Abstract
Two new methanogenic bacteria, Methanocorpusculum sinense spec. nov. strain DSM 4274 from a pilot plant for treatment of distillery wastewater in Chengdu (Province Sichuan, China), and Methanocorpusculum bavaricum spec. nov. strain DSM 4179, from a wastewater pond of the sugar factory in Regensburg (Bavaria, FRG) are described. Methanocorpusculum strains are weakly motile and form irregularly coccoid cells, about 1 μm in diameter. The cell envelope consists of a cytoplasmic membrane and a S-layer, composed of hexagonally arranged glycoprotein subunits with molecular weights of 90000 (Methanocorpusculum parvum), 92000 (M. sinense), and 94000 (M. bavaricum). The center-to-center spacings are 14.3 nm, 15.8 nm and 16.0 nm, respectively. Optimal growth of strains is obtained in the mesophilic temperature range and at a pH around 7. Methane is produced from H2/CO2, formate, 2-propanol/CO2 and 2-butanol/CO2 by M. parvum and M. bavaricum, whereas M. sinense can only utilize H2/CO2 and formate. Growth of M. sinense and M. bavaricum is dependent on the presence of clarified rumen fluid. The G+C content of the DNA of the three strains is ranging from 47.7–53.6 mol% as determined by different methods. A similar, but distinct polar lipid pattern indicates a close relationship between the three Methanocorpusculum species. The polyamine patterns of M. parvum, M. sinense and M. bavaricum are similar, but distinct from those of other methanogens and are characterized by a high concentration of the otherwise rare 1,3-diaminopropane. Quantitative comparison of the antigenic fingerprint of members of Methanocorpusculum revealed no antigenic relationship with any one of the reference methanogens tested. On the basis of the distant phylogenetic position of M. parvum and the data presented in this paper a new family, the Methanocorpusculaceae fam. nov., is defined.
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Aldrich HC, Robinson RW, Williams DS (1986) Ultrastructure of Methanosarcina mazei. Syst Appl Microbiol 7:314–319
Aranki A, Freter R (1972) Use of anaerobic glove boxes for the cultivation of strictly anacrobic bacteria. Am J Clin Nutr 25:1329–1334
Back W, Stackebrandt E (1978) DNS/DNS-Homologiestudien innerhalb der Gattung Pediococcus. Arch Microbiol 118:79–85
Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: Reevaluation of a unique biological group. Microbiol Rev 43:260–296
Beveridge TJ, Stewart M, Doyle RJ, Sprott GD (1985) Unusual stability of the Methanospirillum hungatei sheath. J Bacteriol 162:728–737
Beveridge TJ, Harris BJ, Patel GB, Sprott GD (1986b) Cell division and filament splitting in Methanothrix concilii. Can J Microbiol 32:779–786
Beveridge TJ, Patel GB, Harris BJ, Sprott GD (1986b) The ultrastructure of Methanothrix concilii, a mesophilic aceticlastic methanogen. Can J Microbiol 32:703–710
Corder RE, Hook LA, Larkin JM, Frea JI (1983) Isolation and characterization of two new methane producing cocci: Methanogenium olentangyi, sp. nov., and Methanococcus deltae sp. nov. Arch Microbiol 134:28–32
Daniels L, Belay N, Rajagopal BS, Weimer PJ (1987) Bacterial methanogenesis and growth from CO2 with elemental iron as the sole source of electrons. Science 237:509–511
Darby GK, Jones AS, Kennedy JF, Walker RT (1970) Isolation and analysis of the nucleic acids and polysaccharides from Clostridium welchii. J Bacteriol 103:159–165
Ferguson TJ, Mah RA (1983) Isolation and characterization of an H2-oxidizing thermophilic methanogen. Appl Environ Microbiol 45:265–274
Grant WD, Pinch G, Harris JE, De Rosa M, Gambacorta A (1985) Polar lipids in methanogenic taxonomy. J Gen Microbiol 131:3177–3286
Harris JE, Pinn PA, Davis RP (1984) Isolation and characterization of a novel thermophilic, freshwater methanogen. Appl Environ Microbiol 48:1123–1128
Huber H, Thomm M, König H, Thies G, Stetter KO (1982) Methanococcus thermolithotrophicus, a novel thermophilic methanogen. Arch Microbiol 132:47–50
Huser BA, Wuhrmann K, Zehnder AJB (1982) Methanothrix soehngenii gen. nov., spec. nov., a new acetotrophic, non hydrogen oxidizing methane bacterium. Arch Microbiol 132:1–9
Jones JB, Bowers B, Stadtman TC (1977) Methanococcus vannielii: ultrastructure and sensitivity to detergents and antibiotics. J Bacteriol 130:1357–1363
