Abstract
The extent to which a methanogen can clog sand columns was examined: two permeameters packed with clean quartz sand were sterilized, saturated with water, inoculated with Methanosarcina barkeri and percolated under upward flow conditions. After approx. 5 months, the hydraulic conductivity of the sand had decreased to 3% and 25% of the highest values measured earlier. At that point, gas-filled regions in the sand were clearly visible through the transparent walls of the permeameters, and methane bubbles were continuously released from the columns into the effluent. Scanning electron microscopy observations and biomass assays indicated that cell mass accumulation did not contribute significantly to the observed decrease of the hydraulic conductivity. This decrease was therefore attributed to pore blocking due to the entrapment of methane bubbles.
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References
Ahmad, N. 1963 The effect of evolution of gases and reducing conditions in a submerged soil on its subsequent physical status. Tropical Agriculture 40, 205–209.
Allison, L.E. 1947 Effect of microorganisms on permeability of soil under prolonged submergence. Soil Science 63, 439–450.
Blaut, M., Muller, V., Fiebig, K. & Gottschalk, G. 1985 Sodium ions and an energized membrane required by Methanosarcina barkeri for the oxidation of methanol to the level of formaldehyde. Journal of Bacteriology 164, 95–101.
Chang, A.C., Olmstead, W.R., Johanson, J.B. & Yamashita, G. 1974 The sealing mechanism of wastewater ponds. Journal of the Water Pollution Control Federation 46, 1715–1721.
Chen, R.L., Keeney, D.R., Konrad, J.G., Holding, A.J. & Graetz, D.A. 1972 Gas production in sediments of Lake Mendota, Wisconsin. Journal of Environmental Quality 1, 155–158.
Davis, S., Fairbank, W. & Weisheit, H. 1973 Dairy waste ponds effectively self-sealing. Transactions of the American Society of Agricultural Engineers 16, 69–71.
Findlay, R.H., King, G.M. & Watling, L. 1989 Efficacy of phospholipid analysis in determining microbial biomass in sediments. Applied and Environmental Microbiology 55, 2888–2893.
Ford, H.W. & Beville, B.C. 1968 Chemical changes in tile-drain filters and ditch banks caused by anaerobiosis. Transactions of the American Society of Agricultural Engineers 11, 41–42.
Ghiorse, W.C. & Wilson, J.T. 1988 Microbial ecology of the terrestrial subsurface. Advances in Applied Microbiology 33, 107–172.
Gupta, R.P. & Swartzendruber, D. 1962 Flow-associated reduction in the hydraulic conductivity of quartz sand. Soil Science Society of America, Proceedings 26, 6–10.
Gupta, R.P. & Swartzendruber, D. 1964 Entrapped air content and hydraulic conductivity of quartz sand during prolonged liquid flow. Soil Science Society of America, Proceedings 28, 9–12.
Hilton, J. & Whitehall, K.V. 1979 Investigating reduced hydraulic throughput of sand filters. Effluent and Water Treatment Journal 19, 15–25.
Jones, W.J., NagleJr, D.P. & Whitman, W.B. 1987 Methanogens and the diversity of archaebacteria. Microbiological Reviews 51, 137–177.
Jury, W.A., Gardner, W.R. & Gardner, W.H. 1991 Soil Physics. New York: John Wiley & Sons.
Kristiansen, R. 1981 Sand-filter trenches for purification of septic tank effluent: 1. The clogging mechanism and soil physical environment. Journal of Environmental Quality 10, 353–357.
Lance, J.C. & Whisler, F.D. 1972 Nitrogen balance in soil columns intermittently flooded with secondary sewage effluent. Journal of Environmental Quality 1, 180–186.
Lee, M.D., Thomas, J.M., Borden, R.C., Bedient, P.B., Wilson, J.T. & Ward, C.H. 1988 Biorestoration of aquifers contaminated with organic compounds. CRC Critical Reviews in Environmental Control 18, 29–89.
Lobo, A.L. & Zinder, S.H. 1988 Diazotrophy and nitrogenase activity in the archaebacterium Methanosarcina barkeri 227. Applied and Environmental Microbiology 54, 1656–1661.
Lovley, D.R. & Phillips, E.J.P. 1987 Competitive mechanisms for inhibition of sulfate reduction and methane production in the zone of ferric iron reduction in sediments. Applied and Environmental Microbiology 53, 2636–2641.
