Abstract
Nisin inhibits murein synthesis with concomitant accumulation of undecaprenyl-pyrophospho-MurNAc(pentapeptide) (lipid intermediate I). This inhibition is caused by the formation of a complex between the antibiotic and lipid intermediate I. Undecaprenyl-pyrophospho-MurNAc(pentapeptide)-GlcNAc (lipid intermediate II) also forms a complex with nisin. However, when murein synthesis is inhibited by nisin, this latter complex is not formed since lipid intermediate II is no longer synthesized.
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Abbreviations
- GlcNAc:
-
N-acetylglucosamine
- MurNAc:
-
N-acetylmuramyl
- Pentapeptide:
-
Ala-γ-DGlu-Lys-DAla-DAla
- C55 :
-
undecaprenol
References
Chin, T., Younger, J., Glaser, L.: Synthesis of teichoic acids. VII. Synthesis of teichoic acids during spore germination. J. Bacteriol. 95, 2044–2050 (1968)
Dawes, J. W., Halvorson, H. O.: Membrane synthesis during outgrowth of bacterial spores. Spores V, 449–455 (1972)
Fowler, G. B., McCann, B.: The growing use of nisin in the dairy industry. Aust. J. Dairy Technol. 26, 44–46 (1971)
Hammes, W. P., Kandler, O.: Biosynthesis of peptidoglycan in Gaffkya homari. The incorporation of peptidoglycan into the cell wall and the direction of transpeptidation. Eur. J. Biochem. 70, 97–106 (1976)
Hammes, W. P., Neuhaus, F. C.: On the specificity of phospho-N-acetylmuramyl-pentapeptide translocase. The peptide subunit of uridine diphosphate-N-acetylmuramyl-pentapeptide. J. Biol. Chem. 249, 3140–3150 (1974a)
Hammes, W. P., Neuhaus, F. C.: Biosynthesis of peptidoglycan in Gaffkya homari: Role of the peptide subunit of uridine diphosphate-N-acetylmuramyl-pentapeptide. J. Bacteriol. 120, 210–218 (1974b)
Hammes, W. P., Neuhaus, F. C.: On the mechanism of action of vancomycin: Inhibition of peptidoglycan synthesis in Gaffkya homari. Antimicrob. Agents Chemother. 6, 122–128 (1974c)
Hammes, W. P., Winter, J., Kandler, O.: The sensitivity of the pseudomurein-containing genus Methanobacterium to inhibitors of murein synthesis. Arch. Microbiol. 123, 275–279 (1979)
Hembree, W. C., Ehrenkaufer, R. E., Lieberman, S., Wolf, A. P.: A general method of tritium labeling utilizing microwave discharge activation of tritium gas. Methodology and application to biological compounds. J. Biol. Chem. 248, 5532–5540 (1973)
Jordan, D. C., Reynold, P. E.: Vancomycin. In: Antibiotics: The mechanism of action, vol. 1 (Gottlieb, D., Shaw, P., eds.), pp. 102–116. Berlin, Heidelberg, New York: Springer 1967
Jarvis, B., Jeffcoat, J., Cheeseman, G. C.: Molecular weight distribution of nisin. Biochim. Biophys. Acta 168, 153–155 (1968)
Linnett, P. E., Strominger, J. L.: Additional antibiotic inhibitors of peptidoglycan synthesis. Antimicrob. Agents Chemother. 4, 231–236 (1973)
Lipinska, E.: Nisin and its application. In: Antibiotics and antibiosis in agriculture (M. Woodbine, ed.), pp. 103–130. London: Butterworth 1977
Perkins, H. R., Nieto, M.: The Chemical basis for the action of the vancomycin group of antibiotics. Ann. N.Y. Acad. Sci. 235, 348–363 (1974)
Ramseier, H. R.: Die Wirkung von Nisin auf Clostridium butyricum, Prazm. Arch. Microbiol. 37, 57–94 (1960)
Somma, S., Merati, W., Parenti, F.: Gardimycin, a new antibiotic inhibiting peptidoglycan synthesis. Antimicrob. Agents Chemother. 11, 396–401 (1977)
Struve, W. G., Sinha, R. K., Neuhaus, F. C.: On the initial stage in peptidoglycan synthesis: Phospho-N-acetylmuramyl-pentapeptide translocase (Uridine monophosphate). Biochemistry 5, 82–92 (1966)
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Dedicated to Professor Otto Kandler on occasion of his 60th birthday
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Reisinger, P., Seidel, H., Tschesche, H. et al. The effect of nisin on murein synthesis. Arch. Microbiol. 127, 187–193 (1980). https://doi.org/10.1007/BF00427192
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DOI: https://doi.org/10.1007/BF00427192