Jones WJ, Leigh JA, Mayer F, Woese CR, Wolfe RS (1983) Methanococcus jannaschii sp. nov., an extremely thermophilic methanogen from a submarine hydrothermal vent. Arch Microbiol 136:254–261
Jones WJ, Nagle DP Jr., Whitman WB (1987) Methanogens and the diversity of archaebacteria. Microbiol Rev 5`:135–177
Kandler O, König H (1985) Cell envelopes of archaebacteria. In: Woese CR, Wolfe RS (eds) The Bacteria, vol. VIII. Academic Press, Inc. Orlando San Diego New York London Toronto Montreal Sydney Tokyo, pp 413–457
Kneifel H, Stetter KO, Andressen JR, Wiegel J, König H, Schoberth SM (1986) Distribution of polyamines in representative species of archaebacteria. Syst Appl Microbiol 7:241–245
König H, Stetter KO (1982) Isolation and characterization of Methanolobus tindarius, sp. nov., a coccoid methanogen growing only on methanol and methylamines. Zbl Bakt Hyg, I Abt Orig C3:478–490
König H, Stetter KO (1986) Studies on archaebacterial S-layers. Syst Appl Microbiol 7:300–309
Macario AJL, Conway de Macario E (1983) Antigenic fingerprinting of methanogenic bacteria with polyclonal antibody probes. Syst Appl Microbiol 4:451–458
Macario AJL, Conway de Macario E (1985) Monoclonal antibodies of predefined molecular specificity for identification and classification of methanogens and for probing their ecologic niches. In: Macario AJL, Conway de Macario E (eds) Monoclonal antibodies against bacteria, vol. II. Academic Press, Inc., Orlando, Florida, pp 213–247
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218
Marmur J, Doty P (1962) Determination of the base composition of desoxribonucleic acid from its thermal denaturation. J Mol Biol 5:109–118
McGill TJ, Jurka J, Sobieski JM, Pickett MH, Woese CR, Fox GE (1986) Characteristic archaebacterial 16 S rRNA oligonucleotides. Syst Appl Microbiol 7:194–197
Messner P, Hollaus F, Sleytr UB (1984) Paracrystalline cell wall surface layers of different Bacillus stearothermophilus strains. Int J Syst Bacteriol 34:202–210
Messner P, Pum D, Sára M, Stetter KO, Sleytr UB (1986) Ultrastructure of the cell envelope of the archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus. J Bacteriol 166:1046–1054
North MJ, Turner R (1976) Diamine content of the cellular slime mould Dictyostelium discoideum: presence of 1,3-diaminopropane and putrescine. Microbiol Lett 4:221–228
Ollivier BM, Mah RA, Garcia JL, Robinson R (1985) Isolation and characterization of Methanogenium aggregans sp. nov. Int J Syst Bacteriol 35:127–130
Ollivier BM, Mah RA, Garcia JL, Boone DR (1986) Isolation and characterization of Methanogenium bourgense sp. nov. Int J Syst Bacteriol 36:297–301
Paynter MJB, Hungate RE (1968) Characterisation of Methanobacterium mobilis, sp. n., isolated from the bovine rumen. J Bacteriol 95:1943–1951
Poulin R, Larochelle J, Nadeau P (1984) Polyamines in Acanthamoeba castellanii: presence of an unusually high, osmotically sensitive pool of 1,3-diaminopropane. Biochem Biophys Res Commun 122:388–392
Rivard CJ, Smith PH (1982) Isolation and characterization of a thermophilic marine methanogenic bacterium, Methanogenium thermophilicum sp. nov. Int J Syst Bacteriol 32:430–436
Rivard CJ, Henson JM, Thomas MV, Smith PH (1983) Isolation and characterization of Methanomicrobium paynteri sp. nov., a mesophilic methanogen isolated from marine sediments. Appl Environ Microbiol 46:484–490
Romesser JA, Wolfe RS, Mayer F, Spiess E, Walther-Mauruschat A (1979) Methanogenium, a genus of marine methanogenic bacteria and characterization of Methanogenium cariaci spec. nov. and Methanogenium marisnigri spec. nov. Arch Microbiol 121:147–153
Scherer P, Kneifel H (1983) Distribution of polyamines in methanogenic bacteria. J Bacteriol 154:1315–1322
Shaw PJ, Hills GJ, Henwood JA, Harris JE, Archer DB (1985) Three dimensional architecture of the cell sheath and septa of Methanospirillum hungatei. J Bacteriol 161:750–757
Sleytr UB, Messner P (1983) Crystalline surface layers on bacteria. Ann Rev Microbiol 37:311–339
Sleytr UB, Messner P, Sára M, Pum D (1986) Crystalline envelope layers in archaebacteria. Syst Appl Microbiol 7:310–313
