Summary
Analysis of a nucleotide sequence from pea chloroplast DNA which spans the site of deletion of one copy of the large inverted repeat (IR) element suggests that the IR unit has been entirely deleted, but that the single-copy coding sequences which flanked it (trnH and ndh5) have been left intact. However, these flanking sequences have not simply been ligated together: between them there is instead a novel ∼200 by DNA sequence which includes two regions apparently derived by the duplication of fragments of the psbA and rbcL genes.
Article PDF
Avoid common mistakes on your manuscript.
References
Bonnard G, Weil JH, Steinmetz A (1985) Curr Genet 9:417–422
Bowman CM, Dyer TA (1986) Curr Genet 10:931–941
Dewey RE, Levings III CS, Timothy DH (1986) Cell 44:439–449
Howe CJ (1985) Curr Genet 10:139–145
Ko K, Orfanides AG, Straus NA (1987) Theor Appl Genet 74:125–139
Lehmbeck J, Stummann BM, Henningsen KW (1987) Nucleic Acids Res 15:3630
Oishi KK, Shapiro DR, Tewari KK (1984) Mol Cell Biol 4:2556–2563
Palmer JD (1983) Nature 301:92–93
Palmer JD (1985) In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum Press, New York
Palmer JD, Osorio B, Aldrich J, Thompson WF (1987) Curr Genet 11:275–286
Quigley F, Weil JH (1985) Curr Genet 9:495–503
Shapiro DR, Tewari KK (1986) Plant Mol Biol 6:1–12
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Spielmann A, Stutz E (1983) Nucleic Acids Res 11:7157–7167
Zurawski G, Whitfield PR, Bottomley W (1986) Nucleic Acids Res 14:3975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wolfe, K.H. The site of deletion of the inverted repeat in pea chloroplast DNA contains duplicated gene fragments. Curr Genet 13, 97–99 (1988). https://doi.org/10.1007/BF00365763
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00365763