Summary
Two different PstI fragments of temperate phage ϕ105 DNA are shown to confer superinfection immunity upon Bacillus subtilis when inserted into the multicopy cloning vector pE194 cop-6. The 2.3 kb PstI fragment I is located almost entirely within EcoRI fragment F and encompasses a region previously known to encode a repressor. The other fragment, PstI-E (4.3 kb) maps inside the EcoRI-B fragment, and allows an explanation of the clear-plaque phenotype of the deletion mutant ϕ105DII:6c. The two regions can be distinguished functionally, since only the PstI fragment I product interacts with a specific ϕ105 promoter-operator site.
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Communicated by J. Schell
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Dhaese, P., Dobbelaere, MR. & Van Montagu, M. The temperate B. subtilis phage ϕ105 genome contains at least two distinct regions encoding superinfection immunity. Molec. Gen. Genet. 200, 490–492 (1985). https://doi.org/10.1007/BF00425736
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DOI: https://doi.org/10.1007/BF00425736