Abstract
A biochemical model of C 3photosynthesis has been developed by G.D. Farquhar et al. (1980, Planta 149, 78–90) based on Michaelis-Menten kinetics of ribulose-1,5-bisphosphate (RuBP) carboxylase-oxygenase, with a potential RuBP limitation imposed via the Calvin cycle and rates of electron transport. The model presented here is slightly modified so that parameters may be estimated from whole-leaf gas-exchange measurements. Carbon-dioxide response curves of net photosynthesis obtained using soybean plants (Glycine max (L.) Merr.) at four partial pressures of oxygen and five leaf temperatures are presented, and a method for estimating the kinetic parameters of RuBP carboxylase-oxygenase, as manifested in vivo, is discussed. The kinetic parameters so obtained compare well with kinetic parameters obtained in vitro, and the model fits to the measured data give r 2values ranging from 0.87 to 0.98. In addition, equations developed by J.D. Tenhunen et al. (1976, Oecologia 26, 89–100, 101–109) to describe the light and temperature responses of measured CO2-saturated photosynthetic rates are applied to data collected on soybean. Combining these equations with those describing the kinetics of RuBP carboxylase-oxygenase allows one to model successfully the interactive effects of incident irradiance, leaf temperature, CO2 and O2 on whole-leaf photosynthesis. This analytical model may become a useful tool for plant ecologists interested in comparing photosynthetic responses of different C3 plants or of a single species grown in contrasting environments.
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Abbreviations
- PCO:
-
photorespiratory carbon oxidation
- PCR:
-
photosynthetic carbon reduction
- PPFD:
-
photosynthetic photon-flux density
- RuBP:
-
ribulose bisphosphate
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Harley, P.C., Weber, J.A. & Gates, D.M. Interactive effects of light, leaf temperature, CO2 and O2 on photosynthesis in soybean. Planta 165, 249–263 (1985). https://doi.org/10.1007/BF00395048
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DOI: https://doi.org/10.1007/BF00395048