Abstract
It has previously been shown in our laboratory that wheat germ agglutinin (WGA) binds to Trichoderma viride and inhibits growth of this fungus. Here we report on the effect of WGA, soybean agglutinin (SBA) and peanut agglutinin (PNA) on Penicillia and Aspergilli. Binding of the lectins to the fungi was examined with the aid of their fluorescein isothiocyanate (FITC) conjugated derivatives. FITC-WGA bound to young hyphal walls of all species, in particular to the hyphal tips and septa, in agreement with the chitinous composition of the cell walls of the two genera. Hyphae of all species examined were labelled, though in different patterns, by FITC-SBA and FITC-PNA, suggesting the presence of galactose residues on their surfaces. Young conidiophores, metulae (of the Penicillia), vesicles (of the Aspergilli), sterigmata and young spores, were also labelled. The three lectins inhibited incorporation of [3H]acetate, N-acetyl-D-[3H]glucosamine and D-[14C]galactose into young hyphae of Aspergillus ochraceus, indicating interference with fungal growth. Inhibition of spore germination by the three lectins was also observed. Preincubation of the lectins with their specific saccharide inhibitors prevented binding and the inhibitory effects. We conclude that lectins are useful tools for the study of fungal cell surfaces, and may also serve as an important aid in fungal classification. The present findings also support the suggestion that one role of lectins in plants is protection against fungal pathogens.
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Abbreviations
- Con A:
-
concanavalin A
- PNA:
-
peanut agglutinin
- SBA:
-
soybean agglutinin
- WGA:
-
wheat germ agglutinin
- FITC:
-
fluorescein isothiocyanate
- GlcNAc:
-
N-acetyl-D-glucosamine
- GalNAc:
-
N-acetyl-D-galactosamine
References
Bardalaye, P. C., Hordin, J. H.: Galactosaminogalactan from cell walls of Aspergillus niger. J. Bacteriol. 125, 655–669 (1976)
Bartnicki-Garcia, S.: Cell wall chemistry, morphogenesis and taxonomy of fungi. Ann. Rev. Microbiol. 22, 87–107 (1968)
Clark, H. F., Shepard, C. C.: A dialysis technique for preparing fluorescent antibody. Virology 20, 642–644 (1963)
Galun, E.: Morphogenesis in Trichoderma: Autoradiography of intact colonies labelled by [3H]N-acetylglucosamine as a marker of new cell wall biosynthesis. Arch. Mikrobiol. 86, 305–314 (1972)
Galun, M., Braun, A., Frensdorff, A., Galun, E.: Hyphal walls of isolated lichen fungi. Arch. Microbiol. 108, 9–16 (1976)
Gordon, J. A., Blumberg, S., Lis, H., Sharon, N.: Purification of soybean agglutinin by affinity chromatography on Sepharose-N-ε-aminocaproyl-β-D-galactopyranosylamine. FEBS Lett. 24, 193–196 (1972)
Grisaro, V., Sharon, N., Barkai-Golan, R.: The chemical composition of the cell walls of Penicillium digitatum Sacc. and Penicilium italicum Whem. J. gen. Microbiol. 51, 145–150 (1968)
Horikoshi, K., Iida, S.: Studies of the spore coats of fungi I. Isolation and composition of the spore coats of Aspergillus oryzae. Biochim. Biophys. Acta. 83, 197–203 (1964)
Lis, H., Sharon, N.: Lectins: their chemistry and application to immunology. In: The Antigens, Vol. IV (M. Sela, ed.), pp. 429–529. New York: Academic Press 1977
Lotan, R., Gussin, A. E. S., Lis, H., Sharon, N.: Purification of wheat germ agglutinin by affinity chromatography on a Sepharose-bound N-acetylglucosamine derivative. Biochem. Biophys. Res. Commun. 52, 656–662 (1973)
Lotan, R., Skutelsky, E., Danon, D., Sharon, N.: The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J. Biol. Chem. 250, 8518–8523 (1975)
Mirelman, D., Galun, E., Sharon, N., Lotan, R.: Inhibition of fungal growth by wheat germ agglutinin. Nature 256, 414–416 (1975)
Rupley, J. A.: The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular weight substrates for lysozyme. Biochim. Biophys. Acta 83, 243–255 (1964)
Sharon, N., Lis, H.: Lectins: cell-agglutinating and sugar-specific proteins. Science 177, 949–959 (1972)
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Barkai-Golan, R., Mirelman, D. & Sharon, N. Studies on growth inhibition by lectins of penicillia and aspergilli. Arch. Microbiol. 116, 119–124 (1978). https://doi.org/10.1007/BF00406026
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DOI: https://doi.org/10.1007/BF00406026