Mah, R.A., Smith, M.R. & Baresi, L. 1978 Studies on an acetate-fermenting Methanosarcina. Applied and Environmental Microbiology 35, 1174–1184.
Oberdorfer, J.A. & Peterson, F.L. 1985 Waste-water injection: geochemical and biogeochemical clogging processes. Ground Water 23, 753–761.
Okubo, T. & Matsumoto, J. 1979 Effect of infiltration rate on biological clogging and water quality changes during artificial recharge. Water Resources Research 15, 1536–1542.
Okubo, T. & Matsumoto, J. 1983 Biological clogging of sand and changes of organic constituents during artificial recharge. Water Research 17 813–821.
Reynolds, W.D., Brown, D.A. Mathur, S.P. & Overend, R.P. 1992 Effect of in-situ gas accumulation on the hydraulic conductivity of peat. Soil Science 153, 397–408.
Ripley, D.P. & Saleem, Z.A. 1973 Clogging in simulated glacial aquifers due to artificial recharge. Water Resources Research 9, 1047–1057.
Sanchez de Lozada, D. 1992 Reduction of the hydraulic conductivity of sand columns by Methanosarcina barkeri 227. MSc. Thesis. Cornell University, Ithaca.
Shaw, J.C., Branhill, B., Wardlaw, N.C. & Costerton, J.W. 1985 Bacterial fouling in a model core system. Applied Microbiology 49, 693–701.
Stevens, T.O., McKinley, J.P. & Fredrickson, J.K. 1993 Bacteria associated with deep, alkaline, anaerobic groundwaters in southeast Washington. Microbial Ecology 25, 35–50.
Swartzendruber, D. & Gupta, R.P. 1964 Possible role of methane in affecting the hydraulic conductivity of fine quartz sand. Soil Science 98, 73–77.
Takai, Y., Koyama, T. & Kamura, T. 1956 Microbial metabolism in reduction processes of paddy soils (Part 1). Soil Science and Plant Nutrition 2, 63–66.
Taylor, S.W., Milly, P.C.D. & Jaffe, P.R. 1990 Biofilm growth and the related changes in the physical properties of a porous medium. 2. Permeability. Water Resources Research 26, 2161–2169.
Tollner, E.W., Hill, D.T. & Busch, C.D. 1083 Manure effects on model lagoons treated with residues for bottom sealing. Transactions of the American Society of Agricultural Engineers 26, 430–435.
VanBeek, C.G.E.M. 1984 Restoring well yield in the Netherlands. Journal of the American Water Works Association 76, 66–72.
Vandevivere, P. & Baveye, P. 1992a Saturated hydraulic conductivity reduction caused by aerobic bacteria. Soil Science Society of American, Journal 56, 1–13.
Vandevivere, P. & Baveye, P. 1992b Effect of bacterial extracellular polymers on the saturated hydraulic conductivity of sand columns. Applied and Environmental Microbiology 58, 1690–1698.
Vandevivere, P. & Baveye, P. 1992c Relationship between transport of bacteria and their clogging efficiency in sand columns. Applied and Environmental Microbiology 58, 2523–2530.
Vandevivere, P. & Baveye, P. 1992d Sampling method for the observation of microorganisms in unconsolidated porous media via scanning electron microscopy. Soil Science 153, 482–485.
Vandevivere, P. & Baveye, P. 1992e Improved preservation of bacterial exopolymers for scanning electron microscopy. Journal of Microscopy 167, 323–330.
Zeikus, J.G. 1977 The biology of methanogenic bacteria. Bacteriological Reviews 41, 514–541.
Additional information
D. Sanchez de Lozada and P. Baveye are with the Department of Soil, Crop and Atmospheric Sciences, Bradfield Hall, Cornell University, Ithaca, NY 13853, USA; P. Vandevivere is with the College of Marine Studies, University of Delaware, Lewes, DE 19958, USA. S. Zinder is with the Department of Microbiology, Rice Hall, Cornell University, Ithaca, NY 14853, USA.
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Sanchez de Lozada, D., Vandevivere, P., Baveye, P. et al. Decrease of the hydraulic conductivity of sand columns by Methanosarcina barkeri . World Journal of Microbiology & Biotechnology 10, 325–333 (1994). https://doi.org/10.1007/BF00414873
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DOI: https://doi.org/10.1007/BF00414873