Summary
Sex determination in the monogenic blowfly Chrysomya rufifacies is controlled by a dominant or epistatic female sex realizer (F′) having sex predetermining properties (F′/f=female-producing female; f/f=male-producing female or male, respectively). To determine (1) the cell type in which the maternal effect gene F′ is expressed. and (2) the autonomous or nonautonomous sexual differentiation of the germ cells germ-line mosaics were constructed in C. rufifacies by pole-cell transplantations. The production of bisexual progeny by germ-line mosaics generated by transplanting pole cells between both types of female embryos shows that the F′ gene product is synthesized by germ-line cells themselves, not by maternal (intra- or extraovarian) somatic cells. Pole cell transplantations between male and female embryos yielded completely fertile heterosexual germ-line mosaics thus demonstrating phenotypic sex reversal of donor germ cells in a host of the opposite sex. Consequently, the sexual differentiation of a germ cell in C. rufifacies is not determined by its own genotypic constitution but is induced by the surrounding somatic cells.
The male sex of F′/f individuals generated by fertilization with F′-bearing sperm from a heterosexual germ-line mosaic indicates that the F′ gene is either not expressed during spermatogenesis and early embryogenesis or is expressed too late or in not sufficient amounts to direct differentiation into the female sex. This finding is consistent with the assumption that progamic expression of F′ is found exclusively during oogenesis in F′/f females.
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References
Anderson DT (1972) The development of holometabolous insects. In: Counce SJ, Waddington CH (eds) Developmental systems: Insects, vol 1. Academic Press, London, New York, pp 165–242
Bacci G (1965) Sex determination. Pergamon Press Oxford, London, Edinburgh, New York, Toronto, Paris, Frankfurt
Becak W (1983) Evolution and differentiation of sex chromosomes in lower vertebrates. Differentiation 23 (Suppl):3–12
Briggs R, Justus JT (1968) Partial characterization of the component from normal eggs which corrects the maternal effect of gene o in the Mexican axolotl (Ambystoma mexicanum). J Exp Zool 167:105–115
Chan STH, O WS (1981) Environmental and non-genetic mechanisms in sex determination. In: Austin CR, Edwards RG (eds) Mechanisms of sex differentiation in animals and man. Academic Press, London, New York, Toronto, Sydney, San Francisco, pp 55–111
Crouse HV (1960) The nature of the influence of X-translocations on sex of progeny in Sciara coprophila. Chromosoma (Berl) 11:146–166
Crouse HV (1965) Experimental alterations in the chromosome constitution of Sciara. Chromosoma (Berl) 16:391–410
Dävring L, Sunner M (1982) A lampbrush phase in oocytes of Drosophila and its bearing upon mutagen sensitivity data. Hereditas 97:247–259
Davidson EH (1976) Gene activity in early development, 2nd edn. Academic Press, New York, San Francisco, London
Davis CWC (1967) A comparative study of larval embryogenesis in the mosquito Culex fatigans Wiedemann (Diptera: Culicidae) and the sheepfly Lucilia sericata Meigen (Diptera: Calliphoridae). I. Description of embryonic development. Aust J Zool 15:547–579
Deusen EB van (1976) Sex determination in germ line chimeras of Drosophila melanogaster. J Embryol Exp Morph 37:173–185
Engstrom L, Caulton JH, Underwood EM, Mahowald AP (1982) Developmental lesions in the agametic mutant of Drosophila melanogaster. Dev Biol 91:163–170
Fischer A (1977) Autonomy for a specific gene product in oocytes: Experimental evidence in the poylchaetous annelid, Platynereis dumerilii. Dev Biol 55:46–58
Illmensee K (1973) The potentialities of transplanted early gastrula nuclei of Drosophila melanogaster. Production of their imago descendants by germ-line transplantation. Wilhelm Roux' Arch 171:331–343
Illmensee K (1978) Drosophila chimeras and the problem of determination. In: Gehring WJ (ed) Results and problems in cell differentiation, vol 9, genetic mosaics and cell differentiation. Springer, Berlin, heidelberg, New York, pp 51–69
