Abstract
Hyphal fusion during initial stages of trap formation by Arthrobotrys oligospora was studied by video-enhanced contrast and electron microscopy. Trap initials grew perpendicularly to the parent hypha, then curved around and anastomosed with a peg that developed on the hypha. Trap initials usually developed 40–140 μm apart while the anastomosis occurred 20–25 μm from the initial. Vigorous cytoplasmic movements in trap initials and developed traps corresponded to intense staining with fluorescein diacetate (FDA) of these cells. In addition, bundles of microfilaments were seen in developing loops of traps. On fusion organelle migration took place from the tip cell of the trap into the peg. Later on a septum was formed at the site of fusion.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Barron G. (1981) Predators and parasites of microscopic animals. In: Cole G.T. & Kendrick B. (Eds) Biology of Conidial Fungi. Vol 2 (pp 167–200) — Academic Press, New York
Buller AHR (1931) Social organisation in Coprinus sterquilinus. In: Researches on Fungi. Vol 4 (pp 155–169). Longmans Green, London
Friman E, Olsson S & Nordbring-Hertz B (1985) Heavy trap formation by Arthrobotrys oligospora in liquid culture. FEMS Microbiol. Ecol. 31: 17–21
Gooday GW (1975) Chemotaxis and chemotropism in fungi and algae. In: Carlile MJ (Ed) Primitive Sensory and Communication Systems (pp 155–204) Academic Press, London
Gregory PH (1984) The first benefactors' lecture. The fungal mycelium: a historical perspective. Trans. Br. mycol. Soc. 82: 1–11
Higgins ML and Pramer D (1967) Fungal morphogenesis: Ring formation and closure by Arthrobotrys dactyloides. Science 155: 345–346
Jennings DH (1986) Morphological plasticity in fungi. In: Jennings DH & Trewavas AJ (Eds) Plasticity in Plants. Symp. Soc. Exp. Biol. 40: 329–346
Kreger-van Rij NJW and Veenhuis M (1969) A study of vegetative reproduction in Endomycopsis platypodis by electron microscopy. J. Gen. Microbiol. 58: 341–346
Markham P and Collinge AJ (1987) Woronin bodies of filamentous fungi. FEMS Microbiol. Rev. 46: 1–11
Nordbring-Hertz B (1973) Peptide-induced morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. Physiol. Plant. 29: 223–233
Nordbring-Hertz B (1977) Nematode-induced morphogenesis in the predacious fungus Arthrobotrys oligospora. Nematologica 23: 443–451
Nordbring-Hertz B, Veenhuis M and Harder W (1984) Dialysis membrane technique for ultrastructural studies of microbial interactions. Appl. Environ. Microbiol. 47: 195–197
Nordbring-Hertz B, Zunke U, Wyss U and Veenhuis M (1986) Trap formation and capture of nematodes by Arthrobotrys oligospora. Film C1622. Inst. Wiss. Film, Göttingen, Germany
Söderström BE (1977) Vital staining of fungi in pure cultures and in soil with fluorescein diacetate. Soil Biol. Biochem. 9: 59–63
Veenhuis M, Nordbring-Hertz B & Harder W (1984) Occurrence, characterization and development of two different types of microbodies in the nematophagous fungus Arthrobotrys oligospora. FEMS Microbiol. Lett. 24: 31–38
Veenhuis M, Nordbring-Hertz B and Harder W (1985a) An electron-microscopical analysis of capture and initial stages of penetration of nematodes by Arthrobotrys oligospora. Antonie van Leewenhoek 51: 385–398
Veenhuis M, Nordbring-Hertz B and Harder W (1985b) Development and fate of electron-dense microbodies in trap cells of the nematophagous fungus Arthrobotrys oligospora. Antonie van Leeuwenhoek 51: 399–407
Veenhuis M, Nordbring-Hertz B and Harder W (1985c) An ultrastructural study of cell-cell interactions in capture organs of the nematophagous fungus Arthrobotrys oligospora FEMS Microbiol. Lett. 30: 93–98
Wyss U and Zunke U (1986) The potential of high resolution video-enhanced contrast microscopy in nematological research. Rev. Nématol. 9: 91–94
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nordbring-Hertz, B., Friman, E. & Veenhuis, M. Hyphal fusion during initial stages of trap formation in Arthrobotrys oligospora . Antonie van Leeuwenhoek 55, 237–244 (1989). https://doi.org/10.1007/BF00393852
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393852