Abstract
Trace organic nutrient and metal requirements for the growth of Methanococcoides methylutens strain TMA-10 were determined in defined medium that contained trimethylamine as the substrate. Biotin was the only organic supplement required in place of yeast extract, Trypticase or a mixture of 8 B-vitamins. Fe an Ni were required for growth and low concentrations of Fe2+ (<5 μM) and Ni2+ (<0.25 μM) provided limited growth. In the absence of added Co the growth rate was reduced by 94% and growth was limited at concentrations below 0.1 μM. Stimulation of growth by Se, Mo, B, Al, Zn, Mn or Cu could not be demonstrated.
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Sowers, K.R., Ferry, J.G. Trace metal and vitamin requirements of Methanococcoides methylutens grown with trimethylamine. Arch. Microbiol. 142, 148–151 (1985). https://doi.org/10.1007/BF00447058
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DOI: https://doi.org/10.1007/BF00447058