Summary
Tetrad analysis indicates that α-isopropylmalate synthase activity of yeast is determined by two separate genes, designated LEU4 and LEU5. LEU4 is identified as a structural gene. LEU5 either encodes another α-isopropylmalate synthase activity by itself or provides some function needed for the expression of a second structural gene. The properties of mutants affecting the biosynthesis of leucine and its regulation suggest that the expression of LEU1 and LEU2 (structural genes encoding isopropylmalate isomerase and β-isopropylmalate dehydrogenase, respectively) is controlled by a complex of a-isopropylmalate and a regulatory element (the LEU3 gene product). Similarities and differences between yeast and Neurospora crassa with respect to leucine biosynthesis are discussed.
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This is Journal Paper No. 9347 of the Agricultural Experiment Station, Purdue University
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Baichwal, V.R., Cunningham, T.S., Gatzek, P.R. et al. Leucine biosynthesis in yeast. Curr Genet 7, 369–377 (1983). https://doi.org/10.1007/BF00445877
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DOI: https://doi.org/10.1007/BF00445877