Summary
Strains of the D-xylose fermenting yeast Pichia stipitis containing varying amounts of cellular DNA, were created through polyethylene glycol induced protoplast fusions. The majority of the fusant strains appeared to be very unstable and segregated easily to cells of lower DNA content, as typical of ploidy reduction. However, a few stable strains were obtained and their hybrid nature was confirmed by cell volume estimation, analysis of nuclear condition and the isolation of a variety of mutant phenotypic segregants by mitotic segregation as well as by meiotic segregation in some sporulating hybrids. The effect of gene dosage on ethanolic D-xylose fermentation was also investigated.
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Gupthar, A.S. Construction of a series of Pichia stipitis strains with increased DNA contents. Curr Genet 12, 605–610 (1987). https://doi.org/10.1007/BF00368063
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DOI: https://doi.org/10.1007/BF00368063