Abstract
Male Wistar rats were examined on the moving belt test at approximately 10, 30 and 60 min after administration of ethanol in doses ranging from 1.0–2.8 g/kg. Immediately after the test, each animal was sacrificed and ethanol concentrations were measured. The regression line of impairment as a function of brain alcohol concentration showed a progressive shift toward higher brain levels with increasing time after alcohol administration. The results confirm the existence of acute tolerance to ethanol, as defined by reduced impairment of function for a given blood level on the falling versus the rising arm of the blood alcohol curve. Confusion of interpretation due to arterio-venous differences in alcohol concentration was ruled out by simultaneous measurements in arterial blood and brain. Practice effects were ruled out by testing each animal only once.
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References
Aschan, G., Bergstedt, M., Goldberg, L., Laurell, L.: Positional nystagmus in man during and after alcohol intoxication. Quart. J. Stud. Alcohol 17, 381–405 (1956)
Casper, H., Abele, G.: Hirnelektrische Untersuchungen zur Frage der quantitativen Beziehungen zwischen Blutalkoholgehalt und Alkoholeffekt. Dtsch. Z. gerichtl. Med. 45, 492–509 (1956)
Czaja, C., Kalant, H.: The effect of acute alcoholic intoxication on adrenal ascorbic acid and cholesterol in the rat. Canad. J. Biochem. Physiol. 39, 327–334 (1961)
Eggleton, M. G.: The diuretic action of alcohol in man. J. Physiol. (Lond.) 101, 172–191 (1942)
Gibbins, R. J., Kalant, H., LeBlanc, A. E.: Technique for accurate measurement of moderate degrees of alcohol intoxication in small animals. J. Pharmacol. exp. Ther. 159, 236–242 (1968)
Goldberg, L.: Quantitative studies on alcohol tolerance in man. The influence of ethyl alcohol on sensory, motor and psychological functions referred to blood alcohol in normal and habituated individuals. Acta physiol. scand. 5, Suppl. 16, 1–128 (1943)
Gostomzyk, J. G., Dilger, B., Dilger, K.: Studies on the arterio-venous differences in blood alcohol concentration and its relationship to the alcohol content of brain. Z. klin. Chem. 7, 162–166 (1969)
Harger, R. N., Forney, R. B.: The aliphatic alcohols. In: Progress in chemical toxicology. Stolman, A., ed., vol. 1, pp. 53–134. New York: Academic Press 1963.
Heidelmann, G., Petzold, H., Taschen, G.: Untersuchung über die Nikotin- und Alkoholwirkung auf die akrale Arteriolenfunktion. Dtsch. Arch. klin. Med. 199, 431 (1952). [Abst. in Quart. J. Stud. Alcohol 14, 642–643 (1953).]
Israel, Y., Kalant, H., LeBlanc, A. E.: Effects of lower alcohols on potassium transport and microsomal adenosinetriphosphatase activity of rat cerebral cortex. Biochem. J. 100, 25–33 (1966)
Jones, B. M., Vega, A.: Cognitive performance measured on the ascending and descending limb of the blood alcohol curve. Psychopharmacologia (Berl.) 23, 99–114 (1972)
Kalant, H., LeBlanc, A. E., Gibbins, R. J.: Tolerance to, and dependence on, some non-opiate psychotropic drugs. Pharmacol. Rev. 23, 135–191 (1971)
LeBlanc, A. E.: Microdetermination of alcohol in blood by gas liquid chromatography. Canad. J. Physiol. Pharmacol. 46, 665–667 (1968)
LeBlanc, A. E., Kalant, H., Gibbins, R. J., Berman, N. D.: Acquisition and loss of tolerance to ethanol by the rat. J. Pharmacol. exp. Ther. 168, 244–250 (1969)
Maynert, E. W., Klingman, G. I.: Acute tolerance to intravenous anesthetics in dogs. J. Pharmacol. exp. Ther. 128, 192–200 (1960)
McIlwain, H.: Chemical Exploration of the Brain, p. 51. New York: Elsevier 1963.
Mellanby, E.: Alcohol: its absorption into and disappearance from the blood under different conditions. Special Report Series No. 31, Medical Research Committee, London 1919
Mirsky, I. A., Piker, P., Rosenbaum, M., Lederer, M.: “Adaptation” of the central nervous system to varying concentrations of alcohol in the blood. Quart. J. Stud. Alcohol 2, 35–45 (1941)
Story, J. L., Eidelberg, E., French, J. D.: Electrographic changes induced in cats by ethanol intoxication. Arch. Neurol. (Chic.) 5, 565–570 (1961)
Wahlström, G., Widerlöv, E.: Interaction and acute cross tolerance between ethanol and hexobarbitone in the rat. J. Pharm. Pharmacol. 23, 58–60 (1971)
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LeBlanc, A.E., Kalant, H. & Gibbins, R.J. Acute tolerance to ethanol in the rat. Psychopharmacologia 41, 43–46 (1975). https://doi.org/10.1007/BF00421304
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DOI: https://doi.org/10.1007/BF00421304