Abstract
A panel of twelve monoclonal antibodies (MAbs), designated FS1 to FS12, have been raised against surface antigens of Fucus serratus sperm. The antibodies were selected on the basis that they show region-, gamete-, species- or genus-preferential binding. Indirect immunofluorescence shows that the antigens bound by the MAbs are distributed non-randomly over the cell surface. Seven MAbs (FS1, FS3, FS4, FS6, FS8, FS9, FS10) bind antigens located primarily on the cell body, while the others (FS2, FS5, FS7, FS11, FS12) bind antigens located primarily on the anterior flagellum. Of the MAbs that label the anterior flagellum, FS2, FS5, FS7 and FS12 form a ‘halo’ at the perimeter of the flagellum. Electron microscopic-immunogold studies indicate that the ‘halo’ results from labelling of the mastigonemes, as opposed to the flagellar plasmamembrane. Gamete-preferential binding of antibodies was detected using an enzyme-linked immunosorbent assay with egg membrane vesicles. Eight of the MAbs bind sperm antigens not common to eggs, though FS2, FS4, FS5 and FS9 bind antigens present on both sperm and eggs. In studies of species- and genus-specificity FS2, FS3, FS5, FS6, FS7, FS8, FS10, FS11 and FS12 exhibit genus-preferential binding, labelling sperm of F. serratus and F. vesiculosus more intensely than that of Ascophyllum nodosum. Only FS10 showed marked species-preferential binding, labelling sperm of F. serratus much more intensely than that of F. vesiculosus.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Au-GAMIG:
-
gold-conjugated goat anti-mouse immunoglobulin
- ELISA:
-
enzyme-linked immunosorbent assay
- EM:
-
electron microscope
- FITC-RAMIG:
-
fluorescein-isothiocyanate-conjugated rabbit anti-mouse immunoglobulin
- IIF:
-
indirect immunofluorescence
- MAb:
-
monoclonal antibody
References
Bolwell, G.P., Callow, J.A., Callow, M.E., Evans, L.V. (1979) Fertilisation in brown algae. II. Evidence for lectin-sensitive complementary receptors involved in gamete recognition in Fucus serratus. J. Cell Sci. 36, 19–30
Bolwell, G.P., Callow, J.A., Evans, L.V. (1980) Fertilisation in brown algae. III. Preliminary characterisation of putative gamete receptors from eggs and sperm of Fucus serratus. J. Cell Sci. 43, 209–224
Bouck, G.B. (1969) Extracellular microtubules: The origin, structure and attachment of flagellar hairs in Fucus and Ascophyllum antherozoids. J. Cell Biol. 40, 446–460
Callow, J.A. (1985) Sexual recognition and fertilisation in brown algae. J. Cell Sci. Suppl. 2, 219–232
Callow, M.E., Evans, L.V., Bolwell, G.P., Callow, J.A. (1978) Fertilisation in brown algae. I. SEM and other observations on Fucus serratus. J. Cell Sci. 32, 45–54
Catt, J.W., Vithanage, H.I.M.V., Callow, J.A., Callow, M.E., Evans, L.V. (1983) Fertilisation in brown algae. V. Further investigations of lectins as surface probes. Exp. Cell Res. 147, 127–133
Cowan, A.E., Myles, D.G., Koppel, D.E. (1987) Lateral diffusion of the PH-20 protein on guinea pig sperm: Evidence that barriers to diffusion maintain plasma membrane domains in mammalian sperm. J. Cell Biol. 104, 917–923
Crothers, J.H. (1976) On the distribution of some common animals and plants along the rocky shores of West Somerset. Field Studies 4, 369–389
Galfre, G., Milstein, C. (1981) Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 73, 1–46
Gaunt, S.J., Brown, C.R., Jones, R. (1983) Identification of mobile and fixed antigens on the plasmamembrane of rat spermatozoa using monoclonal antibodies. Exp. Cell Res. 144, 275–284
Gumbiner, B., Louvard, D. (1985) Localized barriers in the plasma membrane: a common way to form domains. Trends Biochem. Sci. 10, 435–438
Holt, W.V. (1985) Membrane heterogeneity in the mammalian spermatozoon. Int. Rev. Cytol. 87, 159–194
Manton, I., Clark, B. (1951) An electron microscope study of the spermatozoid of Fucus serratus. Ann. Bot. 60, 461–471
Marc, J., Gunning, B.E.S., Hardham, A.R., Perkin, J.L., Wick, S.M. (1988) Monoclonal antibodies to surface and cytoskeletal components of the spermatozoid of Pteridium aquilinum. Protoplasma 142, 5–14
Muller, D.G., Seferiadis, K. (1977) Specificity of sexual chemotaxis in Fucus serratus and Fucus vesiculosus (Phaeophyceae). Z. Pflanzenphysiol. 84, 85–94
Musgrave, A., de Wildt, P., van Etten, I., Pijst, C., Scholma, C., Kooyaman, R., Homan, W., van den Ende, H. (1986) Evidence for a functional membrane barrier in the transition zone between the flagellum and cell body of Chlamydomonas eugametos gametes. Planta 167, 544–553
Primakoff, P., Hyatt, H., Tredick-Kline, J. (1987) Identification and purification of a sperm surface protein with a potential role in sperm-egg membrane fusion. J. Cell Biol. 104, 141–149
Primakoff, P., Myles, D.G. (1983) A map of the guinea pig sperm surface constructed with monoclonal antibodies. Dev. Biol. 98, 417–428
Saling, P.M. (1986) Mouse sperm antigens that participate in fertilisation. IV. A monoclonal antibody prevents zona penetration by inhibition of the acrosome reaction. Dev. Biol. 117, 511–519
Saling, P.M., Irons, G., Waibel, R. (1985) Mouse sperm antigens that participate in fertilisation. I. Inhibition of sperm fusion with the egg plasma membrane using monoclonal antibodies. Biol. Reprod. 33, 515–526
Saling, P.M., Lakoski, K.A. (1985) Mouse sperm antigens that participate in fertilisation. II. Inhibition of sperm penetration through the zona pellucida using monoclonal antibodies. Biol. Reprod. 33, 527–536
Saxena, N.K., Russell, L.D., Saxena, N., Peterson, R.N. (1986) Immunofluorescence antigen localisation on boar sperm plasma membranes: monoclonal antibodies reveal apparent new domains and apparent redistribution of surface antigens during sperm maturation and at ejaculation. Anat. Rec. 214, 238–252
Vithanage, H.I.M.V., Catt, J.W., Callow, J.A., Callow, M.E., Evans, L.V. (1983) Fertilisation in brown algae. IV. Appearance of sperm-specific antigens on fertilised eggs. J. Cell Sci. 60, 103–108
Williams, A.F. (1980) Cell surface antigens of lymphocytes: markers and molecules. Biochem. Soc. Symp. 45, 27–50
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jones, J.L., Callow, J.A. & Green, J.R. Monoclonal antibodies to sperm surface antigens of the brown alga Fucus serratus exhibit region-, gamete-, species- and genus-preferential binding. Planta 176, 298–306 (1988). https://doi.org/10.1007/BF00395410
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00395410