Summary
In a plastid genome (plastome) mutation of Oenothera hookeri, at least two of the plastome-coded polypeptides (the β and ε subunits) of the chloroplast ATP synthase are directly affected. As in other plastid chromosomes, the genes for the β and ε subunits are located next to each other on the Oenothera ptDNA molecule and are cotranscribed. Immunoanalysis and peptide mapping of in vivo products suggests that a fusion of the two genes may have occurred in the plastome mutant. In contrast to the in vivo data, in vitro translation of the RNA using a heterologous system results in polypeptides which cannot be distinguished from those of wild-type. In addition, neither the mRNA sizes nor plastid DNA restriction fragment patterns differ from wild-type. To reconcile the paradox of these results, it is suggested that either a defect in a translational signal or some other post-transcriptional event is responsible for the mutant phenotype.
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Alt J, Winter P, Sebald W, Moser JG, Schedel R, Westhoff P, Herrmann RG (1983) Curr Genet 7:129–138
Baltimore P (1966) J Mol Biol 18:421–428
Bünemann H (1982) Nucleic Acids Res 10:7181–7196
Bünemann H, Westhoff P, Herrmann RG (1982) Nucleic Acids Res 10:7163–7180
Cleveland DW, Fischer SW, Kirschner MW, Laemmli UK (1977) J Biol Chem 252:1102–1106
Doherty A, Gray JC (1980) Eur J Biochem 108:131–136
Ellis RJ (1977) Biochim Biophys Acta 463:185–215
Gordon KHJ, Crouse EJ, Bohnert HJ, Herrmann RG (1981) Theor Appl Genet 59:281–296
Herrmann RG, Seyer P, Schedel R, Gordon K, Bisanz C, Winter P, Hildebrandt JW, Wlaschek M, Alt J, Driesel AJ, Sears BB (1980) In: Bücher Th, Sebald W, Weiss H (eds) Biological chemistry of organelle formation. Springer, Berlin Heidelberg New York Tokyo, pp 97–112
Herrmann RG, Westhoff P, Alt J, Winter P, Tittgen J, Bisanz C, Sears BB, Nelson N, Hurt E, Hauska G, Viebrock A, Sebald W (1983) In: Ciferri O, Dure L (eds) Structure and function of plant genomes. Plenum Press, New York, pp 143–153
Howe CJ, Bowman CM, Dyer TA, Gray JC (1982) Mol Gen Genet 186:525–530
Hulla FW, Hockel M, Rack M, Risi S, Dose K (1978) Biochem 17:823–828
Krebbers ET, Larrinua IM, McIntosh L, Bogorad L (1982) Nucleic Acids Res 10:4985–5001
McMaster GK, Carmichael CG (1977) Proc Natl Acad Sci USA 74:4835–4838
Maniatis T, Fritsch EF, Sambroc J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Moroney JV, Lopresti L, McEwen BF, McCarty RE, Hammes GG (1983) FEBS Letters 158:58–62
Mendiola-Morgenthaler LR, Morgenthaler JJ, Price CA (1976) FEBS Letters 62:96–99
Merchant S, Shaner SL, Selman BR (1983) J Biol Chem 258:1026–1031
Nagata T, Takebe I (1971) Planta 99:12–20
Nelson N, Nelson H, Schatz G (1980) Proc Natl Acad Sci USA 77:1361–1364
Rigby PW, Dieckmann M, Rhodes C (1977) J Mol Biol 113:237–251
Rott R, Nelson N (1981) J Biol Chem 256:9224–9228
Sears BB, Herrmann RG (1983) J Cell Biochem 713:1318
Stubbe W, Herrmann RG (1982) In: Edelman M, Hallick R, Chua N-H (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 119–127
Thomas P (1980) Proc Natl Acad Sci USA 77:5101–5205
Towbin H, Staehelin Th, Gordon J (1979) Proc Natl Acad Sci USA 76:4350–4354
Wagenvoord R, Van der Kraan I, Kemp A (1977) Biochim Biophys Acta 460:17–24
Westhoff P, Nelson N, Bunemann H, Herrmann RG (1981) Curt Genet 4:109–120
Westhoff P, Zetsche K (1981) Eur J Biochem 116:261–267
Whitfeld PR, Zurawski G, Bottomley W (1983) In: Cifferi O, Dure L III (eds) The structure and function of plant genomes. Plenum Press, New York, pp 193–198
Zurawski G, Bottomley W, Whitfeld PR (1982) Proc Natl Acad Sci USA 79:7699–7703
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Sears, B.B., Herrmann, R.G. Plastome mutation affecting the chloroplast ATP synthase involves a post-transcriptional defect. Curr Genet 9, 521–528 (1985). https://doi.org/10.1007/BF00434057
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DOI: https://doi.org/10.1007/BF00434057