Abstract
The effect of manganese deficiency on macromolecule synthesis has been studied in a citric acid producing strain of Aspergillus niger: pulse labelling experiments showed that the synthesis of both protein and RNA was not influenced by the presence of manganese; however, increased protein degradation occurred under manganese deficiency. This was also reflected by the increased activity of an intracellular proteinase activity under these conditions. In replacement cultures addition of inhibitors of RNA, DNA or protein synthesis revealed that only emetine and cycloheximide (which both act at the ribosome) successfully antagonized the adverse effect of manganese ions on citric acid accumulation. Manganese deficiency was also characterized by a decreased portion of polysomes and 80 S ribosomes.
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Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Habison A, Kubicek CP, Röhr M (1983) Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger. Biochem J 209:669–676
Kubicek CP, Röhr M (1978) The role of the tricarboxylic acid cycle in citric acid accumulation by Aspergillus niger. Eur J Appl Microbiol Biotechnol 5:263–271
Kubicek CP, Hampel W, Röhr M (1979) Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger. Arch Microbiol 123:73–79
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Ma H (1982) Einfluß von Manganionen auf die Makromolekül-synthese in Aspergillus niger während der Citronensäuregärung. Diss Univ Wien
Orlowski ME, Sypherd PS (1978) Regulation of translation rate during morphogenesis in the fungus Mucor. Biochemistry 17:569–575
Röhr M, Stadler PJ, Salzbrunn WOJ, Kubicek CP (1979) An improved method for characterization of citrate production by conidia of Aspergillus niger. Biotechnol Letts 1:281–286
Röhr M, Kubicek CP, Kominek J (1983) Citric acid. In: Rehm HJ, Reed G (eds) Biotechnology, vol 3. Verlag Chemie, Weinheim, pp 419–454
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Ma, H., Kubicek, C.P. & Röhr, M. Metabolic effects of manganese deficiency in Aspergillus niger: evidence for increased protein degradation. Arch. Microbiol. 141, 266–268 (1985). https://doi.org/10.1007/BF00408070
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DOI: https://doi.org/10.1007/BF00408070