Abstract
The cyanobacterium Oscillatoria limnetica, capable of anoxygenic photosynthesis in the light with sulfide as electron donor can anaerobically break down its intracellular polyglucose in the dark. In the absence of elemental sulfur, the organism carries out lactate fermentation; in its presence, anaerobic respiration occurs in which sulfur is reduced to sulfide. Induction of anoxygenic photosynthesis or synthesis of new proteins is not necessary for either process. Cells adapted in the dark to sulfur reduction are capable of anoxygenic photosynthesis during a subsequent light period, unless protein synthesis has been inhibited during the dark incubation period.
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Abbreviations
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- FCCP:
-
Carbonylcyanide p-trifluoromethoxyphenylhydrazone
- mgat:
-
milligramatom
- OD:
-
optical density
References
American Public Health Association: Standard methods for the examination of water and wastewater. 13th ed., pp. 555–558. New York: American Public Health Association, Inc 1971
Breznak, J. A., Potrikus, C. J., Pfennig, N., Ensign, J. C.: Viability and endogenous substrates used during starvation survival of Rhodospirillum rubrum. J. Bacteriol. 134, 381–388 (1978)
Carr, N. G.: The occurrence of poly-β-hydroxybutyrate in the blue-green alga, Chlorogloea fritschii. Biochim. Biophys. Acta 120, 308–310 (1966)
Castenholz, R. W.: The effect of sulfide on the bluegreen algae of hot springs. I. New Zealand and Iceland. J. Phycol. 12, 54–68 (1976)
Castenholz, R. W.: The effect of sulfide on the blue-green algae of hot springs. II. Yellowstone National Park. Microb. Ecol 3, 79–105 (1977)
Cohen, Y., Jørgensen, B. B., Padan, E., Shilo, M.: Sulphide-dependent anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. Nature 257, 489–492 (1975a)
Cohen, Y., Padan, E., Shilo, M.: Faculative anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. J. Bacteriol. 123, 855–861 (1975b)
Cohen, Y., Krumbein, W. E., Shilo, M.: Solar Lake (Sinai). 2. Distribution of photosynthetic microorganisms and primary production. Limnol. Oceanogr. 22, 609–620 (1977)
Fogg, G. E., Stewart, W. D. P., Fay, P., Walsby, A. E.: The blue-green algae. London, New York: Academic Press 1973
Garlick, S., Oren, A., Padan, E.: Occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria. J. Bacteriol. 129, 623–629 (1977)
van Gemerden, H.: Growth measurements of Chromatium cultures. Arch. Mikrobiol. 64, 103–110 (1968a)
van Gemerden, H.: On the ATP generation by Chromatium in darkness. Arch. Mikrobiol. 64, 118–124 (1968b)
Gibbs, M.: Fermentation. In: Physiology and biochemistry of algae (R. A. Lewin, ed.), pp. 91–97. New York, London Academic Press 1962
Harder, W., van Dijken, J. P.: Theoretical considerations on the relation between energy production and growth of methaneutilizing bacteria. In: Microbial production and utilization of gases (H. G. Schlegel, G. Gottschalk, N. Pfennig, eds.), pp. 403–418. Göttingen: Goltze 1976
Ingvorsen, K., Jørgensen, B. B.: Combined measurement of oxygen and sulfide in water samples. Limnol. Oceanogr. (in press)
Jensen, T. E., Sicko, L. M.: Fine structure of poly-β-hydroxybutyric acid granules in a blue-green alga, Chlorogloea fritschii. J. Bacteriol. 106, 683–686 (1971)
Kondratieva, E. N.: The dark metabolism of purple sulphur bacteria. In: Proceedings of the second international symposium on photosynthetic prokaryotes (G. A. Codd, W. D. P. Stewart, eds.), pp. 47–49. Dundee 1976
Larsen, H.: On the microbiology and biochemistry of photosynthetic green sulfur bacteria. K. Nor. Vidensk. Selsk. Skr., No. 1, 199 pp. (1953)
Law, J. H., Slepecki, R. A.: Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82, 33–36 (1961)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Mackinney, G.: Absorption of light by chlorphyll solutions. J. Biol. Chem. 140, 315–322 (1941)
Madigan, M. T., Gest, H. Growth of a photosynthetic bacterium anaerobically in darkness, supported by “oxidant-dependent” sugar fermentation. Arch. Microbiol. 117, 119–122 (1978)
Nanni, G., Baldini, I.: Microdetermination of lactic acid. G. Biochim 13, 139–141 (1964)
Oren, A., Padan, E.: Induction of anaerobic, photoautotrophic growth in the cyanobacterium Oscillatoria limnetica. J. Bacteriol. 133, 558–563 (1978)
Oren, A., Padan, E., Avron, M.: Quantum yields for oxygenic and anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. Proc. nat. Acad. Sci. (Wash.) 74, 2152–2156 (1977)
Pan, P.: Growth of photoautotrophic Plectonema boryanum in the dark on glucose. Canad. J. Microbiol. 18, 275–280 (1972)
Pfennig, N., Biebl, H.: Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch. Microbiol. 110, 3–12 (1976)
Raboy, B., Padan, E., Shilo, M.: Heterophic capacities of Plectonema boryanum. Arch. Microbiol. 110, 77–85 (1976)
Roy, A. B., Trudinger, P. A.: The biochemistry of inorganic compounds of sulphur. Cambridge: University Press 1970
Sheridan, R. P.: Hydrogen sulfide production by Synechococcus lividus Y 52-s. J. Phycol. 9, 437–445 (1973)
Sheridan, R. P., Castenholz, R. W.: Production of hydrogen sulphide by a thermophilic blue-green alga. Nature 217, 1063–1064 (1968)
Schön, G., Voelskow, H.: Fermentation metabolism in Rhodospirillum rubrum adapted to anaerobic dark conditions. In: Proceedings of the second international symposium on photysynthetic prokaryotes. (G. A. Codd, W. D. P. Stewart, eds.) pp. 44–46. Dundee 1976
Trevelyan, W. E., Harrison, J. S.: Studies on yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates. Biochem. J. 50, 298–303 (1952)
Trüper, H. G., Pfennig, N.: Sulphur metabolism in Thiorhodaceae. III. Storage and turnover of thiosulphate sulphur in Thiocapsa floridana and Chromatium species. Antonie van Leeuwenhoek J. Microbiol. Serol. 32, 261–276 (1966)
White, A. W., Shilo, M.: Heterotrophic growth of the filamentous blue-green alga Plectonema boryanum. Arch. Microbiol. 102, 123–127 (1975)
Worthington Enzyme Manual Freehold (New Jersey): Worthington 1972
Yen, H. C., Marrs, B.: Growth of Rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide. Arch. Biochem. Biophys. 181, 411–418 (1977)
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Oren, A., Shilo, M. Anaerobic heterotrophic dark metabolism in the cyanobacterium Oscillatoria limnetica: Sulfur respiration and lactate fermentation. Arch. Microbiol. 122, 77–84 (1979). https://doi.org/10.1007/BF00408049
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DOI: https://doi.org/10.1007/BF00408049