Abstract
Two fluorescent pseudomonads, strains P17 and P500, belonging to different biotypes were tested for growth in tap water supplied with different concentrations of acetate and glutamate, low concentrations (10 and 20 μg of C per liter) of various other substrates and mixtures of related substrates, the latter being present in amounts of 1 μg of C per liter each. Amino acids appeared to be excellent substrates for both isolates, but many other substrates were utilized at very low concentrations as well. Saturation constants (Ks) of P17 with acetate, arginine, aspartate, glutamate, lactate, succinate, malonate, p-hydroxybenzoate and glucose were all below 1 μm. The Ks values of strain P500 were about 5 times larger than those of P17. Since especially P17 is able to use a large number of different substrates at low concentrations, assessment of maximal colony counts of this organism by growth experiments in various types of tap water may give information about the concentrations of easily assimilable organic carbon.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Burnison, B. K. and Morita, R. Y. 1973. Competitive inhibition for amino acid uptake by the indigenous microflora of Upper Klamath Lake.— Appl. Microbiol. 25: 103–106.
Den Dooren de Jong. L. E. 1926. Bijdrage tot de kennis van het mineralisatieproces—Thesis, Delft.
Dubler, R. E., Toscano, W. A. and Hartline, R. A. 1975. Transport of succinate by Pseudomonas putida.— Arch. Biochem. Biophys. 160: 422–429.
Eisenberg, R. C., Butters, S. J., Quay, S. C. and Friedman, S. B. 1974. Glucose uptake and phosphorylation in Pseudomonas fluorescens.— J. Bacteriol. 120: 147–153.
Hankin, L., and Sands, D. C. 1974. Bacterial production of enzymes in activated sludge systems.—J. Water Poll. Control. Fed. 46: 2015–2025.
Hoshino, T. 1979. Transport systems for branched-chain amino acids in Pseudomonas aeruginosa.— J. Bacteriol. 139: 705–712.
Kay, W. W. and Gronlund, A. F. 1969. Amino acid transport in Pseudomonas aeruginosa.— J. Bacteriol. 97: 273–281.
Kay, W. W. and Gronlund, A. F. 1971. Transport of aromatic amino acids by Pseudomonas aeruginosa.— J. Bacteriol. 105: 1039–1046.
Kay, W. W. and Kornberg, H. L. 1971. The uptake of C4-dicarboxylic acids by Escherichia coli.— Eur. J. Biochem. 18: 274–281.
Romano, A. H., Voytek, A. and Bruskin, A. M. 1980. Energization of glucose transport by Pseudomonas fluorescens.— J. Bacteriol. 142: 755–762.
Rovira, A. D. and Sands, D. A. 1971. Fluorescent pseudomonads; a residual component in the soil microflora.— J. Appl. Bacteriol. 34: 253–259.
Stanier, R. Y., Palleroni, N. J. and Doudoroff, M. 1966. The aerobic pseudomonads: a taxonomic study.— J. Gen. Microbiol. 43: 159–271.
Stinson, M. W., Cohen, M. A. and Merrick, J. M. 1976. Isolation of dicarboxylic acid and glucose-binding proteins from Pseudomonas aeruginosa.— J. Bacteriol. 28: 573–579.
Tsay, S. S., Brown, K. K. and Gaudy, A. T. 1971. Transport of glycerol by Pseudomonas aeruginosa.— J. Bacteriol. 108: 82–88.
Van der Kooij, D. 1977. The occurrence of Pseudomonas spp. in surface water and in tap water as determined on citrate media.— Antonie van Leeuwenhoek 43: 187–197.
Van der Kooij, D. 1979a. Characterization and classification of fluorescent pseudomonads isolated from tap water and surface water.— Antonie van Leeuwenhoek 45: 225–240.
Van der Kooij, D. 1979b. Ontwikkeling van een methode om de nagroeimogelijkheid van bacteriën in drinkwater te bepalen.— H2O 12: 164–167.
Van der Kooij, D. and Hijnen, W. A. M. 1981. Utilization of low concentrations of starch by a Flavobacterium species isolated from tap water.— Appl. Environ. Microbiol. 41: 216–221.
Van der Kooij, D. Visser, A. and Hijnen, W. A. M. 1980. Growth of Aeromonas hydrophila at low concentrations of substrates added to tap water.— Appl. Environ. Microbiol. 39: 1198–1204.
Windle Taylor, E. 1971–73. Silt deposits in service reservoirs. Metropolitan Water Board, Report 45: 26.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van der Kooij, D., Visser, A. & Oranje, J.P. Multiplication of fluorescent pseudomonads at low substrate concentrations in tap water. Antonie van Leeuwenhoek 48, 229–243 (1982). https://doi.org/10.1007/BF00400383
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00400383