Abstract
A cell-free extract from flowers of Matthiola incana catalyzes a NADPH-dependent stereospecific reduction of (+)-dihydrokaempferol to 3,4-cis-leucopelargonidin (5,7,4′-trihydroxyflavan-3,4-cis-diol). The pH-optimum of this reaction is around 6. The rate of reaction with NADH was about 50% of that found with NADPH. (+)-Dihydroquercetin and (+)-dihydromyricetin were also reduced by the enzyme preparation to the corresponding flavan-3,4-cis-diols. Correlation between the genotype of M. incana and the presence of dihydroflavonol 4-reductase is strong evidence that this enzyme is involved in anthocyanin biosynthesis.
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References
Barton, G.M. (1968) Detection of 3-hydroxy flavanones on papergrams and thin-layer plates. J. Chromatogr. 34, 562
Bensadoun, A., Weinstein, B. (1976) Assay of proteins in the presence of interfering materials. Anal. Biochem. 70, 241–250
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248–254
Britsch, L., Heller, W., Grisebach, H. (1981) Conversion of flavanone to flavone, dihydroflavonol and flavons with an enzyme system from cell cultures of parsley. Z. Naturforsch. Teil C. 36, 742–750
Dangelmayr, B., Stotz, G., Spribille, R., Forkmann, G. (1983) Relationship between flower development, anthocyanin accumulation and activity of enzymes involved in flavonoid biosynthesis in Matthiola incana R. Br. Z. Naturforsch. Teil C. 38, 551–555
Forkmann, G. (1977) Precursors and genetic control of anthocyanin synthesis in Matthiola incana R. Br. Planta 137, 159–163
Forkmann, G., Heller, W., Grisebach, H. (1980) Anthocyanin biosynthesis in flowers of Matthiola incana. Flavanone 3-and flavonoid 3′-hydroxylases. Z. Naturforsch. Teil C. 35, 691–695
Grisebach, H. (1982) Biosynthesis of anthocyanins in: Anthocyanins as food colors, pp. 69–92, Markakis, P., ed. Academic Press, New York
Heller, W., Britsch, L., Forkamann, G., Grisebach, H. (1985) Leucoanthocyanidins as intermediates in anthocyanidin biosynthesis in flowers of Matthiola incana R. Br. Planta 163, 191–196
Kappert, H. (1957) Die Genetik des incana-Charakters und der Anthocyanbildung bei der Levkoje. Züchter 19, 140–145
Kristiansen, K.N. (1984) Biosynthesis of proanthocyanidins in barley: genetic control of the conversion of dihydroquercetin to catechin and proanthocyanidins. Carlsberg Res. Commun. 49, 503–524
Sandermann, H., Strominger, L. (1972) Purufication and properties of C55-isoprenoid alcohol phosphokinase from Staphylococcus aureus. J. Biol. Chem. 247, 5123–5131
Seyffert, W. (1982) Beiträge zur Genetik und Enzymologie der Flavonoide. Biol. Zentralbl. 101, 465–483
Seyffert, W. (1983) Homeostasis in defined genotypes of Matthiola incana, Theor. Appl. Genet. 64, 205–212
Spribille, R., Forkmann, G. (1981) Genetic control of chalcone synthase activity in flowers of Matthiola incana R. Br. Z. Naturforsch. Teil C. 36, 619–624
Stafford, H.A., Lester, H.H. (1982) Enzymic and nonenzymic reduction of (+)-dihydroquercetin to its 3,4-diol. Plant Physiol. 70, 695–698
Stafford, H.A., Lester, H.H. (1984) Flavan-3-ol biosynthesis. The conversion of (+)dihydroquercetin and flavan-3,4-cis-diol (leucocyanidin) to (+)catechin by reductase extracted from cell suspension cultures of Douglas fir. Plant Physiol. 76, 184–186
Stotz, G. (1983) Enzymologie und Genetik der Oxidationsreaktionen in der Flavonoid-Biosynthese höherer Pflanzen. Ph.D. Thesis, Universität Tübingen
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Heller, W., Forkmann, G., Britsch, L. et al. Enzymatic reduction of (+)-dihydroflavonols to flavan-3,4-cis-diols with flower extracts from Matthiola incana and its role in anthocyanin biosynthesis. Planta 165, 284–287 (1985). https://doi.org/10.1007/BF00395052
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DOI: https://doi.org/10.1007/BF00395052