Abstract
Four treatments (control, chemical fertilizer, wheat straw, and wheat straw+fertilizer) were established on the dryland experimental farm of the Institute of Agricultural Sciences, Banaras Hindu University. Organic in C in the different treatments ranged from 0.69 to 0.93%, total N from 0.08 to 0.11%, and total P from 0.018 to 0.021. The application of straw significantly increased the soil water-holding capacity. The maximum effect on the microbial biomass was realized with the straw+fertilizer treatment, followed by straw and then by the fertilizer treatment. During the study microbial biomass C ranged from 144 to 491 μg g-1 dry soil, biomass N from 14.6 to 50.1 μg g-1, and biomass P from 7.2 to 17.6 μg g-1 soil. Microbial biomass C, N and P represented 3.2–4.6% of total C, 2.6–3.8% of total N, and 5.8–8.2% of total P in the soil, respectively, in all cases the highest proportion occurred in the straw+fertilizer treatment and the lowest in the control. Microbial biomass C, N, and P were positively correlated with each other. Microbial biomass C and N increased by 77% in straw+fertilizer-treated plots relative to the control. The increase in microbial biomass P in the straw+fertilizer treatment over the control was 81%. The increase in the microbial biomass is expected to enhance nutrient availability in the soil, as the microbial biomass acts both as a sink and a source of plant nutrients.
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References
Agarwal RR, Mehrotra CL (1952) Soil work in Uttar Pradesh, vol. II. Department of Agriculture, Uttar Pradesh, India
Allen SE, Grimshaw HM, Rowland AP (1976) Chemical analysis. In: Moore PD, Chapman SB (eds) Methods in plant ecology. Blackwell Scientific Publications, Oxford, pp 285–344
Anderson JPE Domsch KH (1980) Quantities of plant nutrients in the microbial biomass of selected soils. Soil Sci 130:211–216
Anderson T-H, Domsch KH (1989) Ratios of microbial biomass carbon to total organic carbon in arable soils. Soil Biol Biochem 21:471–479
Anderson JM, Ingram JSI (1989) Tropical soil biology and fertility: A handbook of methods. CAB International, Wallingford, UK
Azam F, Yousaf M, Hussain F, Malik KA (1989) Determination of biomass N in some agricultural soils of Punjab, Pakistan. Plant and Soil 113:223–228
Beare MH, Blair JM, Parmelle RW (1989) Resource quality and trophic responses to simulated throughfall: Effects on decomposition and nutrient flux in a no-tillage agroecosystem. Soil Biol Biochem 21:1027–1036
Beck T (1984) Mikrobiologische und biochemische Charakterisierung landwirtschaftlich genutzter Böden. II. Mitteilung. Beziehungen zum Humushaushalt. Z Pflanzenernaehr Bodenkd 147:467–475
Bonde TA, Schnurer J, Roswall T (1988) Microbial biomass as a fraction of potentially mineralizable nitrogen in soils from long-term field experiments. Soil Biol Biochem 20:447–452
Brookes PC, Powlson DS, Jenkinson DS (1982) Measurement of microbial biomass phosphorus in soil. Soil Biol Biochem 14:319–329
Brookes PC, Powlson DS, Jenkinson DS (1984) Phosphorus in soil microbial biomass. Soil Biol Biochem 16:169–175
Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem 17:837–842
Dalal RC, Mayer RJ (1987) Long-term trends in fertility of soils under continuous cultivation and cereal cropping in Southern Queensland. VII. Dynamics of nitrogen mineralization potentials and microbial biomass. Aust J Soil Res 25:461–472
Hendrix PF, Parmelee RW, Crossley DA Jr, Coleman DC, Odum EP, Groffman PM (1986) Detritus food webs and conventional and notillage agroecosystems. BioScience 36:374–380
Insam H, Parkinson D, Domsch KH (1989) Influence of macroclimate on soil microbial biomass. Soil Biol Biochem 21:211–221
Jackson ML (1958) Soil chemical analysis. Prentice-Hall, Englewood Cliffs, New Jersey
Jenkinson DS (1976) The effects of biocidal treatments on metabolism in soil. IV. The decomposition of fumigated organisms in soil. Soil Biol Biochem 8:203–208
Jenkinson DS (1988) The determination of microbial biomass carbon and nitrogen in soil. In: Wilson JR (ed) Advances in nitrogen cycling in agricultural ecosystems. CAB International, Wallingford, UK, pp 368–386
Jenkinson DS, Ladd JN (1981) Microbial biomass in soil measurement and turnover. In: Paul EA, Ladd JN (eds) Soil biochemistry, vol. 5. Marcel Dekker, New York, pp 415–471
Jenkinson DS, Rayner JH (1977) The turnover of soil organic matter in some of the Rothamsted classical experiments. Soil Sci 123:298–305
