Abstract
The lipid compositions of leaves from Festuca pratensis cv. Rossa (yellowing) were compared with those from a non-yellowing mutant, Bf 993. The leaves of Bf 993 contained a higher level of acyl lipids on both a fresh-weight and a dry-weight basis. Diacylgalactosylglycerol, diacylgalabiosylglycerol and phosphatidylinositol were relatively enriched in the Bf 993 mutant while phosphatidylcholine was relatively reduced. There were no differences in the fatty-acid compositions of individual lipids between the two varieties. During senescence, the lipids of cv. Rossa were progressively degraded over an 8-d period. In contrast little lipid degradation was observed in the Bf 993 mutant during the first 4 d. The results support the hypothesis that the slower senescence changes of the Bf 993 mutant may be due, in part, to an altered membrane lipid composition.
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Anderson, J.M. (1975) The molecular organisation of chloroplast thylakoids. Biochim. Biophys. Acta 416, 191–235
Burns, D.D., Galliard, T., Harwood, J.L. (1979) Purification of acyl hydrolase enzymes from the leaves of Phaseolus multiflorus. Phytochemistry 18, 1793–1797
Carter, D.P., Staehelin, L.A. (1980) Proteolysis of chloroplast thylakoid membranes. I. Selective degradation of thylakoid pigment-protein complexes at the outer membrane surface. Arch. Biochem. Biophys. 200, 364–373
Downing, D.T., Greene, K.S. (1968) Rapid determination of double bond positions in monoenoic fatty acids by potassium permanganate oxidation. Lipids 3, 96–100
Fong, F., Heath, R.L. (1977) Age dependent changes in phospholipids and galactolipids in primary bean leaves (Phaseolus vulgaris). Phytochemistry 16, 215–217
Garbus, J., De Luca, H.F., Loomans, M.E., Strong, F.M. (1963) The rapid incorporation of phosphate into mitochondrial lipids. J. Biol. Chem. 238, 59–63
Harwood, J.L. (1976) Synthesis of molecular species of phosphatidylcholine and phosphatidylethanolamine by germinating soya bean. Phytochemistry 15, 1459–1463
Harwood, J.L. (1980) Plant acyl lipids. In: Biochemistry of plants, vol. 4, pp. 1–55, Stumpf, P.K., Conn, E.E., eds. Academic Press, New York London
Heinz, E., Harwood, J.L. (1977) Incorporation of carbon dioxide, acetate and sulphate into the glycerolipids of Vicia faba leaves. Hoppe-Seylers Z. Physiol. Chem. 358, 897–908
Heinz, E., Siefermann-Harms, D. (1981) Are galactolipids integral components of the chlorophyll-protein complexes in spinach thylakoids? FEBS Lett. 124, 105–111
Huffaker, R.C., Miller, B.L. (1978) Reutilization of ribulose bisphosphate carboxylase. In: Photosynthetic carbon assimilation, pp. 139–152, Siegelman, H.W., Hind, G., eds. Plenum, New York
James, W.O. (1953) Plant respiration. Oxford University Press, Oxford
Khan, M.-U., Williams, J.P. (1977) Improved thin-layer chromatographic method for the separation of major phospholipids and glycolipids from plant lipid extracts and phosphatidylglycerol and bis (monoacylglyceryl) phosphate from animal lipid extracts. J. Chromatogr. 140, 179–185
Koiwai, A., Matsuzaki, T., Suzuki, F., Kawashima, N. (1981) Changes in total and polar lipids and their fatty acid composition in tobacco leaves during growth and senescence. Plant Cell Physiol. 22, 1059–1065
Lloyd, E.J. (1980) The effects of leaf age and senescence on the distribution of carbon in Lolium temulentum. J. Exp. Bot. 31, 1067–1079
Lloyd, E.J. (1982) Mobilization of carbon during senescence in detached leaves of Lolium temulentum. Plant Sci. Lett. 25, 313–319
Mazliak, P., Benveniste, P., Costes, C., Douce, R., eds. (1980) Biogenesis and function of plant lipids. Elsevier, Amsterdam
Meyer, H.-U., Biehl, B. (1981) Activation of latent phenolase during spinach leaf senescence. Phytochemistry 20, 955–959
Pollock, C.J., Lloyd, E.J. (1978) Acid invertase activity during senescence of excised leaf tissue of Lolium temulentum. Z. Pflanzenphysiol. 90, 79–84
Rawyler, A., Henry, L., Siegenthaler, P.-A. (1980) Acyl and pigment lipid composition of two chlorphyll-proteins. Carlsberg Res. Commun. 45, 443–451
Roehm, J.N., Privett, O.S. (1969) Improved method for the determination of the positions of double bonds in polyenoic fatty acid ester. J. Lipid Res. 10, 245–246
Thomas, H. (1976) Delayed senescence in leaves treated with the protein synthesis inhibitor MDMP. Plant Sci. Lett. 6, 369–377
Thomas, H. (1977) Ultrastructure, polypeptide composition and photochemical activity of chloroplasts during foliar senescence of a non-yellowing mutant genotype of Festuca pratensis Huds. Planta 137, 53–60
Thomas, H. (1982a) Leaf senescence in a non-yellowing mutant of Festuca pratensis. I. Chloroplast membrane polypeptides. Planta 154, 212–218
Thomas, H. (1982b) Leaf senescence in a non-yellowing mutant of Festuca pratensis. II. Proteolytic degradation of thylakoid and stroma polypeptides. Planta 154, 219–223
Thomas, H., Stoddart, J.L. (1975) Separation of chlorophyll degradation from other senescence processes in leaves of a mutant genotype of meadow fescue (Festuca pratensis). Plant Physiol. 56, 438–441
Thomas, H., Stoddart, J.L. (1980) Leaf senescence. Annu Rev. Plant Physiol. 31, 83–111
Unitt, M.D., Harwood, J.L. (1982) Investigation of the transmembrane distribution of thylakoid lipids. Biochem. Soc. Trans. 10, 249–250
Wharfe, J., Harwood, J.L. (1978) Fatty acid biosynthesis in the leaves of barley, wheat and pea. Biochem. J. 174, 163–169
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Harwood, J.L., Jones, A.V.H.M. & Thomas, H. Leaf senescence in a non-yellowing mutant of Festuca pratensis . Planta 156, 152–157 (1982). https://doi.org/10.1007/BF00395429
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DOI: https://doi.org/10.1007/BF00395429