Abstract
The antibiotic tetaine inhibits in Candida albicans the biosynthesis of two important cell wall constituents, chitin and mannoprotein. This effect is a consequence of inactivation of the enzyme glucosamine-6-phosphate synthetase. Due to the lack of glucosamine-6-phosphate the effective secretion of mannoprotein enzymes, acid phosphatase and invertase, by Candida albicans spheroplasts is inhibited. In the presence of tetaine, probably a modified mannoprotein, lacking a branched polymannan, is synthesized. The antibiotic action decreases the viability of Candida albicans cells, especially that of mycelial forms of this fungus.
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Abbreviations
- GlcNAc:
-
N-acetyl-d-glucosamine
- GlcN-6-P:
-
d-glucosamine-6-phosphate
- ManNAc:
-
N-acetyl-d-mannosamine
- α-MM:
-
α-methylmannoside
- EGTA:
-
1,2 di/2-aminoethoxy/ethane
- N,N,N′,N′:
-
tetra-acetic acid
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Milewski, S., Chmara, H. & Borowski, E. Antibiotic tetaine — a selective inhibitor of chitin and mannoprotein biosynthesis in Candida albicans . Arch. Microbiol. 145, 234–240 (1986). https://doi.org/10.1007/BF00443651
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DOI: https://doi.org/10.1007/BF00443651