Abstract
To further evaluate the mechanisms of oxalate (Ox2−) transport in the intestine the following studies were performed using isolated, short-circuited segments of the rabbit distal colon (DC). In control buffer, the DC absorbed Ox2− (net Ox2− flux, J OxNet =5.4±0.7 pmol · cm−2 · h−1). Replacement of Na+ with N-methyl-d-glucamine (NMDG+) abolished Ox2− absorption by decreasing mucosal to serosal Ox2− flux (J Oxms ), without affecting Cl− transport, while gluconate substitution for Cl− did not affect J OxNet or net Na+ flux (J NaNet ). Addition of Na+ to the serosal side of tissues bathed by NMDG+ buffer increased J Oxms 40% without altering mucosal to serosal Cl− flux (J Clms ). Serosal amiloride or dimethyl amiloride (10−3 M) abolished J OxNet by decreasing J Oxms , it increased serosal to muscosal Cl− flux (J Clsm ) and it gradually inhibited short-circuit current (I sc). Mucosal amiloride (10−4 M) abolished I sc but had no effect on Ox2− or Cl− fluxes. Serosal 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS, 10−6 M) reduced J Oxms by 20% and J OxNet by 43% without affecting J Clms or J ClNet . Dibutyryl cyclic adenosine monophosphate (dB-cAMP, 5×10−4 M, both sides) stimulated Ox2− secretion (J OxNet = −12.6±3.3 pmol · cm−2 · h−1). The dB-cAMP-induced secretion of Ox2− and Cl− were fully abolished by serosal furosemide (10−4 M) and partially inhibited (35%) by 5×10−4 M mucosal NPPB [5-nitro-2-(3-phenylpropylamino)-benzoic acid], a putative Cl− channel blocker. It is proposed that: (1) basal absorption of Ox2−, but not Cl−, is dependent upon a previously undescribed basolateral Na+-H+ exchanger that may be coupled to a DIDS-sensitive, basolateral anion exchange system that mediates Ox2− flux; (2) the DC secretes Ox2− in response to dB-cAMP by a mechanism that is indistinguishable from the pathway for Cl− secretion.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Benos DJ (1982) Amiloride: a molecular probe of sodium transport in tissues and cells. Am J Physiol 242:C131-C145
Binder HJ (1974) Intestinal oxalate absorption. Gastroenterology 67:441–446
Bridges RJ, Worrell RT, Frizzel RA, Benos DJ (1989) Stilbene disulfonate blockade of colonic secretory Cl− channels in planar lipid bilayers. Am J Physiol 256:C902-C912
Diener M, Rummel W (1989) Actions of the Cl− channel blocker NPPB on absorptive and secretory transport processes of Na+ and Cl− in rat descending colon. Acta Physiol Scand 137:215–222
Dobbins JW, Binder HJ (1977) Importance of the colon in enteric hyperoxaluria. N Engl J Med 296:298–301
Dreinhofer J, Gogelein H, Greger R (1988) Blocking kinetics of Cl− channels in colonic carcinoma cells (HT29) as revealed by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NNPB). Biochim Biophys Acta 946:135–142
Dudeja PK, Foster ES, Brasitus TA (1989) Na+-H+ antiporter of rat colonic basolateral membrane vesicles. Am J Physiol 257:G624-G632
Earnest DL (1974) Enteric hyperoxaluria. Adv Intern Med 24:407–427
Finlayson B (1977) In: David DS (ed) Calcium metabolism in renal failure and nephrolithiasis. Wiley, New York, pp 354–357
Freel RW (1987) Dihydroxy bile salt-induced secretion of rubidium ion across the rabbit distal colon. Am J Physiol 252:G554-G561
Freel RW, Hatch M, Earnest DL, Goldner AM (1980) Oxalate transport across the isolated rat colon. A re-examination. Biochim Biophys Acta 600:838–843
Frizzel RA, Koch MJ, Schultz SG (1976) Ion transport by rabbit colon. I. Active and passive components. J Membr Biol 27:297–316
Halm DR, Frizzell RA (1986) Active K transport across rabbit distal colon: relation to Na absorption and Cl secretion. Am J Physiol 251:C252-C267
Hatch M, Vaziri ND (1991) Segmental differences in intestinal oxalate transport. FASEB J 5:A1138
Hatch M, Freel RW, Goldner AM, Earnest DL (1984) Oxalate and chloride absorption by the rabbit colon: sensitivity to metabolic and anion transport inhibitors. Gut 25:232–237
Heintze K, Stewart CP, Frizzell RA (1983) Sodium-dependent chloride secretion across rabbit descending colon. Am J Physiol 244:G357-G365
Keeling DJ, Taylor AG, Smith PL (1991) Effects of NPPB [5-nitro-2-(3-phenylpropylamino)benzoic acid] on chloride transport in intestinal tissues and the T84 cell line. Biochim Biophys Acta 1115:42–48
Kleyman R, Cragoe EJ (1990) Cation transport probes: the amiloride series. Methods Enzymol 191:739–755
Knickelbein RG, Dobbins JW (1990) Sulfate and oxalate exchange for bicarbonate across the basolateral membrane of rabbit ileum. Am J Physiol 259:G807-G813
Knickelbein RG, Aronson PS, Dobbins JW (1986) Oxalate transport by anion exchange across rabbit ileal brush border. J Clin Invest 77:170–175
McCabe RD, Smith PL (1985) Colonic potassium and chloride secretion: role of cAMP and calcium. Am J Physiol 248:G103-G109
O'Grady SM, Palfrey HC, Field M (1987) Characteristics and functions of Na-K-Cl cotransport in epithelial tissues. Am J Physiol 253:C177-C192
Rajendran V, Binder HJ (1993) Cl-HCO3 and Cl-OH exchanges mediate Cl uptake in apical membrane vesicles of rat distal colon. Am J Physiol 264:G874-G879
Rajendran VM, Oesterlin M, Binder HJ (1991) Sodium uptake across basolateral membrane of rat distal colon. Evidence for Na-H exchange and Na-anion cotransport. J Clin Invest 88:1379–1385
Reinach PS, Schoen HF (1990) NPPB inhibits the basolateral membrane K+ conductance in the isolated bullfrog cornea. Biochim Biophys Acta 1026:13–20
Seifter JL, Aronson PS (1986) Properties and physiologic roles of the plasma membrane sodium-hydrogen exchanger. J Clin Invest 78:859–864
Singh AK, Afink GB, Venglarik CJ, Wang RP, Bridges RJ (1991) Colonic Cl channel blockade by three classes of compounds. Am J Physiol 261:C51-C63
Smith PL, Sullivan SK, McCabe RD (1986) Concentration-dependent effects of disulfonic stilbenes on colonic chloride transport. Am J Physiol 250:G44-G49
Warnock DG, Pollack AS (1988) Na+/H+ exchange in epithelial cells. In: Grinstein S (ed) Na+/H+ exchange. CRC Press, Boca Raton, pp 77–90
Welsh MJ, Smith PL, Fromm M, Frizzell RA (1982) Crypts are the site of intestinal water and electrolyte secretion. Science 218:1219–1221
Wiener H, van Os C (1989) Rabbit distal colon epithelium. II. Characterization of (Na+,K+,Cl−)-cotransport and [3]H]-bumetanide binding. J Membr Biol 110:163–174
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hatch, M., Freel, R.W. & Vaziri, N.D. Mechanisms of oxalate absorption and secretion across the rabbit distal colon. Pflugers Arch. 426, 101–109 (1994). https://doi.org/10.1007/BF00374677
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00374677