Summary
We have used cytoduction and protoplast fusion to characterise the killer trait in Torulopsis glabrata. Killer trait could not be transferred by cytoduction to a non-killer strain of T. glabrata suggesting that the determinant(s) is/are not present in the cytoplasm. Consistent with this interpretation is the observation that all fusion products, where chromosomes of both the parents are represented, are killers. Non-killer segregants could be isolated from fusion products after treatment with benlate suggesting that loss of the trait is associated with non-disjunction and loss of a chromosome.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bevan EA, Herring AJ, Mitchell DJ (1973) Nature 245:81–86
Bussey H, Skipper N (1975) J Bacteriol 124:476–483
Clark-Walker GD, Azad AA (1980) Nucleic Acids Res 8:1009–1021
Clark-Walker GD, McArthur CR, Daley DJ (1981) Curr Genet 4:7–12
Clark-Walker GD, Sriprakash KS, McArthur CR, Azad AA (1980) Curr Genet 1:209–217
Davis RW, Thomas M, Cameron J, St John TP, Scherer S, Padgett RA (1980) Methods Enzymol 65:404–411
Fink GR, Styles CA (1972) Proc Natl Acad Sci USA 69:2846–2849
Fried HM, Fink GR (1978) Proc Natl Acad Sci USA 75:4224–4228
Galeotti CL, Clark-Walker GD (1983) Changes of Gene Expression in Fusion Products between Saccharomyces cerevisiae and Kluyveromyces lactis. In: Nagley P, Linnane AW, Peacock WJ, Pateman JA (eds) Manipulation and Expression of Genes in Eukaryotes. Academic Press Australia, p 159
Galeotti CL, Sriprakash KS, Batum CM, Clark-Walker GD (1981) Mutat Res 81:155–164
Gunge N, Sakaguchi K (1981) J Bacteriol 147:155–160
Gunge N, Tamaru A, Ozawa F, Sakaguchi K (1981) J Bacteriol 145:382–390
Oakley KM, Clark-Walker GD (1978) Genetics 90:517–530
O'Connor RM, McArthur CR, Clark-Walker GD (1976) J Bacteriol 126:959–968
O'Farrell PH, Kutter E, Nakanishi M (1980) Mol Gen Genet 179:421–435
Smith GE, Summers MD (1980) Analytical Biochem 109:123–129
Sriprakash KS, Batum CM (1981) Curr Genet 4:73–80
Sweeney TK, Tate A, Fink GR (1976) Genetics 84:27–42
Upshall A, Giddings B, Mortimore ID (1977) J Gen Microbiol 100:413–418
Wesolowski M, Algeri A, Goffrini P, Fukuhara H (1982) Curr Genet 5:191–197
Wickner RB (1974) J Bacteriol 117:1356–1357
Wickner RB (1976) Bacteriol Rev 40:757–773
Young TW, Yagiu M (1978) Antonie van Leeuwenhoek 44:59–77
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sriprakash, K.S., Batum, C. Possible chromosomal location for the killer determinant in Torulopsis glabrata . Curr Genet 8, 115–119 (1984). https://doi.org/10.1007/BF00420229
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00420229