Abstract
A new method is described for the determination of phytoplankton growth rates and carbon biomass. This procedure is easy to apply and utilizes the labeling of chlorophyll a (chl a) with 14C. Pure chl a is isolated using two-way thin-layer chromatography, and the specific activity of chl a carbon is determined. Data from laboratory cultures indicate that the specific activity of chl a carbon becomes nearly equal to that of total phytoplankton carbon in incubations lasting 6 to 12 h and can be used to calculate phytoplankton growth rates and carbon biomass. Application of the method to the phytoplankton community in an eutrophic estuary in Hawaii indicates that the cells are growing with a doubling time of about 2 d and that about 85% of the particulate carbon consists of phytoplankton carbon.
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Literature Cited
Banse, K.: Determining the carbon-to-chlorophyll ratio of natural phytoplankton. Mar. Biol. 41, 199–212 (1977)
Beers, J. R., F. M. H. Reid and G. L. Stewart: Microplankton of the North Pacific Central Gyre. Population structure and abundance. Int. Revue ges. Hydrobiol. 60, 607–638 (1975)
Bogorad, L.: Chlorophyll biosynthesis. In: Chemistry and biochemistry of plant pigments, Vol. 1, 2nd ed. pp 64–148. Ed. by T. W. Goodwin. New York: Academic Press 1976
Calvin, M., J. A. Bassham, A. A. Benson, V. H. Lynch, C. Quellet, L. Shou, W. Stepka and N. E. Tolbert: Carbon dioxide assimilation in plants. Symp. Soc. exp. Biol. 5, 284–305 (1951)
Caperon, J. and J. Meyer: Nitrogen-limited growth of marine phytoplankton. I. Changes in population characteristics with steady-state growth rate. Deep-Sea Res. 19, 601–618 (1972)
Derenbach, J. B. and P. J. LeB. Williams: Autotrophic and bacterial production: fractionation of plankton populations by differential filtration of samples from the English Channel. Mar. Biol. 25, 263–269 (1974)
Eppley, R. W.: An incubation method for estimating the carbon content of phytoplankton in natural samples. Limnol. Oceanogr. 13, 574–582 (1968)
Eppley, R. W., A. F. Carlucci, O. Holm-Hansen, D. Kiefer, J. J. McCarthy, E. L. Venrick and P. M. Williams: Phytoplankton growth and composition in shipboard cultures supplied with nitrate, ammonium, or urea as a nitrogen source. Limnol. Oceanogr. 16, 741–751 (1971)
Eppley, R. W., A. F. Carlucci, O. Holm-Hansen, D. Kiefer, J. J. McCarthy and P. M. Williams: Evidence for eutrophication in the sea near southern California coastal sewage outfalls-July, 1970. Rep. Calif. coop. ocean. Fish. Invest (CalCOFI Rep.) 16, 74–83 (1972)
Eppley, R. W., W. G. Harrison, S. W. Chisholm and E. Stewart: Particulate organic matter in surface waters off Southern California and its relationship to phytoplankton. J. mar. Res. 35, 671–695 (1977)
Eppley, R. W. and E. H. Renger: Nitrogen assimilation of an oceanic diatom in nitrogen-limited continuous culture. J. Phycol. 10, 15–23 (1974)
Eppley, R. W., E. H. Renger, E. L. Venrick and M. M. Mullin: A study of plankton dynamics and nutrient cycling in the central gyre of the North Pacific Ocean. Limnol. Oceanogr. 18, 534–551 (1973)
Gieskes, W. W. C., G. W. Kraay and M. A. Baars: Current 14C methods for measuring primary production: gross underestimates in oceanic waters. Neth. J. Sea Res. 13, 58–78 (1979)
Goldman, J. C. and E. J. Carpenter: A kinetic approach to the effect of temperature on algal growth. Limnol. Oceanogr. 19, 756–766 (1974)
Goldman, J. C., J. J. McCarthy and D. G. Peavey: Growth rate influence on the chemical composition of phytoplankton in oceanic waters. Nature, Lond. 279, 210–215 (1979)
Grumbach, K. H., H. K. Lichtenthaler and K. H. Erismann: Incorporation of 14CO2 in photosynthetic pigments of Chlorella pyrenoidosa. Planta 140, 37–43 (1978)
Hitchcock, G. L.: The time course of photosynthetic adaptation, the growth response and variation in pigment, carbohydrate, and protein of Skeletonema costatum and Detonula confervacea to changes in light intensity, Ph. D. thesis, University of Rhode Island, Narragansett, Rhode Island 1977
Holm-Hansen, O. and C. R. Booth: The measurement of ATP in the ocean and its ecological significance. Limnol. Oceanogr. 11, 510–519 (1966)
Hunter, B. L.: ATP and chlorophyll a as estimators of phytoplankton metabolic activity and carbon biomass, 77 pp. M.S. thesis, University of Hawaii 1979
Jeffrey, S. W.: Profiles of photosynthetic pigments in the ocean using thin-layer chromatography. Mar. Biol. 26, 101–110 (1974)