Sleytr UB, Messner P (1988) Crystalline surface layers on bacteria. In: Sleytr UB, Messner P, Pum D, Sára M (eds) Crystalline bacterial cell surface layers. Springer, Berlin Heidelberg New York, pp 160–186
Sowers KR, Baron SF, Ferry JG (1984) Methanosarcina acetivorans sp. nov., an acetotrophic methane-producing bacterium isolated from marine sediments. Appl Environ Microbiol 47:971–978
Stackebrandt E, Ludwig W, Fox GE (1985) 16 S ribosomal RNA oligonucleotide cataloguing. In: Gottschalk G (ed) Methods in Microbiology. Academic Press, London, pp 75–107
Stetter KO, Thomm M, Winter J, Wildgruber G, Huber H, Zillig W, Janekovic D, König H, Palm P, Wunderl S (1981) Methanothermus fervidus sp. nov., a novel extremely thermophilic methanogen isolated from an icelandic hot spring. Zbl Bakt Hyg, I. Abt C2:166–178
Stewart M, Beveridge TJ, Sprott GD (1985) Crystalline order to high resolution in the sheath of Methanospirillum hungatei: A cross-beta structure. J Mol Biol 183:509–515
Tanner RS, Wolfe RS (1988) Nutritional requirements of Methanomicrobium mobile. Appl Environ Microbiol 54:625–628
Ulitzur S (1972) Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochim Biophys Acta 272:1–11
Van Brugger JJA, Zwart KB, Hermans JGF, Van Hove EM, Stumm CK, Vogels GD (1986) Isolation and characterization of Methanoplanus endosymbosium sp. nov., an endosymbiont of the marine sapropelic ciliate Metopus contortus Quennerstedt. Arch Microbiol 144:367–374
Widdel F (1986) Growth of methanogenic bacteria in pure culture with 2-propanol and other alcohols as hydrogen donors. Appl Environ Microbiol 51:1056–1062
Wiegers U, Hilz H (1971) A new method using “Proteinase K” to prevent mRNA degradation during isolation from HeLa cells. Biochem Biophys Res Commun 44:513–519
Wildgruber G, Thomm M, König H, Ober K, Ricchiuto T, Stetter KO (1982) Methanoplanus limicola, a plate-shaped methanogen representing a novel family, the Methanoplanaceae. Arch Microbiol 132:31–36
Winter J (1983) Maintenance of stock cultures of methanogens in the laboratory. Syst Appl Microbiol 4:558–563
Zabel H-P, König H, Winter J (1984) Isolation and characterization of a new coccoid methanogen, Methanogenium tatii spec. nov. from a solfataric field on Mount Tatio. Arch Microbiol 137: 308–315
Zabel H-P, König H, Winter J (1985) Emended description of Methanogenium thermophilicum, Rivard and Smith, and assignment of new isolates to this species. Syst Appl Microbiol 6:72–78
Zellner G, Winter J (1987a) Secondary alcohols as hydrogen donors for CO2-reduction by methanogens. FEMS Microbiol Lett 44:323–328
Zellner G, Winter J (1987b) Analysis of a highly efficient methanogenic consortium producing biogas from whey. Syst Appl Microbiol 9:284–292
Zellner G, Alten C, Stackebrandt E, Conway de Macario E, Winter J (1987a) Isolation and characterization of Methanocorpusculum parvum, gen. nov., spec. nov., a new tungsten requiring, coccoid methanogen. Arch Microbiol 147:13–20
Zellner G, Vogel P, Kneifel H, Winter J (1987b) Anaerobic digestion of whey and whey permeate with suspended and immobilized complex and defined consortia. Appl Microbiol Biotechnol 27:306–314
Zellner G, Bleicher K, Kneifel H, Conway de Macario E, Tindall BJ, Winter J (1989a) Isolation and characterization of a new mesophilic, secondary alcohol-utilizing methanogen, Methanobacterium palustre spec. nov., from a peat bog. Arch Microbiol 151:1–9
Zellner G, Stackebrandt E, Kneifel H, Messner P, Sleytr UB, Conway de Macario E, Zabel H-P, Stetter KO, Winter J (1989b) Isolation and characterization of a thermophilic, sulfate-reducing archaebacterium, Archaeoglobus fulgidus strain Z. Syst Appl Microbiol 11:151–160
Zillig W, Stetter KO, Wunderl S, Schulz W, Priess H, Scholz I (1980) The Sulfolobus-“Caldariella” Group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerases. Arch Microbiol 125:259–269
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Zellner, G., Stackebrandt, E., Messner, P. et al. Methanocorpusculaceae fam. nov., represented by Methanocorpusculum parvum, Methanocorpusculum sinense spec. nov. and Methanocorpusculum bavaricum spec. nov.. Arch. Microbiol. 151, 381–390 (1989). https://doi.org/10.1007/BF00416595
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DOI: https://doi.org/10.1007/BF00416595