Abstract
Callus cultures of two low-alkaloid lines of Nicotiana tabacum L. had considerably lower nicotine contents than cultures from the respective highalkaloid cultivars which were isogenic except for the two loci for alkaloid accumulation. Thus, there was a strong correlation between the nicotine content of callus cultures and the plants from which they were derived.
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References
Chaplin, J.F. (1975) Registration of LAFC 53 tobacco germplasm. Crop Sci. 15, 282
Legg, P.D., Collins, G.B., Litton, C.C. (1970) Registration of LA Burley 21 tobacco germplasm. Crop Sci 10, 212
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497
Ohta, S., Yatayawa, M. (1978) Effect of light on nicotine production in tobacco tissue culture. Agric. Biol. Chem. 42, 873–877
Ohta, S., Matsui, O., Yatayawa, M. (1978) Culture conditions for nicotine production in tobacco tissue culture. Agric. Biol. Chem. 42, 1245–1251
Roller, V. (1978) Selection of plants and plant tissue cultures of Catharanthus roseus with high content of serpentine and ajmalicine, Proc. Int. Symp. on plant cell culture, BPT-Rep. 1/78. In: Production of natural compounds by cell culture methods, pp. 95–104, Alfermann, A.W., Reinhard, E., eds. München (Munich): Ges. für Strahlen- u. Umweltforsch., Bereich Projektträgerschaften
Tabata, M., Hiraoka, N. (1976) Variation of alkaloid production in Nicotiana rustica callus cultures. Physiol. Plant. 38, 19–23
Willits, C.O., Swain, M.L., Connelly, J.A., Brice, B.A. (1950) Spectrophotometric determination of nicotine. Anal. Chem. 22, 430–433
Zenk, M.H., El-Shagi, H., Arens, T., Stockigt, J., Weiler, E.W., Deus, B. (1976) Formation of indole alkaloids serpentine and ajmalicine in cell suspension cultures of Catharanthus roseus. In: Plant tissue culture and its bio-technological application, pp. 3–16, Barz, W., Reinhard, E., Zenk, M.H., eds. Springer, Berlin Heidelberg New York
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Kinnersley, A.M., Dougall, D.K. Correlation between the nicotine content of tobacco plants and callus cultures. Planta 149, 205–206 (1980). https://doi.org/10.1007/BF00380884
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DOI: https://doi.org/10.1007/BF00380884