Abstract
Yellow-pigmented bacteria showing typical characteristics of Xanthobacter spp. were isolated from enrichments with propene and 1-butene, using classical techniques. The generation time for growth on propene and 1-butene of these bacteria ranged from 5 to 7h. A NADH-dependent mono-oxygenase was identified in cell-free extract of Xanthobacter Py2. This mono-oxygenase was not influenced by potential inhibitors tested indicating that propene mono-oxygenase is different from other hydrocarbon mono-oxygenases described until now. Nitrogenase activity could be measured using the acetylene reduction assay with propene as energy source, because acetylene did not inhibit the mono-oxygenase activity.
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van Ginkel, C.G., de Bont, J.A.M. Isolation and characterization of alkene-utilizing Xanthobacter spp.. Arch. Microbiol. 145, 403–407 (1986). https://doi.org/10.1007/BF00470879
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DOI: https://doi.org/10.1007/BF00470879