Jung E (1966) Untersuchungen am Ei des Speisebohnenkäfers Bruchidius obtectus Say (Coleoptera). I. Mitteilung. Entwicklungs-geschichtliche Ergebnisse zur Kennzeichnung des Eitypus. Z Morphol Ökol Tiere 56:444–480
Kühn A, Caspari E, Plagge E (1935) Über hormonale Genwirkungen bei Ephestia kühniella. Nachr Ges Wiss Göttingen, Math Phys K1. Fachgruppe 2:1–29
McCarrey JR, Abbott UK (1979) Mechanisms of genetic sex determination, gonadal sex differentiation, and germ-cell development in animals. Adv Genet 20:217–290
Marsh JL, Deusen EB van, Wieschaus E, Gehring WJ (1977) Germ line dependence of the deep orange maternal effect in Drosophila. Dev Biol 56:195–199
Marsh JL, Wieschaus E (1977) Germ-line dependence of the maroon-like maternal effect in Drosophila. Dev Biol 60:396–403
Marsh JL, Wieschaus E (1978) Is sex determination in germ line and soma controlled by separate genetic mechanisms? Nature 272:249–251
Metz CW (1938) Chromosome behavior, inheritance and sex determination in Sciara. Am Nat 72:485–520
Metz CW, Schmuck ML (1929) Further studies on the chromosome mechanism responsible for unisexual progenies in Sciara. Tests of “exceptional” males. Proc Natl Acad Sci USA 15:867–870
Mittwoch U (1973) Genetics of sex differentiation. Academic Press, New York, San Francisco, London
Regenass U, Bernhard HP (1978) Analysis of the Drosophila maternal effect mutant mar(3)1 by pole cell transplantation experiments. Mol Gen Genet 164:85–91
Roy DN, Siddons LB (1939) On the life history and bionomics of Chrysomyia rufifacies Macq. (Order Diptera, Family Calliphoridae). Parasitology 31:442–447
Scheel A-C (1981) Polzellbildung bei der monogenen Fliege Chrysomya rufifacies (Diptera, Calliphoridae). Staatsexamensarbeit, Zoologisches Institut, Universität Kiel
Schmid M (1983) Evolution of sex chromosomes and heterogametic systems in Amphibia. Differentiation 23 (Suppl):13–22
Turner FR, Mahowald AP (1976) Scanning electron microscopy of Drosophila embryogenesis. 1. The structure of the egg envelopes and the formation of the cellular blastoderm. Dev Biol 50:95–108
Ullerich F-H (1963) Geschlechtschromosomen und Geschlechts-bestimmung bei einigen calliphoriden (Calliphoridae, Diptera). Chromosoma (Berl) 14:45–110
Ullerich F-H (1973) Die genetische Grundlage der Monogenie bei der Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera). Mol Gen Genet 125:157–172
Ullerich F-H (1975) Identifizierung der genetischen Geschlechtschromosomen bei der monogenen Schmeißfliege Chrysomya rufifacies (Calliphoridae, Diptera). Chromosoma (Berl) 50:393–419
Ullerich F-H (1976) Chromosomenverhältnisse, konstitutives Heterochromatin und Geschlechtsbestimmung bei einigen Arten der Gattung Chrysomya (Calliphoridae, Diptera). Chromosoma (Berl) 58:113–136
Ullerich F-H (1980) Analysis of the predetermining effect of a sex realizer by ovary transplantations in the monogenic fly Chrysomya rufifacies. Wilhelm Roux' Arch 188:37–43
Ullerich F-H (1981) Autonomie der Ovar-Anlagen für das Genprodukt eines prädeterminierenden Geschlechtsrealisators bei der monogenen Fliege Chrysomya rufifacies (Diptera: Calliphoridae). Entom Gen 7:113–119
Ullerich F-H (1982) Germ line autonomy for the maternal effect of a sex realizer in the monogenic blowfly Chrysomya rufifacies. Naturwissenschaften 69:342–343
Ullerich F-H (1983) Sex reversal in transplanted primordial germ cells of the monogenic blowfly Chrysomya rufifacies. Naturwissenschaften 70:256–257
Wieschaus E, Marsh JL, Gehring W (1978) fs (1) K10, a germline-dependent female sterile mutation causing abnormal chorion morphology in Drosophila melanogaster. Wilhelm Roux' Arch 184:75–82
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Ullerich, FH. Analysis of sex determination in the monogenic blowfly Chrysomya rufifacies by pole cell transplantation. Molec. Gen. Genet. 193, 479–487 (1984). https://doi.org/10.1007/BF00382087
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DOI: https://doi.org/10.1007/BF00382087