Summary
By using batch-culture enrichment techniques a mixed culture of two bacterial spe cies identified as Cellulomonas flavigena and Xanthomonas sp was isolated. The capacity of both bacteria to grow as pure cultures in a min eral medium with alkaline pretreated sugar cane bagasse or cellobiose was tested. C. flavigena as pure culture was able to grow on both substrates only when yeast extract or biotin and thiamine were added to the culture medium, while Xanthomonas sp. could not grow on sugar cane ba gasse, but assimilated cellobiose if yeast extract was supplied. However, both bacteria in mixed culture grew very well on both substrates and did not require any growth factor. It was concluded that the interaction was favourable to both species. The mixed culture had the capacity to degrade a number of different agricul tural wastes and to use them as the sole carbon and energy source for the production mainly of biomass. More than 80% of pineapple bagasse, without chemical pretreatment, was used up by the microbial system.
Article PDF
Avoid common mistakes on your manuscript.
References
Callihan CD, Cunlap CE (1971) Construction of a chemical-microbial pilot plant for production of single cell protein from cellulosic wastes. Report PB 203620 National Technical Information Service, US Department of Commerce
De la Torre M (1981) Producción de proteínas alimenticias de origen unicelular en residuos celulósicos. Ph D Thesis, ENCB Instituto Poli técnico Nacional, México
Dye DW (1962) The inadequacy of the usual deter minative test for the identification of Xanthomonas spp. New Zealand J Science 5 (4): 393–416
Enriquez A, Montalvo R, Canales C (1981) Variation of bagasse crystallinity and cellulase activity during the fermentation of Cellulom o nas bacteria Biotechnol Bioeng 23 (7): 1431–1436
Fredrickson AB (1977) Behavior or mixed cultures of microorganism Ann Rev Microbiol 31: 63–87
Han YW (1982) Nutritional requirements and growth of a Cellulomonas species on cellulosic substrates J Ferment Technol 60 (2): 99–104
Han YW, Srinivasan VR (1968) Isolation and characterization of a cellulose utilizing bacterium Appl Microbiol 31: 63–68
Harrison DEF, Wreng SJ (1977) Mixed microbial cultures as a basis for future fermentation processes. Process Biochem 11(8): 30–33
Harrison DEF (1978) Mixed cultures in industrial fermentation processes Adv Appl Microbiol 24:129–164
Kristensen TD (1978) Continuous single cell protein production from Cellulomonas sp. and Candida utilis grown in mixture on barley straw European J Appl Microbiol Biotechnol 5: 155–163
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin-phenol reagent J Biol Chem 193: 265–275
Mateles LR (1979) Biotechnology in SCP production: is pure culture operation desirable? Procc GIAM-V: 35–319
Molina ED (1980) Producción de proteína unicelu lar usando como fuente de hidratos de carbono medula de bagazo Ph D Thesis, Universidad Nacional de Tucumán, Argentina
Moo-Young M, Campbell WR (Eds) (1981) Advances in Biotechnology, Vol II, Proceedings of the 6th International Fermentation Symposium. Pergamon Press. Canada
Tseng MMC, Phillips CR (1981) Mixed cultures comensalism and competition with Proteus vu l garis and Saccharomyces cerevisiae Biotechnol Bioeng 23: 1639–1651
Peitersen N (1975) Cellulase and protein produc tion from mixed cultures of Trichoderma viride and a yeast. Biotechnol Bioeng 17: 1291–1299
Perotti de Galvez IN, Molina EO (1981) Influence of the burning of sugar cane bagasse prior to its harvesting upon the production of single cell protein from bagasse pith Biotech not Lett 3 (12): 717–722
Poincelot DP, Day PR (1971) Simple dye release assay for determining cellulolytic activity for fungi. Appl Microbiol 23 (5): 875–879
Rolz C, Humphrey A (1982) Microbial biomass from renewables: review of alternatives Adv Biochem Eng 21: 1–54
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de la Torre, M., Campillo, C.C. Isolation and characterization of a symbiotic cellulolytic mixed bacterial culture. Appl Microbiol Biotechnol 19, 430–434 (1984). https://doi.org/10.1007/BF00454383
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00454383