Summary
An investigation of the fine structure of the cell wall was carried out on representative species of four morphological forms of collenchyma, viz. annular, angular, plate and lacunate. In all forms lamellae were observed in which the orientation of cellulose microfibrills was transverse. These lamellae alternated throughout the thickness of the wall with lamellae in which the orientation of cellulose microfibrils was longitudinal. The distribution of pectic substances within the wall, when stained with ruthenium red and by the alkaline hydroxylamine-ferric chloride method of Reeve (1958), was generally not observed in lamellate form although instances of lamellae with high pectic content were observed.
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Albersheim, P., Mühlethaler, K., Frey-Wyssling, A.: Stained pectin as seen in the electron microscope. J. biophys. biochem. Cytol. 8, 501–506 (1960).
Anderson, D.: Über die Struktur der Kollenchymzellwand auf Grund mikrochemischer Untersuchungen. Abh. Akad. Wiss. Wien, Math.-Nat. Kl. 13b, 429–440 (1927).
Beer, M., Setterfield, G.: Fine structure in thickened primary walls of collenchyma cells in celery petioles. Amer. J. Bot. 45, 571–580 (1958).
Czaja, A. T.: Neue Untersuchungen über die Struktur der partiellen Wandverdickungen von faserförmigen Kollenchymzellen. Planta (Berl.) 56, 109–124 (1961).
Jensen, W. A.: Botanical histochemistry. San Francisco-London: Freeman 1962.
Majumdar, G. P., Preston, R. D.: The fine structure of collenchyma cells in Heracleum sphondylium L. Proc. roy Soc. B 130, 201–217 (1941).
Mayor, H. D., Hampton, J. C., Rosario, B.: A simple method for removing the resin from epoxy-embedded tissue. J. Cell Biol. 9, 909–910 (1961).
Preston, R. D., Duckworth, R. B.: The fine structure of the walls of collenchyma cells of Petasites vulgaris L. Proc. Leeds phil. Soc. 4, 343–351 (1946).
Reeve, R. M.: A specific hydroxylamine-ferric chloride reaction for histochemical localization of pectin. Stain Technol. 34, 209–211 (1958).
Roelofson, P. A.: Ultrastructure of the wall of growing cells and its relation to the direction of growth. Adv. Bot. Res. 2, 69–149 (1966).
Roland, J. C.: Infrastructure des membranes du collenchyme. C. R. Acad. Sci. (Paris) 259, 4331–4334 (1964).
—: Edification et infrastructure de la membrane collenchymateuse. Son remaniement lors de la sclerification. C. R. Acad. Sci. (Paris) 260, 950–953 (1965).
—: Organization de la membrane paraplasmique du collenchyme. J. Microscopie 5, 323–348 (1966).
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The author would like to express appreciation and gratitude to Prof. A. B. Wardrop under whose direction this research was conducted and who provided numerous helpful ideas and criticisms both during the experimental work and in the compilation of the manuscript. Thanks are conveyed as well to Mr. F. J. Daniels and Mr. G. M. Harwood for technical assistance, and to Drs. D. A. Griffiths and I. A. Staff for reading the manuscript.
The project was carried out with the financial assistance of the Forest Products Research Laboratory, Ottawa, Canada. The author, who is on educational leave from this institution, wishes to express his appreciation for its support. For key to figures, see p. 14.
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Chafe, S.C. The fine structure of the collenchyma cell wall. Planta 90, 12–21 (1969). https://doi.org/10.1007/BF00389292
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DOI: https://doi.org/10.1007/BF00389292