Ocio JA, Brookes PC (1990) An evaluation of methods for measuring the microbial biomass in soils following recent additions of wheat straw and the characterization of the biomass that develops. Soil Biol Biochem 22:685–694
Ocio JA, Martinez J, Brookes PC (1991) Contribution of straw-derived N to total microbial biomass N following incrorporation of cereal straw to soil. Soil Biol Biochem 23:171–176
Olsen SR, Cole CV, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate, United States Department of Agriculture Circ No 939. Government Printing Office, Washington, DC
Perrott KW, Sarathchandra SU (1989) Phosphorus in the microbial biomass of New Zealand soils under established pasture. NZ J Agric Res 32:409–413
Piper CS (1944) Soil and plant analysis. Interscience Publications Inc, New York
Powlson DS, Jenkinson DS (1981) A comparison of organic matter, biomass, adenosine triphosphate and mineralizable nitrogen contents of plowed and direct drilled soils. J Agric Sci 97:713–721
Powlson DS, Brookes PC, Cristensen BT (1987) Measurement of soil microbial biomass provides an early indication of changes in total soil organic matter due to straw incorporation. Soil Biol Biochem 19:159–164
Raghubanshi AS (1991) Dynamics of soil biomass C, N and P in a dry tropical forest in India. Biol Fertil Soils 12:55–59
Rauhe K (1987) Effects of organic manuring and cropping on soil humus and fertility. In: Welte E, Szabolcs I (eds) Agricultural waste management and environmental protection. 4th Int CIEC Symp Proc, Int Sci Centre Fert, Belgrade
Saffigna PG, Powlson DS, Brookes PC, Standley J, Thomas GA, Hunter HM (1984) Influence of tillage and sorghum residues on carbon, nitrogen and phosphorus in the soil microbial biomass of a Vertisol in central Queensland. In: Proc Nat Soils Conf, Brisbane. Aust Soc Soil Sci, Brisbane
Saffigna PG, Powlson DS, Brookes PC, Thomas GA (1989) Influence of sorghum residues and tillage on soil organic matter and soil microbial biomass in an Australian Vertisol. Soil Biol Biochem 21:759–765
Sarathchandra SU, Perrott KW, Upsdell MP (1984) Microbiological and biochemical characteristics of a range of New Zealand soils under established pasture. Soil Biol Biochem 16:177–183
Schnürer J, Clarholm M, Rosswall T (1985) Microbial biomass and activity in an agricultural soil with different organic matter contents. Soil Biol Biochem 17:611–618
Shen SM, Hart PBS, Powlson DS, Jenkinson DS (1989) The nitrogen cycle in the Broadbalk wheat experiment: 15N-labelled fertilizer residues in the soil and in the soil microbial biomass. Soil Biol Biochem 21:529–533
Singh H (1992) Effect of residue placement on the dynamics of microbial biomass C, N and P and crop growth under dryland farming conditions. PhD thesis, Banaras Hindu University, Varanasi, India
Smith JL, Paul EA (1990) The significance of soil microbial biomass estimations. In: Bollag JM, Stotzky G (eds) Soil biochemistry, vol 6, Marcel Dekker, New York, pp 357–396
Sparling GP, Whale KN, Ramsay AJ (1985) Quantifying the contribution from the soil microbial biomass to the extractable P levels of fresh and air-dried soils. Aust J Soil Res 23:613–621
Srivastava SC, Singh JS (1988) Carbon and phosphorus in the soil biomass of some tropical soils of India. Soil Biol Biochem 20:743–747
Srivastava SC, Singh JS (1989) Effect of cultivation on microbial carbon and nitrogen in dry tropical forest soil. Biol Fertil Soils 8:343–348
Srivastava SC, Jha AK, Singh JS (1989) Changes with time in soil biomass C, N and P of mine spoils in a dry tropical environment. Can J Soil Sci 69:849–855
Statistical Package for the Social Sciences (1986) SPSS/PC for the IBM PC/XT/AT. SPPS, Chicago, Illinois, U.S.A.
Swift MJ (1987) Tropical soil biology and fertility: Interregional research planning workshop. Biol Int Spec Iss 13; Int Union Biol Sci, Paris
Tate KR, Ross DJ, Feltham CW (1988) A direct extraction method to estimate soil microbial C: Effects of experimental variables and some different calibration procedures. Soil Biol Biochem 20:329–335
Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707
Walkley A (1947) A critical examination of a rapid method for determining organic carbon in soils — effects of variations in digestion conditions and of inorganic soil constituents. Soil Sci 63:251–264
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Singh, H., Singh, K.P. Effect of residue placement and chemical fertilizer on soil microbial biomass under tropical dryland cultivation. Biol Fert Soils 16, 275–281 (1993). https://doi.org/10.1007/BF00369304
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DOI: https://doi.org/10.1007/BF00369304