Abstract
The mechanism of sulfate assimilation was investigated in Rhodopseudomonas sulfidophila, a bacterium able to grow either photoautotrophically, with sulfide as electron donor, or photoheterotrophically with sulfate as sole sulfur source. ATP sulfurylase, adenosine-5′-phosphosulfate kinase, 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase, thiosulfonate reductase and cysteine synthase were present. Reduced sulfur compounds, especially sulfide and sulfite repressed all steps of sulfate activation and reduction including sulfate uptake. Adenosine-5′-phosphosulfate kinase activity in contrast to the other activities was high in the presence of cysteine or reduced glutathione in the growth medium. Sulfur was incorporated into the cellular sulfolipid from sulfate and also from reduced sulfur compounds like cysteine and thiosulfate. The activity of 3′-phosphoadenosine-5′-phosphosulfate sulfotransferase was rapidly lost during gel filtration or dialysis. From comparison with other sulfotransferases and from the specific cofactor requirement for the enzyme of R. sulfidophila it is concluded that two different low molecular weight cofactors are required in this system. A reaction sequence is proposed involving thioredoxin as the reductant of another dialysable low molecular weight cofactor, that binds to the protein.
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Abbreviations
- APS:
-
adenosine-5′-phosphosulfate
- PAPS:
-
3′-phosphoadenosine-5′-phosphosulfate
References
Abrams WR, Schiff JA (1973) Studies of sulfate utilization by algae. 11. An enzyme-bound intermediate in the reduction of adenosine-5′-phosphosulfate (APS) by cell-free extracts of wild-type Chlorella and mutants blocked for sulfate reduction Arch Mikrobiol 94:1–10
Benson AA, Daniel H, Wiser R (1959) A sulfolipid in plants. Proc Natl Acad Sci USA 45:1582–1587
Cooper BP (1980) Der enzymatische Mechanismus der Sulfataktivierung bei Rhodospirillaceae. Doctoral thesis, University of Bonn
Hansen TA (1974) Sulfide als electronendonor voor Rhodospirillaceae. Doctoral thesis, University of Groningen
Hansen TA, Veldkamp H (1973) Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple non sulfur bacteria. Arch Mikrobiol 92:45–58
Harwood JL (1980) Sulfolipids. In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol 4. Lipid structure and function. Academic Press, New York London, pp 301–320
Hensel G, Trüper HG (1976) Cysteine and S-sulfocysteine biosynthesis in phototrophic bacteria. Arch Microbiol 109:101–103
Ibanez ML, Lindstrom ES (1962) Metabolism of sulfate by the chromatophore of Rhodospirillum rubrum. J Bacteriol 84:451–455
Imhoff JF (1982) Occurrence and evolutionary significance of two sulfate assimilation pathways in the Rhodospirillaceae. Arch Microbiol 132:197–203
Imhoff JF, Kushner DJ, Kushwaha SC, Kates M (1982) Polar lipids in phototrophic bacteria of the Rhodospirillaceae and Chromatiaceae families. J Bacteriol 150:1192–1201
Jones-Mortimer MC, Wheldrake JF, Pasternak CA (1968) The control of sulphate reduction in E. coli by O-acetylserine. Biochem J 107:51–53
Keppen OI, Gorlenko VM (1975) A new species of purple budding bacteria containing bacteriochlorophyll b. Mikrobiologiya 44:258–263
Kredich NM (1971) Regulation of l-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth on varying sulfur sources and O-acetylserine on gene expression. J Biol Chem 246:3474–3484
Neutzling O, Trüper HG (1982) Assimilatory sulfur metabolism in Rhodopseudomonas sulfoviridis. Arch Microbiol 133:145–148
Park C, Berger LR (1967) Complex lipids of Rhodomicrobium vannielii. J Bacteriol 93:221–229
Pasternak CA (1962) Sulphate activation and its control in Escherichia coli and Bacillus subtilis. Biochem J 85:44–49
Radunz A (1969) Über das Sulfochinovosyl-diacylglycerin aus höheren Pflanzen, Algen und Purpurbakterien. Hoppe-Seyler's Z Physiol Chem 350:411–417
Schmidt A (1972) On the mechanism of photosynthetic sulfate reduction. An APS-sulfotransferase from Chlorella. Arch Mikrobiol 84:77–86
Schmidt A, Christen U (1978) A factor-dependent sulfotransferase specific for 3′-phosphoadenosine-5′-phosphosulfate (PAPS) in the Cyanobacterium Synechococcus 6301. Planta 140:239–244
Schmidt A, Schwenn JD (1971) On the mechanism of photosynthetic sulfate reduction. In: Junk W (ed) Proc 2. Intern Congr Photosyn Research, Stresa, pp 507–514
Tsang MLS, Schiff JA (1976) Sulfate-reducing pathway in Escherichia coli involving bound intermediates. J Bacteriol 125:923–933
Wilson LG, Bandurski RS (1958) Enzymatic reactions involving sulfate, sulfite, selenate and molybdate. J Biol Chem 233:975–981
Wilson LG, Bierer D (1976) The formation of exchangeable sulphite from adenosine-3′-phosphate-5′-sulfatophosphate in yeast. Biochem J 158:255–270
Wilson LG, Asahi T, Bandurski RS (1961) Yeast sulfate-reducing system. I. Reduction of sulfate to sulfite. J Biol Chem 236:1822–1829
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Imhoff, J.F., Kramer, M. & Trüper, H.G. Sulfate assimilation in Rhodopseudomonas sulfidophila . Arch. Microbiol. 136, 96–101 (1983). https://doi.org/10.1007/BF00404780
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DOI: https://doi.org/10.1007/BF00404780