Abstract
Free amino acid pools have been investigated in a citric acid accumulating strain of Aspergillus niger during batch growth under manganese sufficient and deficient conditions by means of an improved chromatographic method. Studies on the mycelial content of several nitrogenous compounds under manganese sufficient and deficient conditions showed that manganese deficiency resulted in lower amino acid pool sizes during trophophase and considerable accumulation during idiophase, and in a reduction of the protein and nucleic acid contents. Addition of cycloheximide to mycelia grown with sufficient manganese also caused an elevation of free amino acid pool sizes, thus indicating that impairment of protein synthesis by manganese deficiency is responsible for the observed rise in amino acid concentration. Furthermore it was observed that the manganese deficient mycelia excreted high amounts of all amino acids suggesting that manganese deficiency may also affect membrane permeability.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Benson, J. V., Jr., Gordon, M. J., Patterson, J. A.: Accelerated chromatographic analysis of amino acids in physiological fluids containing glutamine and asparagine Anal. Biochem. 18, 228–240 (1967)
Benson, J. V., Jr., Patterson, J. A.: Accelerated chromatographic analysis of amino acids commonly found in physiological fluids by means of a spherical resin of specific design. Anal. Biochem. 13, 265–280 (1965)
Berry, D. R., Chmiel, A., Al Obaidy, Z.: Citric acid production by Aspergillus niger, in: Genetics and physiology of the Aspergillus (J. E. Smith, J. A. Pateman, eds.). London, New York, San Francisco: Academic Press 1977
Böhmer, S.-A., Leistner, H.-U., Schlee, D.: Stamm- und milieuspezifische Aminosäuremuster in Claviceps purpurea bei saprophytischer Kultur. Z. allg. Mikrobiol. 18, 233–241 (1978)
Clark, D. S., Ito, K., Horitsu, H.: Effect of manganese and other heavy metals on submerged citric acid fermentation on molasses. Biotechnol. Bioeng. 8, 465–471 (1966)
Fox, C. F., Robinson, W. S., Haselkorn, R., Weiss, S. B.: Enzymatic synthesis of ribonucleic acid: the RNA primed synthesis of RNA with Micrococcus lysodeikticus RNA-polymerase. J. Biol. Chem. 239, 175–185 (1964)
Furuya, A., Abe, S., Kinoshita, S.: Production of nucleic acid substances by fermentation processes. Part XXXI. Effects of manganese and adenine on 5′-inosinic acid accumulation by a mutant of Brevibacterium ammoniagenes. Agr. Biol. Chem. 34, 210–221 (1970)
Habison, A., Kubicek, C. P., Röhr, M.: Phosphofructokinase as a regulatory enzyme in citric acid producing Aspergillus niger. FEMS Microbiol. Lett. 5, 39–42 (1979)
Herbert, D., Phipps, P. J., Strange, R. E.: Chemical analysis of microbial cells, in: Methods in Microbiology, Vol. VB, p. 209–345 (J. R. Norris, D. W. Ribbons, eds.). London, New York: Academic Press 1971
Kimura, K.: The effect of biotin on the amino acid biosynthesis by Micrococcus glutamicus. J. gen. appl. Microbiol. 9, 205–212 (1963)
Kleber, H. P., Aurich, H.: Durch Stickstoffmangel induzierte Veränderungen der mikrobiellen Synthese von Proteinen, Nukleinsäuren und Vitaminen. Z. allg. Mikrobiol. 8, 9–18 (1968)
Kubicek, C. P., Röhr, M.: The influence of manganese on enzyme synthesis and citric acid accumulation by Aspergillus niger. Eur. J. appl. Microbiol. Biotechnol. 4, 167–175 (1977)
Kubicek, C. P., Röhr, M.: The role of the tricarboxylic acid cycle in citric acid accumulation by Aspergillus niger. Eur. J. appl. Microbiol. Biotechnol. 5, 263–271 (1978)
Kuboye, A. O., Anderson, J. G., Smith, J. E.: Control of autolysis of a spherical cell form of Aspergillus niger. Trans. Br. Mycol. Soc. 67, 27–31 (1976)
Mora, Y., Espin, G., Willms, K., Mora, J.: Nitrogen accumulation in mycelium of Neurospora crassa. J. gen. Microbiol. 104, 241–250 (1978)
Orthofer, R., Kubicek, C. P., Röhr, M.: Lipid levels and manganese deficiency in various citric acid producing strains of Aspergillus niger. FEMS Microbiol. Lett. (in press)
Reardon, J., Foreman, J. A., Leary, R. L.: New reactants for the colorimetric determination of ammonia. Clin. Chim. Acta 14, 403–410 (1966)
Röhr, M., Salzbrunn, W., Kubicek, C. P.: Studies on the mechanism of citric acid accumulation by Aspergillus niger. Proc. 5th. Int. Ferm. Symp., Berlin (H. W. Dellweg, ed.), p. 173 (1976)
Schmit, J. C., Brody, S.: Neurospora crassa conidial germination: Role of endogenous amino acid pools. J. Bacteriol. 124, 232–242 (1975)
Stevens, A., Henty, J.: Studies on the RNA-polymerase from E. coli. J. Biol. Chem. 239, 196–203 (1964)
Tellez de Inon, M. T., Leoni, P. D., Torres, H. J.: RNA polymerase activities in Neurospora crassa. FEBS Lett. 39, 91–95 (1973)
Warburg, O., Christian, W.: Remigung und Kristallisation des Gärungsfermentes Enolase. Biochem. Z. 310, 384–402 (1941)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubicek, C.P., Hampel, W. & Röhr, M. Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger . Arch. Microbiol. 123, 73–79 (1979). https://doi.org/10.1007/BF00403504
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00403504