Summary
The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.
Article PDF
Avoid common mistakes on your manuscript.
References
Ballance DJ, Buxton FP, Turner G (1983) Biochem Biophys Res Commun 112:284–289
Ballance DJ, Turner G (1985) Gene 36:321–331
Case ME, Schweizer M, Kushner SR, Giles NH (1979) Proc Natl Acad Sci USA 76:5259–5263
Hynes MJ, Corrick CM, King JA (1983) Mol Cell Biol 3:1430–1439
Kelly JM, Hynes MJ (1985) EMBO J 4:475–479
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Penalva MA, Tourino A, Sanchez F, Patino C, Rubio V, Fernandez-Sousa JM, (1985) J Cell Biochem [Suppl 9c]:172
Rowlands RT, Turner G (1973) Mol Gen Genet 126:201–216
Tilburn J, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221
Turgeon BG, Garber RC, Yoder OC (1985) Mol Gen Genet 201:450–453
Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474
Author information
Authors and Affiliations
Additional information
Communicated by B. S. Cox
Rights and permissions
About this article
Cite this article
Beri, R.K., Turner, G. Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker. Curr Genet 11, 639–641 (1987). https://doi.org/10.1007/BF00393928
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00393928