Abstract
2-, 3-, and 4-chloroaniline degrading bacteria were obtained by natural genetic exchange between an aniline or toluidine degrading Pseudomonas strain and the chlorocatechol assimilating Pseudomonas sp. B13. Hybrid organisms were isolated through cocultivation of the parent strains in the chemostat as well as through conjugation on solid media in presence of chloroanilines as the selective substrates. Biochemical analysis of the gene products in the hybrid strains clearly showed that the genes coding for the aniline dioxygenase or the genes for the chlorocatechol assimilatory sequence had been transferred.
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Latorre, J., Reineke, W. & Knackmuss, HJ. Microbial metabolism of chloroanilines: enhanced evolution by natural genetic exchange. Arch. Microbiol. 140, 159–165 (1984). https://doi.org/10.1007/BF00454919
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DOI: https://doi.org/10.1007/BF00454919