Abstract
The inhibitory effect of propionic acid P and biomass concentration X is studied in batch and continuous fermentations with cell recycle.
In batch fermentations, the specific growth rate decreases and cancels out at a critical propionic acid concentration Pc 1; the formerly decreasing specific production rate becomes constant after Pc 1 and cancels out when a second critical propionic acid concentration Pc 2 is reached.
In continuous fermentation with cell recycle, a similar inhibition is observed with biomass. The specific rates decrease and become constant at a critical biomass concentration Xc. They cancel out at different high biomass concentrations.
In both cases, the specific production rate can be related to the specific growth rate by the Luedeking and Piret expression: ν=αμ+β, [1], where the constants α and β are determined by the fermentation parameters.
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Abbreviations
- t h:
-
time
- X kg/m3 :
-
biomass concentration
- P kg/m3 :
-
propionic acid concentration
- A kg/m3 :
-
acetic acid concentration
- S kg/m3 :
-
lactose concentration
- dX/dt kg/(m3h):
-
instantaneous rate of cell growth
- dP/dt kg/(m3h):
-
instantaneous rate of propionic acid production
- μ h−1 :
-
specific growth rate
- ν h−1 :
-
specific propionic acid production rate
- D h−1 :
-
dilution rate
References
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Blanc, P., Goma, G. Kinetics of inhibition in propionic acid fermentation. Bioprocess Engineering 2, 175–179 (1987). https://doi.org/10.1007/BF00387325
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DOI: https://doi.org/10.1007/BF00387325