Summary
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1.
A rapid decrease in the rate of CO2 fixation and nitrite oxidation was observed in autotrophically growing cultures of Nitrobacter winogradskyi.
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2.
This decline was accompanied by the accumulation of poly-β-hydroxybutyrate (PHB) and was also reflected in the decrease of the free energy efficiency of nitrite oxidation. PHB was depolymerized as soon as nitrite was depleted in the culture medium.
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3.
Besides that of PHB the synthesis of glycogen and polyphosphate by Nitrobacter winogradskyi was also demonstrated.
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References
Boon, B., Laudelout, H.: Kinetics of nitrite oxidation by Nitrobacter winogradskyi. Biochem. J. 85, 440–448 (1962).
Butler, R. G., Umbreit, W. W.: Absorption and utilization of organic matter by the strict autotroph. Thiobacillus thiooxidans, with special reference to aspartic acid. J. Bact. 91, 661–666 (1966).
——: Reduced nicotinamide adenine dinucleotide oxidase and α-ketoglutaric dehydrogenase activity by Thiobacillus thiooxidans. J. Bact. 97, 966–967 (1969).
Clark, C., Schmidt, E. L.: Growth reponse of Nitrosomonas europaea to amino acids. J. Bact. 93, 1302–1306 (1967a).
——: Uptake and utilization of amino acids by resting cells of Nitrosomonas europaea. J. Bact. 93, 1309–1315 (1967b).
Delwiche, C. C., Finstein, M. S.: Carbon and energy source for the nitrifying autotroph Nitrobacter. J. Bact. 90, 102–107 (1965).
Fischer, I., Laudelout, H.: Oxidative phosphorylation in Nitrobacter winogradskyi. Biochim. biophys. Acta (Amst.) 110, 259–264 (1965).
Fiske, C. H., Subbarow, Y.: The colorimetric determination of phosphorus. J. biol. Chem. 66, 375 (1925).
Hooper, A. B.: Biochemical basis of obligate autotrophy in Nitrosomonas europaea. J. Bact. 97, 776–779 (1969).
Ida, S., Alexander, M.: Permeability of Nitrobacter agilis to organic compounds. J. Bact. 90, 151–156 (1965).
Kelly, D. P.: Influence of amino acids and organic antimetabolites on growth and biosynthesis of the chemoautotroph Thiobacillus neapolitanus strain C. Arch. Mikrobiol. 56, 91–105 (1967).
Laudelout, H., Simonart, P. C., Van Droogenbroeck, R.: Calorimetric measurement of free energy utilization by Nitrosomonas and Nitrobacter. Arch. Mikrobiol. 63, 256–277 (1968).
London, J., Rittenberg, S. C.: Effects of organic matter on the growth of Thiobacillus intermedius. J. Bact. 91, 1062–1069 (1966).
Schön, G.: Untersuchungen über den Nutzeffekt von Nitrobacter winogradskyi Buch. Arch. Mikrobiol. 50, 111–132 (1965).
Shinn, M. B.: Colorimetric method for determination of nitrite. Ind. Engng. Chem., analyt. Ed. 13, 33–35 (1941).
Smith, A. J., Hoare, D. S.: Acetate assimilation by Nitrobacter agilis in relation to its “obligate autotrophy”. J. Bact. 95, 844–855 (1968).
— London, J. J., Stanier, R. Y.: Biochemical basis of obligate autotrophy in bluegreen algae and Thiobacilli. J. Bact. 94, 972–983 (1967).
Tobback, P., Laudelout, H.: Poly-β-hydroxybutyric acid in Nitrobacter winogradskyi. Biochim. biophys. Acta (Amst.) 97, 589–590 (1965).
Trudinger, P. A., Kelly, D. P.: Reduced nicotinamide adenine dinucleotide oxidation by Thiobacillus neapolitanus and Thiobacillus Strain C. J. Bact. 95, 1962–1963 (1963).
Tsien, H. C., Lambert, R., Laudelout, H.: Fine structure and the localization of the nitrite oxidizing system in Nitrobacter winogradskyi. Antonie v. Leeuwenhoek 34, 483–494 (1968).
Umbreit, W. W., Burris, R. H., Stauffer, J. F.: Analysis of phosphorylated intermediates. Manometric techniques. Minneapolis: Burgess Publishing Co. 1957.
Van Droogenbroeck, R., Laudelout, H.: Phosphate requirements of nitrifying bacteria. Antonie v. Leeuwenhoek 33, 483–494 (1967).
Van Gool, A. P., Lambert, R., Laudelout, H.: The fine structure of frozen etched Nitrobacter winogradskyi. Arch. Mikrobiol. 69, 281–293 (1969).
—, Laudelout, H.: Formate utilization by Nitrobacter winogradskyi. Biochim. biophys. Acta (Amst.) 127, 295–301 (1966).
——: Spectrophotometric and kinetic study of nitrite and formate oxidation in Nitrobacter winogradskyi. J. Bact. 93, 215–220 (1967).
Williams, P. J. L., Watson, S. W.: Autotrophy in Nitrocystic oceanus. J. Bact. 96, 1640–1648 (1968).
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Van Gool, A.P., Tobback, P.P. & Fischer, I. Autotrophic growth and synthesis of reserve polymers in Nitrobacter winogradskyi . Archiv. Mikrobiol. 76, 252–264 (1971). https://doi.org/10.1007/BF00409120
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DOI: https://doi.org/10.1007/BF00409120