Summary
An investigation was made of 5 species of blue-green algae reported to contain gas-vacuoles. All organisms were grown and harvested under standard conditions. Gas-vacuoles were characterised as reddish structures which are destroyed by applying pressure. Using a simple direct preparation technique gascylinders were observed with the transmission electron microscope in gas-vacuolate cells. Gas-vacuoles were present in the strains of Anabaena flos-aquae, Gloeotrichia echinulata and Oscillatoria agardhii studied and absent from Microcystis aeruginosa and Nostoc linckia. The reddish, refractile central area of N. linckia and M. aeruginosa cells was tentatively identified as nucleoplasm. Gas-vacuoles are collections of gas-cylinders 70 mμ wide, which in A. flos-aquae and G. echinulata are clearly bounded by photosynthetic lamellae and associated with α-granules. The presence of bounding photosynthetic lamellae in these species is suggested as a causal factor of the unusual optical properties of their gas-vacuoles. The range of lengths of gas-cylinders in G. echinulata and O. agardhii is from 100 mμ to 500 mμ and in A. flos-aquae it is from 100 mμ to 1300 mμ. The percentage of cell volume occupied by gas-vacuoles was estimated by direct measurement. In A. flos-aquae and G. echinulata it was 22%. In O. agardhii gas-cylinders were not clearly associated with photosynthetic lamellae and α-granules and occupied 39% of cell volume. Gascylinder membranes showed reasonable preservation in KMnO4 and excellent preservation in OsO4. The widths of membranes after treatment with these two fixatives was 3 mμ and 2 mμ respectively.
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References
Benedetti, E. L., and B. Bertolini: The use of the phosphotungstic acid (P.T.A.) as a stain for the plasma membrane. J. roy. micr. Soc. 81, 219–222 (1963).
Bowen, C. C., and T. Jensen: Blue-green algae: fine structure of the gas-vacuoles. Science 147, 1460–1462 (1965).
Canabaeus, L.: Über die Heterocysten und Gasvakuolen der Blaualgen und ihre Beziehungen zueinander. Pflanzenforsch. Bd. 13. Jena 1929.
Chapman, J., and M. Salton: A study of several blue-green algae with the electron microscope. Arch. Mikrobiol. 44, 311–322 (1962).
Fogg, G. E.: The gas-vacuoles of Myxophyceae. Biol. Rev. 16, 205–217 (1941).
Fritsch, F. E.: Structure and reproduction of the algae, Vol. 2. Cambridge 1945.
Geitler, L.: Cyanophyceae. In Rabenhorst: Kryptogamenflora, Bd. 14. Leipzig 1932.
Giesy, R. M.: Observations on the cell structure of Oscillatoria limosa Agardh. Ohio J. Sci. 62, 119–124 (1962).
—: A light and electron microscope study of interlamellar polyglucoside bodies in Oscillatoria chalybia. Amer. J. Bot. 51, 388–396 (1964).
Gorham, P. R., J. McLachlan, U. T. Hammer, and W. K. Kim: Isolation and culture of toxic strains of Anabaena flos-aquae (Lyngb.) Bréb. Verh. int. Ver. Limnol. 15, 796–804 (1964).
Gorbunova, N. P.: Gazovye vakuoli v kletkakh gormogoniev Amorphonostoc punctiforme (Kütz.) Elenk. Bot. Zhur. 45, (10), 1546–1548 (1960).
Hall, W. T., and G. Claus: The fine structure of the coccoid blue-green alga, nom. prov. Synechococcus oceanica. Rev. Biol. 5, 63–74 (1964/65).
Hopwood, D. A., and A. M. Glauert: The fine structure of the nuclear material of a blue-green alga Anabaena cylindrica. J. biophys. biochem. Cytol. 8, 813–823 (1960).
Jost, M.: Die Ultrastruktur von Oscillatoria rubescens. Arch. Mikrobiol. 50, 211–245 (1965).
—, and P. Matile: Zur Charakterisierung der Gasvacuolen der Blaualge Oscillatoria rubescens. Arch. Mikrobiol. 53, 50–58 (1966).
—, and A. Zehnder: Die Gasvakuolen der Blaualgen Microcystis aeruginosa. Schweiz. Z. Hydrol. 28, 1–3 (1966).
Klebahn, H.: Neue Untersuchungen über die Gasvacuolen. Jb. wiss. Bot. 61, 535–589 (1922).
Pankratz, H. S., and C. C. Bowen: Cytology of blue-green algae. I. The cells of Symploca muscorum. Amer. J. Bot. 50, 387–399 (1963).
Peat, A., and B. A. Whitton: Environmental effects on the structure of the blue-green alga, Chlorogloea fritschii. (In Press.)
Pringsheim, E.: The nature of pseudovacuoles in Cyanophyceae. Nature (Lond.) 210, 549–550 (1966).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Ris, H., and R. N. Singh: Electron microscope studies on blue-green algae. J. biophys. biochem. Cytol. 9, 639–680 (1961).
Robertson, J. D.: The ultrastructures of cell membranes and their derivatives. Biochem. Soc. Symp. 16, 3–43 (1959).
Sjöstrand, F. S.: A comparison of plasma membranes, cytomembranes, and mitochondrial membrane elements with respect to ultrastructural features. J. Ultrastruct. Res. 9, 561–580 (1963).
Sun, C. N.: Electron microscope observations on Gleocapsa sp. Bull. Torrey Bot. Club 88, 106–110 (1961).
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Smith, R.V., Peat, A. Comparative structure of the gas-vacuoles of blue-green algae. Archiv. Mikrobiol. 57, 111–122 (1967). https://doi.org/10.1007/BF00408695
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DOI: https://doi.org/10.1007/BF00408695