Abstract
Glucose or non-metabolizable glucose analogues induce two systems of amino-acid transport in Chlorella vulgaris: an arginine-lysine system and a proline system. An additional third system of amino-acid transport is induced when glucose and an inorganic nitrogen source are present during glucose induction. The transport rates in glucose-NH +4 -treated cells are 10 to 80 times higher than in untreated cells. The transport system shows a rather broad specificity and catalyses the transport of at least ten neutral and acidic amino acids. Three of these amino acids (l-alanine, l-serine and glycine) are transported by the proline system as well. The system is specific for l-amino acids and has a pH optimum between 5 and 6. Transport by this system seems to be active, since amino acids are accumulated inside the cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cho, B.-H., Sauer, N., Komor, E., Tanner, W. (1981) Glucose induces two amino acid transport systems in Chlorella. Proc. Natl. Acad. Sci. USA 78, 3591–3594
Eddy, A.A. (1982) Mechanisms of solute transport in selected eucaryotic microorganisms. Adv. Microb. Physiol. 23, 1–78
Felle, H. (1981) Stereospecificity and electrogenicity of amino acid transport in Riccia fluitans. Planta 152, 505–512
Grenson, M., Hou, C., Crabeel, M. (1970) Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J. Bacteriol. 103, 770–777
Haaß, D., Tanner, W. (1974) Regulation of hexose transport in Chlorella vulgaris. Plant Physiol. 53, 14–20
Harrington, H.M., Henke, R.R. (1981) Amino acid transport into cultured tabacco cells. I. Lysine transport. Plant Physiol. 67, 373–378
Higgins, C.F., Ferro-Luzzi Ames, G. (1982) Regulatory regions of two transport operons under nitrogen control: nucleotide sequences. Proc. Natl. Acad. Sci. USA 79, 1083–1087
Komor, E., Thom, M., Maretzki, A. (1981) Mechanism of uptake of l-arginine by sugar-cane cells. Eur. J. Biochem. 116, 527–533
Oxender, D.L. (1972) Membrane transport. Annu. Rev. Biochem. 41, 777–814
Pedersen, Å.G., Knutsen, G. (1974) Uptake of l-Phenylalanine in synchronous Chlorella fusca. Characterization of the uptake system. Physiol. Plant. 32, 294–300
Richards, L., Thurston, C.F. (1980) Uptake of leucine and tyrosine and their intracellular pools in Chlorella fusca var. vacuolata. J. Gen. Microbiol. 121, 39–47
Sauer, N. (1982) Charakterisierung induzierbarer Aminosäureund Zuckertransportsysteme in Chlorella vulgaris. Ph.D. thesis, Universität Regensburg, FRG
Sauer, N., Komor, E., Tanner, W. (1983) Regulation and characterisation of two inducible amino-acid transport systems in Chlorella vulgaris. Planta 159, 404–410
Sauer, N., Tanner, W. (1984) Characterisation and partial purification of inducible transport proteins in Chlorella. Z. Pflanzenphysiol. 114, 367–375
Schwenke, J., Magana-Schwenke, N. (1969) Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim. Biophys. Acta 173, 302–312
Tanner, W., Loos, E., Kandler, O. (1966) Glucose assimilation of Chlorella in monochromatic light of 658 and 711 μm. In: Currents in photosynthesis, pp. 243–251, Thomas, J.B., Goedheer, J.D., eds. Donker Rotterdam
Tanner, W., Kandler, O. (1967) Die Abhängigkeit der Adaptation der Glucose-Aufnahme von der oxydativen und der photosynthetischen Phosphorylierung in Chlorella vulgaris. Z. Pflanzenphysiol. 58, 24–32
Van Sumere, D.F., Dedonder, A. (1971) The effect of some naturally occurring and synthetic phenolics and related compounds on the uptake and incorporation of phenylalanine-1-(14C) by Chlorella vulgaris. Z. Pflanzenphysiol. 65, 159–175
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sauer, N. A general amino-acid permease is inducible in Chlorella vulgaris . Planta 161, 425–431 (1984). https://doi.org/10.1007/BF00394573
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00394573