Jeffrey, S. W. and G. F. Humphrey: New spectrophotometric equations for determining chlorophylls a,b,c and c 2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pfl. 167, 191–194 (1975)
Laws, E. A. and T. T. Bannister: Nutrient- and light-limited growth of Thalassiosira fluviatilis in continuous culture, with implications for phytoplankton growth in the ocean. Limnol. Oceanogr. 25, 457–473 (1980)
Laws, E. A. and D. C. L. Wong: Studies of carbon and nitrogen metabolism by three marine phytoplankton species in nitrate-limited continuous culture. J. Phycol. 14, 406–416 (1978)
Lean, D. R. S. and B. K. Burnison: An evaluation of errors in the 14C method of primary production measurement. Limnol. Oceanogr. 24, 917–938 (1979)
Li, W. K., H. E. Glover and I. Morris: Physiology of carbon photoassimilation by Oscillatoria thiebautii. Limnol. Oceanogr. 25, 447–456 (1980)
Perry, M. J.: Phosphate utilization by an oceanic diatom in phosphorus-limited chemostat culture and in the oligotrophic waters of the central North Pacific. Limnol. Oceanogr. 21, 88–107 (1976)
Redalje, D. G.: The effects of environmental factors on the general patterns of carbon metabolism for marine phytoplankton, 136 pp. Ph.D. dissertation, University of Hawaii 1980
Riper, D. M., T. G. Owens and P. G. Falkowsky: Chlorophyll turnover in Skeletonema costatum, a marine plankton diatom. Pl. Physiol., Lancaster 64, 49–54 (1979)
Saino, T. and A. Hattori: Estimate of the growth rate of phytoplankton in the surface waters of the Bering Sea and the Northern North Pacific. Mar. Sci. Communs 3, 1–19 (1977)
Schindler, D. W., R. V. Schmidt and R. A. Reid: Acidification and bubbling as an alternative to filtration in determining phytoplankton production by the 14C method. J. Fish. Res. Bd Can. 29, 1627–1631 (1972)
Sharp, J. H.: Improved analysis for “particulate” organic carbon and nitrogen from seawater. Limnol. Oceanogr. 19, 984–989 (1974)
Sheldon, R. W. and W. H. Sutcliffe, Jr.: Generation time of 3 h for Sargasso Sea microplankton determined by ATP analysis. Limnol. Oceanogr. 23, 1051–1055 (1978)
Sheldon, R. W., W. H. Sutcliffe, Jr. and A. Prakash: The production of particles in the surface waters of the ocean with particular reference to the Sargasso Sea. Limnol. Oceanogr. 18, 719–733 (1973)
Sinclair, M., E. Keighan and J. Jones: ATP as a measure of living phytoplankton carbon in estuaries. J. Fish. Res. Bd Can. 36, 180–186 (1979)
Smith, W. O. and R. T. Barber: A carbon budget for the autotrophic ciliate Mesodinium rubrum. J. Phycol. 15, 27–33 (1979)
Steele, J. H. and I. E. Baird: Further relations between primary production, chlorophyll and particulate carbon. Limnol. Oceanogr. 6, 68–78 (1961)
Steemann Nielsen, E.: The use of radioactive carbon (14C) for measuring organic production in the sea. J. Cons. perm. int. Explor. Mer 18, 117–140 (1952)
Steemann Nielsen, E. and V. K. Hansen: Light adaptation in marine phytoplankton populations and its interrelation with temperature. Physiologia Pl. 12, 353–370 (1959)
Strathmann, R. R.: Estimating the organic carbon content of phytoplankton from cell volume or plasma volume. Limnol. Oceanogr. 12, 411–418 (1967)
Strickland, J. D. H. and T. R. Parsons: A practical handbook of seawater analysis, 2nd ed. Bull. Fish. Res. Bd. Can 167, 1–310 (1972)
Sutcliffe, W. H. Jr., R. W. Sheldon and A. Prakash: Certain aspects of production and standing stock of particulate matter in the surface waters of the northwest Atlantic Ocean. J. Fish. Res. Bd Can. 27, 1917–1926 (1970)
Swift, E. and E. G. Durbin: The phased division and cytological characteristics of Pyrocystis spp. can be used to estimate doubling times of their populations in the sea. Deep-Sea Res. 19, 189–198 (1972)
Weiler, C. S.: Population structure and in situ division rates of Ceratium in oligotrophic waters of the North Pacific Central Gyre. Limnol. Oceanogr. 25, 610–619 (1980)
Weiler, C. S. and S. W. Chisholm: Phased cell division in natural populations of marine dinoflagellates from shipboard cultures. J. exp. mar. Biol. Ecol. 25, 239–247 (1976)
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Communicated by N. D. Holland, La Jolla
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Redalje, D.G., Laws, E.A. A new method for estimating phytoplankton growth rates and carbon biomass. Mar. Biol. 62, 73–79 (1981). https://doi.org/10.1007/BF00396953
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DOI: https://doi.org/10.1007/BF00396953