Abstract
Nitrogenase from soybean bacteroids was purified and used to study NO −2 effects either as unfractionated enzyme or as reconstituted enzyme from separated nitrogenase components I and II. Partially purified enzyme was strongly inhibited by nitrite at concentrations less than 0.1 mM. This inhibition was typically referred to as competitive with an inhibition constant (K i) for NO −2 which was 5.2 mM. Kinetics studies showed an abnormally low apparent constant of association between enzyme and NO −2 (k a=60 M-1·s-1). Nitrite appeared to bind to the MoFe protein, without any effect on Fe component, giving a completely reversible inhibition. Nitrite was found not to be an alternative substrate for nitrogenase.
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Abbreviations
- TES:
-
N-tris (hydroxymethyl) methyl-2-aminoethane sulfonic acid
- PPG:
-
Polypropylene glycol
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Trinchant, J.C., Rigaud, J. Nitrite inhibition of nitrogenase from soybean bacteroids. Arch. Microbiol. 124, 49–54 (1980). https://doi.org/10.1007/BF00407027
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DOI: https://doi.org/10.1007/BF00407027