Abstract
Twenty-five patients were examined in vivo with 99mTc labelled monoclonal antibodies; 15 with suspected infections with an antigranulocyte antibody (BW 250/183), 10 with suspected recurrence of a colorectal carcinoma with an anti CEA antibody (BW 431/26). Both antibodies were IgG1 isotypes. In the patients with suspected infections no change of the peripheral leukocyte count could be observed after the antibody injection (1 mg, n=9; 0.5 mg, n=1; 0.25 mg, n=6). In 2 patients examined with the anti CEA antibody (2 mg), a significant decrease of the peripheral leukocyte count could be observed. The recovery rate of the 99mTc antibody labelled granulocytes was calculated to be about 10%. The increase of the antibody-antigen binding was calculated to be 0.2%/min. In vivo the organ distribution curves demonstrated an increase of 99mTc activity over spleen and bone marrow of 1.1%/min, which was interpreted as antigen-antibody reactivity. The organ distribution curves of the anti granulocyte antibody over spleen and bone marrow showed typical binding characteristics to the local granulocyte epitopes. The curves over other organs showed a simple perfusion pattern. The curves of the anti CEA antibody showed a perfusion pattern over all the examined organs. A sham dialysis model in one patient with renal insufficiency undergoing regular dialysis treatment demonstrated the viability of 99mTc antibody labelled granulocytes in vivo. The kinetic patterns of the 99mTc antibody in patients with Crohn's disease were interpreted as CEA binding of the antibody in the bowel wall.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Becker W (1988) Leukozytenszingtigraphie zur Diagnostik entzündlicher Erkrankungen. GIT-Darmstadt
Becker W, Fischbach W, Reiners C, Börner W (1986) Three phase white blood cell scan: its diagnostic accuracy in abdominal inflammatory diseases. J Nucl Med 27:1109–1115
Becker W, Schäfer R, Börner W (1988a) In vivo viability of In-111 labelled granulocytes demonstrated in a sham-dialysis model. Br J Radiol (in press)
Becker W, Schomann E, Fischbach W, Börner W, Gruner KR (1988b) Comparison of Tc-99m-HMPAO and In-111-oxine labelled granulocytes in man: First clinical results. Nucl Med Commun 9:435–447
Bosslet K, Lüben G, Schwarz A, Hundt E, Harthus HP, Seiler FR, Muhrer C, Klöppel G, Kayser K, Sedlacek HH (1985) Immunohistochemical localisation and molecular characteristics of three monoclonal antibody — defined epitopes detectable on carcinoembryonic antigen (CEA). Int J Cancer 36:75–84
Coleman RE (1982) Radiolabelled leukocytes. Nuclear Medicine Annual, pp 119–141
Dillman RO, Beauregard JC, Sobol RE, Royston I, Bartholomew RM, Hagan PS, Halpern SE (1984) Lack of radioimmunodetection and complications associated with monoclonal anticarcinoembrionic antigen antibody cross reactivity with an antigen on circulating cells. Cancer Res 44:2213–2218
Fischbach W (1988) Gewebekonzentrationen der tumorassoziierten Antigene CEA und CA 19-9 und DNA-Aneuploidie in der kolorektalen Karzinogenese. Habilitationsschrift an der Julius-Maximilians-Universität Würzburg
Gross R (1981) Tumormarker in der inneren Medizin. In: Uhlenbruck U, Wintzer G (eds) CEA und andere Tumormarker. Tumordiagnostik, Leonberg, pp 11–21
Hauser MF, Alderson PO (1978) Gallium-67 imaging in abdominal disease. Sem Nucl Med 8:251–270
Iles SE, Ehrlioch LE, Saliken JK, Martin RH (1987) Indium-111 chloride scintigraphy in adult osteomyelitis. J Nucl Med 28:1540–1545
Joseph K, Höffken H, Damann V (1987) In-vivo Markierung von Granulozyten mit Tc-99m markierten monoklonalen Antikörpern: erste klinische Ergebnisse. Nuccompact 18:223–226
Locher JT, Seybold K, Andres RJ, Schubiger PA, Mach JP, Buchegger F (1986) Imaging of inflammatory and infectious lesions after injection of radioiodinated monoclonal antigranulocyte antibodies. Nucl Med Commun 7:659–670
Loken MK, Clay ME, Carpenter RT, Bourdreau RJ, McCullough MC (1985) Clinical use of In-111-labelled blood products. Clin Nucl Med 10:902–911
Saverymuttu SH, Peters AM, Chadwick VJ, Hodgson HJ, Lavender JP (1981) Imaging diseased bowel with Indium-111 labelled white cells. Brit J Radiol 54:707
Sayle BA, Balachandran S, Rogers CA (1983) Indium-111 chloride imaging in patients with suspected abscesses: Concise communication. J Nucl Med 24:1114–1118
Author information
Authors and Affiliations
Additional information
Supported by the Deutsche Forschungsgemeinschaft (BE 1054/1-2)
Rights and permissions
About this article
Cite this article
Becker, W., Borst, U., Fischbach, W. et al. Kinetic data of in-vivo labeled granulocytes in humans with a murine Tc-99m-labelled monoclonal antibody. Eur J Nucl Med 15, 361–366 (1989). https://doi.org/10.1007/BF00449225
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00449225