Summary
A lipopolysaccharide was isolated from cell walls of Anacystis nidulans by extraction with 45% aqueous phenol at 65°, and further purified by repeated high speed centrifugation. It contains 30–40% of lipid and about 60% of carbohydrate components. The carbohydrate moiety contains predominantly mannose and smaller amounts of galactose, glucose, fucose, rhamnose, 2-keto-3-deoxyoctonate, glucosamine and a second aminosugar. The latter was identified as a 2-amino-2-deoxyheptose with the gluco-configuration from C3 to C7. Thelipid moiety contains glucosamine and fatty acids (C22:0, C18:2, C16:0, C12:0 and C14:βOH). The lipopolysaccharide has a very low phosphate content and does not contain heptose. It shows low pyrogenicity in rabbits and it is not toxic in mice.
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Abbreviations
- KDO:
-
2-Keto-3-deoxy-octonate
- LPS:
-
Lipopolysaccharide
References
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Weise, G., Drews, G., Jann, B. et al. Identification and analysis of a lipopolysaccharide in cell walls of the blue-green alga Anacystis nidulans . Archiv. Mikrobiol. 71, 89–98 (1970). https://doi.org/10.1007/BF00412238
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DOI: https://doi.org/10.1007/BF00412238