Summary
We report the presence of a 402 by group I intron in the chloroplast small subunit (SSU) rRNA gene of Chlamydomonas moewusii. The intron is inserted within the highly conserved ‘530 loop’, at a site corresponding to positions 531–532 of the E. coli 16rRNA. Residues surrounding the insertion site almost certainly play an important role in ribosomal proofreading function as they proved to be protected by tRNAs in E. coli 16S rRNA (Moazed and Noller 1986; Stern et al. 1986). The C. moewusii intron revealed a secondary structure model which differs substantially from those of the typical subgroup IA and IB introns. This model, however, shows striking similarities with the structures of the C. reinhardtii chloroplast 23S rRNA gene intron (Rochaix et al. 1985), the S. cerevisiae mitochondrial COB3 intron (Holl et al. 1985) and the three introns of phage T4 in the nrdB, td and sunY genes (Shub et al. 1988). The SSU rRNA gene intron is absent from C. eugametos, an alga that is interfertile with C. moewusii. The presence/absence of the intron account for a 390 by restriction fragment length polymorphism between the two algal SSU rRNA genes, a polymorphic locus that is strictly co-inherited with a tightly linked streptomycin resistance mutation (sr-2) in interspecific hybrids between the two algae.
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Biggin MD, Gibson TJ, Heng GF (1983) Proc Natl Acad Sci USA 80:3963–3965
Burger G, Scriven C, Machleidt W, Werner S (1982) EMBO J 1:1385–1391
Burke JM, Belfort M, Cech TR, Davies RW, Schweyen RJ, Shub DA, Szostak JW, Tabak HF (1987) Nucleic Acids Res 15:7217–7221
Campbell DA, Kubo K, Clark CG, Boothroyd JC (1987) J Mol Biol 196:113–124
Cech TR (1988) Gene 73:259–271
Clarke CG, Tague BW, Ware VC, Gerbi SA (1984) Nucleic Acids Res 12:6197–6220
Collins RA, Lambowitz AM (1983) Plasmid 9:53–70
Dale RM, McClure BA, Houchins JP (1985) Plasmid 13:31–40
Davies RW, Waring RB, Ray JA, Brown TA, Scazzocchio C (1982) Nature 300:719–724
Devereux J, Haeberli P, Smithies O (1984) Nucleic Acids Res 12:387–395
Dron M, Rahire M, Rochaix JD (1982) Nucleic Acids Res 10:7609–7620
Dujon B (1981) In: Strathern JN, Jones EW, Broach JR (eds) Molecular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 505–635
Erickson JM, Rahire M, Rochaix JD (1984) EMBO J 3:2753–2762
Gauthier A, Turmel M, Lemieux C (1988) Mol Gen Genet 214:192–197
Gray MW, Sankoff D, Cedergreen RJ (1984) Nucleic Acids Res 12:5837–5852
Gutell RR, Weiser B, Woese CR, Noller HF (1985) Prog Nucleic Acids Res 32:155–216
Hensgens LAM, Arnberg AC, Van der Veen R, Van Ommen GJB, Cerivell LA (1983) J Mol Biol 164:35–58
Holl J, Schmidt C, Schweyen RJ (1985) In: Quagliariello et al. (eds) Achievements and perspectives of mitochondrial research, vol II: Biogenesis. Elsevier Science, Amsterdam, pp 227–236
Jupe ER, Chapman RL, Zimmer EA (1988) BioSystems 21:223–230
Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR (1985) Proc Natl Acad Sci USA 82:6955–6959
Lemieux B, Lemieux C (1985) Curr Genet 10:213–219
Lemieux B, Turmel M, Lemieux C (1985) BioSystems 18:293–298
Lemieux C, Lee RW (1987) Proc Natl Acad Sci USA 84:4166–4170
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) Proc Natl Acad Sci USA 81:1164–1168
Lemieux C, Turmel M, Lee RW, Bellemare G (1985) Plant Mol Biol 5:77–84
Michel F, Cummings DJ (1985) Curr Genet 10:69–79
Michel F, Dujon B (1983) EMBO J 2:33–38
Michel F, Jacquier A, Dujon B (1982) Biochimie 10:867–881
Moazed D, Noller HF (1986) Cell 47:985–994
Noller HF (1984) Annu Rev Biochem 53:119–162
Norrander F, Kampe T, Messing J (1983) Gene 26:101–106
Palmer JD, Boynton JE, Gilham NW, Harris EH (1985) In: Steinbuck RE, Bonitz S, Arntzen CJ, Bogorad L (eds) Molecular biology of the photosynthetic apparatus. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 269–278
Rochaix JD, Rahire M, Michel F (1985) Nucleic Acids Res 13:975–984
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schneider M, Darlix JL, Erickson J, Rochaix JD (1985) Nucleic Acids Res 23:8531–8541
Shub DA, Gott JM, Xu MQ, Lang BF, Michel F, Tomaschewski J, Pedersen-Lane J, Belfort M (1988) Proc Natl Acad Sci USA 85:1151–1155
Spencer DF, Collings JC, Schnare MN, Gray MW (1987) EMBO J 6:1063–1071
Stern S, Wilson RC, Noller HF (1986) J Mol Biol 192:101–110
Turmel M, Bellemare G, Lemieux C (1987) Curr Genet 11:543–552
Turmel M, Lemieux B, Lemieux C (1988) Mol Gen Genet 214:412–419
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Durocher, V., Gauthier, A., Bellemare, G. et al. An optional group I intron between the chloroplast small subunit rRNA genes of Chlamydomonas moewusii and C. eugametos . Curr Genet 15, 277–282 (1989). https://doi.org/10.1007/BF00447043
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DOI: https://doi.org/10.1007/BF00447043