Abstract
The uptake of D-mannose was studied in detached cotyledons of germinated fenugreek (Trigonella foenum-graecum L.) seeds. Uptake kinetics indicate the involvement of two components, a saturable component operating at low concentrations and a diffusion-like one at high concentrations. Treatment of cotyledons with carbonyl-cyanide-m-chlorophenylhydrazone and p-chloromercuribenzenesulfonic acid reduced D-mannose-uptake rates by about 35% and 35–65%, respectively. No difference in the uptake rates was observed in the presence of D-galactose or 3-O-methylglucose. D-Mannose uptake was not very much affected by pH. The optimum pH for its uptake was 6.5 while at pH 8.5 its uptake was reduced by 22%. D-Mannose addition to fenugreek cotyledons did not induce alkalinization of the medium. Furthermore, low turgor, which enhances proton/sugar cotransport, decreased D-mannose uptake while the uptake of 3-O-methylglucose was increased. The rate of D-mannose uptake by fenugreek cotyledons depended on the hours of imbibition. These changes of uptake were not followed by analogous changes in the turgor pressure (Ψp) of fenugreek cotyledons, which remained fairly constant. Results indicate that D-mannose is partially taken up by a carrier which has high specificity for D-mannose, but not by a H+-sugar cotransport system. It is further concluded that the carrier plays an important role in switching on and off the uptake capacity of fenugreek cotyledons during seedling development.
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Abbreviations
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- DTT:
-
dithiothreitol
- 3-OMG:
-
3-O-methylglucose
- PCMBS:
-
p-chloromercuribenzensulfonic acid
- ψ:
-
water potential
- Ψs :
-
osmotic potential
- Ψp :
-
turgor pressure
References
Cameron-Mills, V., Duffus, C.M. (1979) Hexose transport in isolated immature barley embryos. Ann. Bot. 44, 485–494
Colombo, R., De Michaelis, M.T., Lade, P. (1978) 3-O-Methylglucose uptake stimulation by auxin and by fusicoccin in plant materials and its relationships with proton extrusion. Planta 138, 249–256
Daie, J., Wilusz, J.E. (1987) Facilitated transport of glucose in isolated phloem segments of celery. Plant Physiol. 85, 711–715
Delrot, S., Despeghel, J.P., Bonnemain, J.L. (1980) Phloem loading in Vicia faba leaves: effect of N-ethylmaleimidine and para-chloromercuribenzene sulfonic acid on H+ extrusion, K+ and sucrose uptake. Planta 149, 144–148
Giaquinta, R. (1976) Evidence for phloem loading from the apoplast. Chemical modification of membrane sulfhydryl groups. Plant Physiol. 57, 872–875
Giaquinta, R. (1983) Phloem loading of sucrose. Ann. Rev. Plant Physiol. 34, 347–387
Griffith, S.M., Jones, R.J., Brenner, M.L. (1987) In vitro sugar transport in Zea mays L. II. Characteristics of sugar absorption and metabolism by isolated developing embryos. Plant Physiol. 84, 472–475
Kinraide, T.B., Wyse, R.E. (1986) Electrical evidence for turgor inhibition of proton extrusion in sugar beet taproot. Plant Physiol. 82, 1148–1150
Komor, E., (1977) Sucrose uptake by cotyledons of Ricinus communis L.: characteristics, mechanism and regulation. Planta 137, 119–131
Komor, E. (1982) Transport of sugars. In: Encyclopedia of plant physiology, N.S., vol. 13A: Plant carbohydrates I, pp. 635–676, Loewus, F.A., Tanner, W., eds. Springer, Berlin Heidelberg New York
Lichtner, F.T., Spanswick, R.M. (1981) Sucrose uptake by developing soybean cotyledons. Plant Physiol. 68, 693–698
M'Batchi, B., Pichelin, D., Delrot, S. (1987) Selective solubilisation of membrane protein differentially labeled by p-chloromercuribenzenesulfonic acid in the presence of sucrose. Plant Physiol. 83, 541–545
McCleary, B.V., Matheson, N.K. (1976) Galactomannan utilization in germinating legume seeds. Phytochemistry 15, 43–47
Monoury, G., Delrot, S., Bonnemain, J.L. (1984) Energetics of threonine uptake by pod wall tissues of Vicia faba L. Planta 161, 178–185
Reid, J.S.G. (1971) Reserve carbohydrate metabolism in germinating seeds of Trigonella foenum-graecum L. (Leguminosae). Planta 100, 131–142
Reid, J.S.G., Davies, C., Meier, H. (1977) Endo-β-mannanase, the leguminous aleurone layer and the storage galactomannanan in germinating seeds of Trigonella foenum-graecum L. Planta 133, 219–222
Reid, J.S.G., Meier, H. (1973) Enzymatic activities and galactomannan mobilisation in germinating seeds of fenugreek (Trigonella foenum-graecum L. Leguminosae). Secretion of α-galactosidase and β-mannosidase by the aleurone layer. Planta 112, 301–308
Reinhold, L., Kaplan, A., (1984) Membrane transport of sugars and amino acids. Annu. Rev. Plant Physiol. 35, 45–83
Reinhold, L., Seiden, A., Volokita, M. (1984) Is modulation of the rate of proton pumping a key event in osmoregulation? Plant Physiol. 75, 941–946
Seiler, A. (1977) Galactomannanabbau in keimenden Johannisbrotsamen (Ceratonia siliqua L.) Planta 134, 209–221
Spyropoulos, C.G. (1986) Osmoregulation, growth and sucrose accumulation in germinated Trigonella foenum-graecum L. seeds treated with polyethylene glycol. Physiol. Plant. 68, 129–135
Spyropoulos, C.G., Lambiris, M.P. (1980) Effect of water stress on germination and reserve carbohydrate metabolism in germinating seeds of Ceratonia siliqua L. J. Exp. Bot. 31, 851–857
Spyropoulos, C.G., Reid, J.S.G. (1985) Regulation of α-galactosidase activity and the hydrolysis of galactomannan in the endosperm of the fenugreek (Trigonella foenum-graecum L.) seed. Planta 166, 271–275
Spyropoulos, C.G., Reid, J.S.G. (1988) Water stress and galactomannan breakdown in germinating fenugreek seeds. Stress affects the production and the activities in vivo of galactomannan-hydrolysing enzymes. Planta 174, 473–478
Tomos, D.A., Leigh, R.A., Shaw, C.A., Wyn Jones, G. (1984) A comparison of methods for measuring turgor pressures and osmotic pressures of cells of red beet storage tissue. J. Exp. Bot. 35, 1675–1683
Uebelmann, G. (1978) Samenkeimung bei Trigonella foenum-graecum L. Aufnahme des beim Galactomannanabbau im Endosperm freiwerdenden Zuckers durch den Embryo. Z. Pflanzenphysiol. 88, 235–253
Wyse, R.E., Zamski, E., Deri Tomos, A. (1986) Turgor regulation of sucrose transport in sugar beet taproot system. Plant Physiol. 81, 478–481
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Zambou, K., Spyropoulos, C.G. D-Mannose uptake by fenugreek cotyledons. Planta 179, 403–408 (1989). https://doi.org/10.1007/BF00391087
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DOI: https://doi.org/10.1007/BF00391087