Abstract
A systematic study on autolysis of the cell walls of fungi has been made on Neurospora crassa, Botrytis cinerea, Polystictus versicolor, Aspergillus nidulans, Schizophyllum commune, Aspergillus niger, and Mucor mucedo. During autolysis each fungus produces the necessary lytic enzymes for its autodegradation. From autolyzed cultures of each fungus enzymatic precipitates were obtained. The degree of lysis of the cell walls, obtained from non-autolyzed mycelia, was studied by incubating these cell walls with and without a supply of their own lytic enzymes. The degree of lysis increased with the incubation time and generally was higher with a supply of lytic enzymes.
Cell walls from mycelia of different ages were obtained. A higher degree of lysis was always found, in young cell walls than in older cell walls, when exogenous lytic enzymes were present.
In all the fungi studied, there is lysis of the cell walls during autolysis. This is confirmed by the change of the cell wall structure as well as by the degree of lysis reached by the cell wall and the release of substances, principally glucose and N-acetylglucosamine in the medium.
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References
Archer, S. A. & Fielding, A. H., 1975. Thermostable polygalacturonase secreted by Sclerotinia fructigena. J. Food Sci. 40: 423–424.
Bartnicki-García, S., 1968. Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu. Rev. Microbiol. 22: 87–108.
Cooper, R. M. & Wood, R. K. S., 1975. Regulation of synthesis of cell wall degrading enzymes by Verticillum albo-atrum and Fusarium oxysporum f. sp. lycopersici. Physiol. Plant. Pathol. 5: 135–156.
Foster, J. W., 1949. Chemical nature of the mould mycelium. In: Chemical activities of fungi. Academic Press Inc. Publishers, New York, NY pp. 76–147.
Gómez, P., Reyes, F. & Lahoz, R., 1977. Effect of the level of the carbon source on the activity of some lytic enzimes released during the autolysis of Aspergillus niger. Mycopathologia 62: 23–30.
Gutcho, S. J., 1974. Fungal enzimes. In: Microbial enzyme production Chem. Tech. Rev. 28: 106–208.
Hughes, R. C., 1971. Autolysis of Bacillus cereus cell walls and isolation of structural component. Biochem. J. 121: 791–802.
Hunsley, D. & Burnett, J. H., 1970. The ultrastructural architecture of the walls of some hyphal fungi. J. Gen. Microbiol. 62: 203–218.
Ko, W. & Lockwood, J. L., 1970. Mechanism of lysis of fungal mycelia in soil. Phytopathology. 60: 148–154.
Lahoz, R., Reyes, R. & Perez-Leblic, M. I., 1977. Lytic enzymes in the autolysis of filamentous fungi. Mycopathologia. 60: 45–49.
Lindberg, B. & McPherson, J., 1954. Chemistry of lichen. VI. Pustulan. Acta Chem. Scand. 8: 985–988.
Mahadevan, P. R. & Tatum, E. L., 1967. Localization of structural polymers in the cell wall of Neurospora crassa. J. Cell. Biol. 35: 295–302.
Manocha, M. S. & Colvin, J. R., 1967. Structure and composition of the cell wall of Neurospora crassa. J. Bacteriol. 94: 202–212.
Mitchell, R. & Sabar, N., 1966. Autolytic enzymes in fungal cell walls. J. Gen. Microbiol. 42: 39–42.
Nelson, N., 1944. A photometric adaptation of the Somogyi method to determination of glucose. J. Biol. Chem. 153: 375–380.
Polachek, Y. & Rosenberger, R. F., 1975. Autolytic enzymes in hyphae of Aspergillus nidulans: their action on old and newly formed walls. J. Bacteriol. 121: 332–337.
Reese, E. T., 1977. Degradation of polymeric carbohydrates by microbial enzymes. In: The structure, biosynthesis and degradation of wood. Edited by F. Loewus and V. C. Runeckles. Plenum Press, New York. pp. 311–367.
Reyes, F., Fernandez, C. & Rodriguez, D., 1969. Aislamiento y purificatión parcial de enzimas proteoliticas de Polystictus versicolor. Resúmenes II Congreso Nacional de Microbiologia, Madrid, Spain. p. 36.
Reyes, F. & Byrde, R. J. W., 1973. Partial purification and properties of a β-N-acetylglucosaminidase from the fungus Sclerotinia fructigena. Biochem. J. 131: 381–388.
Reyes, F. & Lahoz, R., 1977. Variations in lysis of walls of Sclerotinia fructigena with age of culture. J. Gen. Microbiol. 98: 607–610.
Reyes, F., Lahoz, R. & Cornago, P., 1977. Autolysis of Neurospora crassa in different culture conditions and release of β-N-acetylglucosaminidase and chitinase. Trans. Br. Mycol. Soc. 68: 357–361.
Reyes, F., Lahoz, R. & Val Moreno, A., 1981. Synthesis of 1,3-β-glucanase and β-N-acetylglucosaminidase during autolysis of Neurospora crassa. J. Gen. Microbiol. 126: 347–353.
Santamaría, F., Reyes, F. & Lahoz, R., 1978. Extracellular glucan containing (1 → 3)-β and (1 → 6)-β linkages isolated from Monilinia fructigena. J. Gen. Microbiol. 109: 287–293.
Shibata, Y. & Nisizawa, K., 1965. Microheterogeneity of β-glucosidases in apricot emulsin. Arch. Biochem. Biophys. 109: 516–521.
Somogyi, M., 1945. A new reagent for the determination of sugars. J. Biol. Chem. 160: 61–73.
Tracey, M. V., 1955. Chitin. In: Modern methods of plant analysis. Edited by K. Paech and M. V. Tracey. Springer-Verlag, Berlin 2: 264–274.
Trevelyan, W. E. & Harrison, J. S., 1952. Studies on yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50: 298–303.
Troy, F. A. & Koffler, H., 1969. The chemistry and molecular architecture of the cell wall of Penicillium chrysogenum. J. Biol. Chem. 244: 5563–5576.
Wessels, J. G. H., 1965. Morphogenesis and biochemical processes in Schizophyllum commune. Wentia 13: 1–113.
Wessels, J. G. H. & Sietsma, J. H., 1979. Wall structure and growth in Schizophyllum commune. In: Fungal wall and hyphal growth. Edited by J. H. Burnett and A. P. J. Trinci. Cambridge University Press. Cambridge, London, New York, Melbourne. pp. 27–48.
Zonneveld, B. M., 1971. Biochemical analysis of the cell wall of Aspergillus nidulans. Biochem. Biophys. Acta 249: 506–514.
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Perez-Leblic, M.I., Reyes, F., Martinez, M.J. et al. Cell wall degradation in the autolysis of filamentous fungi. Mycopathologia 80, 147–155 (1982). https://doi.org/10.1007/BF00437577
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DOI: https://doi.org/10.1007/BF00437577