Abstract
We analysed human papillomavirus (HPV) infections in 61 tissue specimens of skin warts of Taiwanese patients by DNA hybridization. The prevalence of HPV infection was 69% by Southern blot hybridization. The typing of HPVs was performed by dot blot hybridization under highly stringent conditions with each probe separately. The prevalence of HPV-1, 2/3, 4, 5, 8, 11, 16 and 18 in skin warts was 13, 7, 16, 2, 0, 5, 2 and 8%, respectively. Chi-squared analysis revealed that there was a correlation between HPV type and copy number. Most HPV-4-induced warts were verruca vulgaris. HPV-1 DNA was detected in verruca plantaris and verruca vulgaris. No specific histopathological features were found to be indicative of the presence or absence of HPV, or of the various types of HPV infection.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Anisimova E, Bartak P, Vlcek D, Hirsch I, Brichacek B, Vonka V (1990) Presence and type specificity of papillomavirus antibodies demonstrable by immunoelectron microscopy tests in samples from patients with warts. J Gen Virol 71: 419–422
Beaudenon S, Praerouius F, Kremsdorf D, Lutzner M, Worsaae N, Pehau-Arnaudet G, Orth G (1987) A new type of human papillomavirus associated with oral focal epithelial hyperplasia. J Invest Dermatol 88: 130–135
Chen SL, Mounts P (1990) Transformation activity of the E5a protein of human papillomavirus type 6 in NIH3T3 and C127 cells. J Virol 64: 3226–3233
Corley E, Pueyo S, Goc B, Diaz A, Zorzopulos J (1988) Papillomaviruses in human skin warts and their incidence in an Agentine papulation. Diagn Microbiol Infect Dis 10: 93–101
Crum CP, Ikenberg H, Richart RM (1984) Human papillomavirus type 16 and early cervical neoplasia. N Engl J Med 310: 880–883
Denhardt D (1966) A membrane filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23: 641–646
Durst M, Gissmann L, Ikenberg H, Hausen H zur (1983) A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proc natl. Acad Sci USA 80: 3812–3815
Fleming KA, Venning V, Evans M (1987) DNA typing of genital warts and diagnosis of sexual abuse of children. Lancet 2: 454
Grimmel M, de Villiers EM, Neumann C, Pawlita M, Hausen H zur (1988) Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas. Int J Cancer 41: 5–9
Gross G, Pfister H, Hagedorn M, Gissmann L (1982) Correlation between human papillomavirus (HPV) type and histology of warts. J Invest Dermatol 78: 160–164
Grussendorf-Conen EI, Gissmann L, Holters J (1983) Correlation between content of viral DNA and evidence of mature virus particles in HPV-1, HPV-4, and HPV-6 induced virus acanthomata. J Invest Dermatol 81: 511–513
Hallam N, Gibson P, Green J, Charnock M (1989) Detection and typing of human papillomavirus infection of the uterine cervix by dot blot hybridization: comparison of scrapes and biopsies. J Med Virol 27: 317–321
Hausen H zur (1977) Human papilloma viruses and their possible role in squamous cell carcinomas. Curr Top Microbiol Immunol 78: 1–30
Hausen H zur, Gissmann L, Schlehofer R (1984) Viruses in the etiology of human genital cancer. Prog Med Virol 30: 170–186
Heilman CA, Law MF, Israel MA, Howley PM (1980) Cloning of human papillomavirus genomic DNAs and analysis of homologous polynucleotide sequences. J Virol 36: 395–407
Jenson AB, Lim LY, Singer AE (1989) Comparison of human papillomavirus type 1 serotyping by monoclonal antibodies with genotyping by in situ hybridization of plantar warts. J Cutan Pathol 16: 54–59
Lever WR (1990) Diseases caused by viruses, Histopathology of the skin, vol 21, J. B. Lippincott Company, pp 399–425
Mounts P, Shah KV, Kashima H (1982) Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx. Proc Natl Acad Sci USA 79: 5425–5429
Obalek S, Jablonska S, Favre M, Walczak L, Orth G (1990) Condylomata acuminata in children: frequent association with human papillomaviruses responsible for cutaneous warts. J Am Acad Dermatol 23: 205–213
Orth G, Jablonska S, Favrf M, Croissant O, Obalek S, Jarzabek-Chorzelska M (1981) Identification of papillomaviruses in butchers' warts. J Invest Dermatol 76: 97–102
Pfister H, Hettich I, Runne U, Gissmann L, Chilf GN (1983) Characterization of human papillomavirus type 13 from focal epithelial hyperplasia neck lesions. J Virol 47: 363–366
Schneider A, Kirchmayr R, Villiers EM de, Gissmann L (1988) Subclinical human papillomavirus infections in male sexual partners of female carriers. J Urol 140: 1431–1434
Sytwu HK, Sheu WJ, Shih JM, Chen SL (1991) Detection and analysis of human papillomavirus type 1 infection of skin warts from a dermatology clinic in Taiwan by Southern hybridization Chin. J Microbiol Immunol 24: 201–202
Ullman EV (1923) On the etiology of the laryngeal papilloma. Acta Otolaryngold (Stockh) 5: 317–325
Villiers EM de (1989) heterogeneity of the human papillomavirus group. J Virol 63: 4898–4903
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, S.L., Tsao, Y.P., Lee, J.W. et al. Characterization and analysis of human papillomaviruses of skin warts. Arch Dermatol Res 285, 460–465 (1993). https://doi.org/10.1007/BF00376818
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00376818