Abstract
End product inhibition can be reduced by the in situ removal of inhibitory fermentation products as they form. Extractive fermentation, in which an immiscible organic solvent is added to the fermentor in order to extract inhibitory products, was applied to the acetone-butanol fermentation. Six solvents or solvent mixtures were tested in batch extractive fermentations: kerosene, 30 wt% tetradecanol in kerosene, 50 wt% dodecanol in kerosene, oleyl alcohol, 50 wt% oleyl alcohol in a decane fraction and 50 wt% oleyl alcohol in benzyl benzoate. The best results were obtained with oleyl alcohol or a mixture of oleyl alcohol and benzyl benzoate. In normal batch fermentation of Clostridium acetobutylicum, glucose consumption is limited to about 80 kg/m3 due to the accumulation of butanol in the broth. In extractive fermentation using oleyl alcohol or a mixture of oleyl alcohol and benzyl benzoate, over 100 kg/m3 of glucose can be fermented. Removal of butanol from the broth as it formed also increased the rate of butanol production. Maximum volumetric butanol productivity was increased by as much as 60% in extractive fermentation compared to batch fermentation. Butanol productivities obtained in extractive fermentation compare favorably with other in situ product removal fermentations.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Hastings, J. J. H.: Acetone-butyl alcohol fermentation. In: Rose, A. H. (Ed.): Economic Microbiology, vol. 2, pp. 31–44. New York: Academic Press 1978
Walton, M. T.; Martin, J. L.: Production of butanol-acetone by fermentation. In: Peppier, H. J.; Perlman, D. (Eds.): Microbial Technology, vol. 1, pp. 187–209. New York: Academic Press 1979
Leung, J. C. Y.; Wang, D. I. C.: Production of acetone and butanol by Clostridium acetobutylicum in continuous culture using free cells and immobilized cells. In: Robinson, C. W. (Ed.): Proceedings of the 2nd World Congress of Chemical Engineering and World Chemical Exposition, pp. 348–352. Montreal, Canada, October 4–9, 1981
Murphy, T. K.; Blanch, H. W.; Wilke, C. R.: Water recycle in extractive fermentation. Process Biochem. 17 (1982) 6–9
Linden, J. C.; Moreira, A. R.; Lenz, T. G.: Acetone and butanol. In: Moo-Young, M. (Ed.): Comprehensive Biotechnology, vol. 3, pp. 915–931. New York: Pergamon Press 1985
Groot, W. J.; Van den Oever, C. E.; Kossen, N. W. F.: Pervaporation for simultaneous product recovery in the butanol/isopropanol batch fermentation. Biotechnol. Lett. 6 (1984) 709–714
Groot, W. J.; Schoutens, G. H.; Van Beelen, P. N.; Van den Oever, C. E.; Kossen, N. W. F.: Increase of substrate conversion by pervaporation in the continuous butanol fermentation. Biotechnol. Lett. 6 (1984) 789–792
Garcia, A.; Iannotti, E. L.; Fischer, J. R.: Reverse osmosis application for butanol-acetone fermentation. Biotechnol. Bioeng. Symp. No. 14 (1984) 543–552
Larsson, M.; Holst, O.; Mattiasson, B.: Butanol fermentation using a selective adsorbent for product recovery. In: Third European Congress on Biotechnology, vol. 2, pp. 313–316. München, Fed. Rep. of Germany, September 10–14, 1984
Heady, R. E.; Frankiewicz, J. R.: U.S. Patent 4,520,104 (1985)
Kuhn, I.: Alcoholic fermentation in an aqueous two-phase system. Biotechnol. Bioeng. 22 (1980) 2393–2398
Hahn-Hagerdal, B.; Mattiasson, B.; Albertsson, P.-A.: Extraction bioconversion in aqueous two phase systems. A model study on the conversion of cellulose to ethanol. Biotechnol. Lett. 3 (1981) 53–58
Mattiasson, B.; Suominen, M.; Andersson, E.; Haggstrom, L.; Albertsson, P.-A.; Hahn-Hagerdal, B.: Solvent production by Clostridium acetobutylicum in aqueous two-phase systems. In: Chibata, I.; Fukui, S.; Wingard Jr., L. B. (Eds.): Enzyme Engineering, vol. 6, pp. 153–155. New York: Plenum Press 1982
Mattiasson, B.; Hahn-Hagerdal, B.: Utilization of aqueous two-phase systems for generating soluble immobilized preparations of biocatalysts. In: Mattiasson, B. (Ed.): Immobilized cells and organelles, vol. 1, pp. 121–134. Boca Raton, Florida: CRC Press 1983
Hashimoto, Y.: Optimization of an extractive fermentation process for the production of butanol. M.Sc. Thesis, Univ. of Pennsylvannia, Philadelphia 1979
Ishii, S.; Taya, M.; Kobayashi, T.: Production of butanol by Clostridium acetobutylicum in extractive fermentation system. J. Chem. Engr. Japan. 18 (1985) 125–130
Taya, M.; Ishii, S.; Kobayashi, T.: Monitoring and control for extractive fermentation of Clostridium acetobutylicum. J. Ferment. Technol. 63 (1985) 181–187
Annual Technical Report of the Research Assoc. for Petroleum Alternatives Develop., Chiyoda-ku, Tokyo 101, Japan, September, 1985
Linden, J. C.; Paige, M. R.; Doremus, M. D.: Fed-Batch Fermentations of Clostridium acetobutylicum. Presented at the AIChE National Meeting, San Francisco, CA, November 27, 1984
Ennis, B. M.; Maddox, I. S.: Use of Clostridium acetobutylicum P262 for production of solvents from whey permeate. Biotechnol. Lett. 7 (1985) 601–606
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roffler, S.R., Blanch, H.W. & Wilke, C.R. In-situ recovery of butanol during fermentation. Bioprocess Engineering 2, 1–12 (1987). https://doi.org/10.1007/BF00369221
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00369221