Abstract
Two extracellular keratinases of Scopulariopsis brevicaulis were purified and partially characterized. The enzymes were isolated by the techniques of gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These keratinases (K I & K II) were purified approximately 33 and 29 fold, respectively. SDS-PAGE of the products of gel filtration chromatography (K I & II) produced only one band each, suggesting homogeneity. The optimum pH for both keratinases was 7.8, while the optimum temperatures were 40°C (K I) and 35°C (K II). Estimated molecular weights were 40–45 KDa and 24–29 KDa for K I & K II respectively. Both keratinases were inhibited by phenylmethylsulfonyl fluoride which suggests a serine residue at or near an active site.
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Malviya, H.K., Rajak, R.C. & Hasija, S.K. Purification and partial characterization of two extracellular keratinases of Scopulariopsis brevicaulis . Mycopathologia 119, 161–165 (1992). https://doi.org/10.1007/BF00448814
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DOI: https://doi.org/10.1007/BF00448814