Abstract
Twenty-nine (29) isolates (strains) were obtained from the Great Bay Estuary, New Hampshire, using a synthetic seawater medium with NaCl concentrations between 0.30 and 2.65 M. All the strains were gram-negative; did not accumulate or hydrolyze poly-β-hydroxybutyrate; demonstrated ortho ring cleavage on aromatic compounds and showed the following homogeneous characteristics: utilization of substrates, antibiotic response, acid production from sugars, colony morphology, catalase and oxidase production, lack of pigmentation and flourescence; and had a mean guanine-plus-cytosine content of deoxyribonucleic acid composition of 63.2 ± 1.1 mol %. The strains demonstrated flagellated dimorphism; being motile by a singular polar flagellum at NaCl concentrations up to 0.8 M, and nonmotile and aflagellated at higher NaCl concentrations. A total of 150 biochemical, cultural, morphological, nutritional, and physiological traits were tested for the 29 strains and the type strain of six pseudomonad reference species. The 29 strains and reference strains were then compared with other reference and nonreference strains of the following genera: Aeromonas, Alcaligenes, Alteromonas, Arthrobacter, Flavobacterium, Pseudomonas, and Vibrio using 125 unit characters and employing numerical taxonomy by the Jaccard (SJ) coefficient and single linkage clustering method. The 29 strains were similar at the 95% level and clustered with several known Pseudomonas strains between the 70–75% similarity level (S) but clustered separately at S greater than 75% level. A type strain has been deposited with the American Type Culture Collection (ATCC 29686) and has been named Pseudomonas halodurans sp. nov.
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Abbreviations
- ASWM:
-
artificial seawater medium
- BM:
-
basal medium
- BMA:
-
based medium-agar
- TTC:
-
triphenyltetrazolium chloride
- PHB:
-
poly-β-hydroxybutyrate
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Rosenberg, A. Pseudomonas halodurans sp. nov., a halotolerant bacterium. Arch. Microbiol. 136, 117–123 (1983). https://doi.org/10.1007/BF00404785
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DOI: https://doi.org/10.1007/BF00404785