase (decarboxylating) and malate: NADP+ oxidoreductase (decarboxylating). Histochemistry 43:225–236
Mills JW (1981) Autoradiography of diffusible substances: verfication of the specificity of the localization by correlated physiological, biochemical, and pharmacological studies. J Histochem Cytochem 29:136–142
Nachlas M, Tsou KC, De Souza E, Cheng CS, Seligman AM (1957) The cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J Histochem Cytochem 5:420–436
Rosen S, Friedley NJ (1973) Carbonic anhydrase activity in Rana pipiens skin: biochemical and histochemical analysis. Histochemie 36:1–4
Sternberger LA (1979) Immunohistochemistry. 2nd edn. John Wiley & Sons, New York, pp 104–130
Voûte CL, Thummel J, Brenner M (1975) Aldosterone effect in the epithelium of the frog — A new story about an old enzyme. J Steroid Biochem 6:1175–1179
Wattenberg LW, Leong JL (1960) Effects of coenzyme Q10 and menadione on succinic dehydrogenase activity as measured by tetrazolium salt reduction. J Histochem Cytochem 8:296–303
Whitear M (1975) Flask cells in epidermal dynamics in frog skin. J Zool (Lond) 175:107–149
Whitear (1977) A functional comparison between the epidermis of fish and amphibians. Symp Zool Soc Lond 39:291–313
Zaccone G (1983) Histochemical studies of acid proteoglycans and glycoproteins and activities of hydrolytic and oxidoreductive enxymes in the skin epidermis of the fish Blennius sanguinolentus Pallas (Teleostei: Bleniidae). Histochemistry 78:163–175
Zaccone G, Fasulo S, Licata A (1984) Ultrastructural demonstration of alkaline phosphatase (ALP) and K+-p-nitrophenyl phosphatase (K+-p-Nppase) in the epidermal ionocytes of Blennius sanguinolentus. Histochemistry 81:47–53
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Zaccone, G., Fasulo, S., Lo Cascio, P. et al. Enzyme cytochemical and immunocytochemical studies of flask cells in the amphibian epidermis. Histochemistry 84, 5–9 (1986). https://doi.org/10.1007/BF00493412
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DOI: https://doi.org/10.1007/BF00493412