Summary
Following screening, selection, characterization, and symbiotic N2 fixation with 12,5, 25.0, and 40.0 mg N kg−1 in normal and saline-sodic soils, only two Phaseolus vulgaris genotypes (HUR 137 and VL 63) and two Rhizobium spp. strains (ND 1 and ND 2) produced maximum nodulation, nitrogenase activity, plant N contents, and grain yields in saline-sodic soil, with 12.5 mg N kg−1, compared with the other strains. However, interactions between strains (USDA 2689, USDA 2674, and ND 5) and genotypes (PDR 14, HUR 15, and HUR 138) were significant and resulted in more nodulation, and greater plant N contents, nitrogenase activity, and grain yields in normal soils with 12.5 mg N kg−1 compared with salt-tolerant strains. Higher levels of N inhibited nodulation and nitrogenase activity without affecting grain yields. To achieve high crop yields from saline-sodic and normal soils in the plains area, simultaneous selection of favourably interacting symbionts is necessary for N economy, so that bean yields can be increased by the application of an active symbiotic system.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Doque FF, Neves MCP, Franco AA, Victoria RL, Boddey RM (1985) The response of field grown Phaseolus vulgaris to Rhizobium inoculation and quantification of N2 fixation using 15N. Plant and Soil 88:333–343
Hartle PC, Alexander M (1983) Growth and survival of cowpea rhizobia in acid, aluminium-rich soil. Soil Sci Soc Am J 47:502–506
Jackson ML (1978) Soil chemical analysis. Prentice-Hall, New Delhi, India
Liao CFH (1981) Devarde's alloy method for total nitrogen determination. Soil Sci Soc Am J 45:852–855
Neves MCP, Didonet AD, Doque FF, Döbereiner J (1985) Rhizobium strain effects of nitrogen transport and distribution on soybeans. J Exp Bot 36:1179–1192
Rai R (1983) The salt tolerance of Rhizobium strains and lentil genotypes and the effect of salinity on aspects of symbiotic N-fixation. J Agric Sci 100:81–88
Rai R (1987) Chemotaxis of salt-tolerant and sentitive Rhizobium strains to root exudates of lentil (Lens culinaris L.) genotypes and symbiotic N-fixation, prolene content and grain yield in saline calcareous soil. J Agric Sci 108:25–37
Rai R, Prasad V (1983) Salinity tolerance of Rhizobium mutants: Growth and relative efficiency of symbiotic nitrogen fixation. Soil Biol Biochem 15:215–217
Rai R, Singh SN (1979) Response of Rhizobium strains on nodulation, grain yield, protein and amino acids content of chickpea (Cicer arietinum L.). J Agric Sci 93:47–49
Rai R, Nasar SKT, Singh SJ, Prasad V (1985) Interaction between Rhizobium strains and lentil (Lens culinaris L.) genotypes under saltstress. J Agtric Sci 104:199–205
Rennie RJ, Kemp GA (1983) N2 fixation in field beans quantified by N15 isotope dilution: 2. Effect of cultivars of bean. Agron J 75:645–649
Vincent JM (1970) A manual for the practical study of the root nodule bacteria. International Biological Programme Handbook 15, Blackwell, Oxford
Westermann DT, Porter LK, O'Deen WA (1985) Nitrogen partitioning and mobilization patterns in bean plants. Crop Sci 25:225–229
Zeiher C, Egle DB. Leggett JE, Reicosky DA (1982) Cultivars differences in N redistribution in soybeans. Agron J 74:375–379
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rai, R. Effect of nitrogen levels and Rhizobium strains on symbiotic N2 fixation and grain yield of Phaseolus vulgaris L. genotypes in normal and saline-sodic soils. Biol Fert Soils 14, 293–299 (1992). https://doi.org/10.1007/BF00395466
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00395466