Abstract
Protoplasts have been obtained from vegetative thallus of the green seaweed Enteromorpha following enzymic digestion with driselase and pectinase. The viability of purified protoplast fractions was assessed by staining and measurements of O2 uptake and evolution.
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Abbreviations
- MES:
-
2-(N-morpholino) ethanesulphonic acid
- TES:
-
N-tris(hydoxymethyl) methyl-2 aminoethanesulphonic acid
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Millner, P.A., Callow, M.E. & Evans, L.V. Preparation of protoplasts from the green alga Enteromorpha intestinalis (L.) Link. Planta 147, 174–177 (1979). https://doi.org/10.1007/BF00389521
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DOI: https://doi.org/10.1007/BF00389521